LOCUS       sequence-352759-        8781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.C.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        57..161
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(162..230,231..1022)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(380..399)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1193..1781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1682..1701
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1935..1952
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2069..2090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2105..2110,2111..2128,2129..2135)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2143..2159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2148..2170
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2167..2183
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2167..2183
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2202..2222
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2204..2222
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2250..2476
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2477..2657
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2704..2829
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3322..3555
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3740..3784
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4082..4330
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4082..4102
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4253..4272
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4349..4424
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4472..4589
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4729..5907
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4960..5898
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5855..5875
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5956..5965
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6004..6039
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6058..6099
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6109..6816
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6254..6272)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6522..6541
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6886..7474
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6939..6959)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7357..7368)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7549..7782
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7854..7975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7891..7910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7945..7964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8015..8150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8170..8189)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8171..8189)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8199..8216)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8199..8215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8208..8230)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8357..8781,1..31)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8444..8463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8654..8675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
       61 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      121 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      181 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      241 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
      301 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
      361 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
      421 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
      481 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
      541 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
      601 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
      661 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
      721 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
      781 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
      841 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
      901 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      961 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     1021 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1081 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1141 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1201 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1261 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1321 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1381 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1441 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1501 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1561 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1681 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1741 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1801 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1861 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1921 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1981 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     2041 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     2101 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     2161 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     2221 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     2281 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     2341 gcgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     2401 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     2461 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     2521 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     2581 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     2641 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     2701 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     2761 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     2821 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     2881 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     2941 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     3001 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     3061 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     3121 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     3181 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     3241 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     3301 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     3361 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     3421 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     3481 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     3541 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     3601 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     3661 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     3721 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     3781 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     3841 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     3901 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     3961 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     4021 agtgaacgga tctcgacggt atcgatcacg agactagtat cgagcggccg cccccttcac
     4081 cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     4141 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     4201 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     4261 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     4321 acgaaacacc gggtcttcga gaagacctgt tttagagcta gaaatagcaa gttaaaataa
     4381 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt taattctcga
     4441 cctcgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     4501 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     4561 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     4621 tttggccgcg gctcgaggcg ataagctttg caaagatgga taaagtttta aacagagagg
     4681 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
     4741 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     4801 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     4861 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     4921 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
     4981 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
     5041 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
     5101 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     5161 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     5221 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     5281 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     5341 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     5401 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     5461 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     5521 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     5581 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     5641 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     5701 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     5761 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     5821 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     5881 ttttttcttc catttcaggt gtcgtgagga attctgcagt cgatcgacgg taccgcgggc
     5941 ccgggatcca cggtcgccac catgggcgac acctatcggt acattggagg aggcggggga
     6001 ggggaggacc aggtggaccc caggctgatc gacggcaagg gaggaggcgg gggagggggc
     6061 aaaccaattc caaacccact gctgggactg gattccacag gcatgctgat ggtgagcaag
     6121 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     6181 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     6241 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     6301 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     6361 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     6421 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     6481 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     6541 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     6601 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     6661 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     6721 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     6781 cgccactcca ccggcggcat ggacgagctg tacaagaaac ggccagccga ggatatggag
     6841 gaagaacaag cctttaaacg atctcgataa agcggccgcg tcgacaatca acctctggat
     6901 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6961 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7021 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7081 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7141 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7201 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7261 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     7321 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7381 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc atcttcgcct tcgccctcag
     7441 acgagtcgga tctccctttg ggccgcctcc ccgcctgggt acctttaaga ccaatgactt
     7501 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7561 ttcactccca acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7621 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7681 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7741 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7801 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     7861 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7921 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7981 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8041 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8101 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     8161 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     8221 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8281 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8341 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8401 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8461 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8521 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     8581 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8641 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     8701 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     8761 atttaacaaa aatttaacgc g
//
