LOCUS       sequence-352669-        9773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Mouse Irf7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Halitzki E, Zahan T, Arh N, 
            Zimmermannova O, Ferreira AG, Li H, Karlsson S, Scheding S, Pereira 
            CF
  TITLE     Single-cell transcriptional profiling informs efficient 
            reprogramming of human somatic cells to cross-presenting dendritic 
            cells.
  JOURNAL   Sci Immunol. 2022 Mar 4;7(69):eabg5539. doi: 
            10.1126/sciimmunol.abg5539. Epub 2022 Mar 4.
  PUBMED    35245086
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2022-03-4"; volume: "7(69):eabg5539. doi"; pages: 
            " 10.1126/sciimmunol.abg5539. Epub 2022 Mar 4"
COMMENT     Sequence Label: pFUW-TetO-Irf7
FEATURES             Location/Qualifiers
     source          1..9773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(20..38,39..61,62..80,81..103,104..122,123..145,
                     146..164,165..187,188..206,207..229,230..248,249..271,
                     272..290)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        323..361
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     358..382
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1871..2459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1924..1944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2342..2353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2462..2478)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2463..2479)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2984..3164
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3190..3207)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3196..3420
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3466..3894
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3553..3572)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3763..3784
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3903..3923)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3908..4237
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4088..4223
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4150..4169
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4285..4332
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4351..4725
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4855..4976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4892..4911)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4946..4965
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5025..5041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5025..5041)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5038..5060)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5049..5065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5073..5079,5080..5097,5098..5103))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5118..5139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5256..5273)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5427..6015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5507..5526)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6186..6977,6978..7046))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6809..6828
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7047..7151)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7226..7245)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7417..7796
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7798..7996
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8014..8194
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8241..8366
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8859..9092
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9277..9321
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9619..9736
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
       61 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      121 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      181 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      241 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      301 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      361 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      421 aagacaccgg gaccgatcca gcctccgcgg ccccgaattc accggttcta gaatggctga
      481 agtgaggggg gtccagcgag tgctgtttgg agactggcta ttgggggagg tcagcagcgg
      541 ccagtacgag gggctgcagt ggctgaacga ggctcgcaca gtcttccgcg taccctggaa
      601 gcatttcggt cgtagggatc tggatgaaga agatgcacag atcttcaagg cctgggctgt
      661 ggcccgaggg aggtggccac ctagtggagt taacctgcca cccccagagg ctgaggctgc
      721 tgagcgaaga gagcgaagag gctggaagac caacttccgc tgtgcactcc acagcacagg
      781 gcgttttatc ttgcgccaag acaattcagg ggatccagtt gatccgcata aggtgtacga
      841 acttagccgg gagcttggat ctactgtggg cccagccacg gaaaataggg aagaagtgag
      901 cctcagcaat gctctgccca cacagggtgt gtccccagga tcatttctgg caagagaaaa
      961 tgctgggctc caaaccccaa gccctctgct ttctagtgat gccggggacc tcttgcttca
     1021 ggttctgcag tacagccaca tactggaatc cgagtctggg gcagaccccg tcccaccaca
     1081 ggctcctggc caggagcaag accgtgttta cgaggaaccc tatgcagcat ggcaggtgga
     1141 agctgtcccc agtcccaggc ctcaacagcc agctctcacc gagcgcagcc ttgggttcct
     1201 ggatgtgacc atcatgtaca agggccgcac agtgctacag gcagtggtgg ggcaccccag
     1261 atgcgtgttc ctgtacagcc ccatggcccc agcagtaaga acttcagagc cccagccggt
     1321 gatctttccc agtcctgctg agctcccaga tcagaagcag ctgcactaca cagagacgct
     1381 tctccagcat gtgtctcccg gccttcagct ggagcttcga ggaccgtcac tgtgggccct
     1441 gcgtatgggc aagtgcaagg tgtactggga ggtaggcagc cctatgggca ctaccggccc
     1501 ctccacccca ccccagctgc tggagcgcaa ccgccacacc cccatcttcg acttcagcac
     1561 tttcttccga gaactggagg agtttcgggc tcggaggcgg caagggtcac cacactacac
     1621 catctacctg ggttttgggc aagacttgtc agcagggagg cccaaggaga agaccctgat
     1681 cctggtgaag ctggagccat gggtatgcaa ggcatacctg gagggcgtgc agcgtgaggg
     1741 tgtgtcctcc ctggacagca gcagtctcgg cttgtgcttg tctagcacca acagtctcta
     1801 cgaagacatc gaacacttcc tcatggacct gggtcagtgg ccttgattcg aagatatcaa
     1861 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     1921 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     1981 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     2041 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     2101 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     2161 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     2221 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     2281 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     2341 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     2401 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     2461 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     2521 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     2581 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     2641 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     2701 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     2761 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     2821 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     2881 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     2941 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     3001 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3061 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3121 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     3181 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3241 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3301 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     3361 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     3421 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     3481 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3541 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3601 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3661 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3721 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3781 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3841 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     3901 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3961 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4021 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4081 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4141 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4201 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4261 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     4321 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     4381 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     4441 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     4501 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     4561 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     4621 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     4681 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     4741 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4801 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     4861 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4921 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4981 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     5041 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5101 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5161 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5221 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5281 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5341 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6061 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6121 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6181 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6241 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6301 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6421 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6481 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6601 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6661 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6841 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6901 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7021 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7081 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7141 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     7201 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7261 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     7321 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     7381 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     7441 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     7501 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     7561 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     7621 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     7681 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     7741 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     7801 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     7861 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     7921 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     7981 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     8041 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8101 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8161 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     8221 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     8281 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     8341 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     8401 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     8461 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     8521 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     8581 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8641 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8701 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8761 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8821 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8881 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     8941 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9001 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9061 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9121 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9181 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9241 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9301 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9361 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     9421 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     9481 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     9541 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     9601 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     9661 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     9721 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggt
//
