LOCUS       sequence-352761-        8757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.HA.NWS_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        63..167
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(168..236,237..1028)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(386..405)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1199..1787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1688..1707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1941..1958
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2075..2096
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2111..2116,2117..2134,2135..2141)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2149..2165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2154..2176
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2173..2189
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2173..2189
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2208..2228
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2210..2228
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2256..2482
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2483..2663
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2710..2835
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3328..3561
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3746..3790
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4088..4336
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4088..4108
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4259..4278
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4355..4430
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4478..4595
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4735..5913
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4966..5904
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5861..5881
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5962..5971
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6010..6036
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6058..6081
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6091..6798
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6236..6254)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6504..6523
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6868..7456
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6921..6941)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7339..7350)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7531..7764
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7836..7957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7873..7892)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7927..7946
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7997..8132
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8152..8171)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8153..8171)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8181..8198)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8181..8197)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8190..8212)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8339..8757,1..37)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8426..8445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8636..8657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
       61 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      121 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      181 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      241 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      301 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      361 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      421 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      481 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      541 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      601 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      661 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
      721 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
      781 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
      841 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
      901 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
      961 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1021 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1081 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1141 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1201 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1261 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1321 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1381 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1441 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1501 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1561 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1621 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1681 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1741 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1801 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     1861 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     1921 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     1981 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2041 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     2101 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     2161 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     2221 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     2281 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     2341 tgcatggcga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     2401 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     2461 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     2521 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     2581 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     2641 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     2701 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     2761 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     2821 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     2881 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     2941 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     3001 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     3061 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     3121 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     3181 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     3241 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     3301 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     3361 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     3421 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     3481 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     3541 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     3601 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     3661 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     3721 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     3781 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     3841 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     3901 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     3961 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     4021 tcgattagtg aacggatctc gacggtatcg atcacgagac tagtatcgag cggccgcccc
     4081 cttcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     4141 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     4201 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     4261 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     4321 gaaaggacga aacaccgggt cttcgagaag acctgtttta gagctagaaa tagcaagtta
     4381 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttaat
     4441 tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     4501 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     4561 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     4621 atccactttg gccgcggctc gaggcgataa gctttgcaaa gatggataaa gttttaaaca
     4681 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc
     4741 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4801 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     4861 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     4921 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     4981 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     5041 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     5101 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     5161 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     5221 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     5281 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     5341 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     5401 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     5461 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     5521 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     5581 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     5641 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     5701 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     5761 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     5821 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     5881 caaagttttt ttcttccatt tcaggtgtcg tgaggaattc tgcagtcgat cgacggtacc
     5941 gcgggcccgg gatccacggt cgccaccatg ggcgacacct atcggtacat tggaggaggc
     6001 gggggagggt acccatacga tgtgccagat tacgctggag gaggcggggg agggaactgg
     6061 tcccaccccc aattcgagaa aggcatgctg atggtgagca agggcgagga ggataacatg
     6121 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     6181 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     6241 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     6301 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     6361 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     6421 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     6481 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     6541 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     6601 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     6661 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     6721 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     6781 atggacgagc tgtacaagaa acggccagcc gaggatatgg aggaagaaca agcctttaaa
     6841 cgatctcgat aaagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga
     6901 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6961 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7021 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7081 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7141 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7201 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7261 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7321 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7381 ccggctctgc ggcctcttcc gcatcttcgc cttcgccctc agacgagtcg gatctccctt
     7441 tgggccgcct ccccgcctgg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7501 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat
     7561 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7741 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     7801 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     7861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     7981 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8041 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8101 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     8161 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8221 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8281 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     8341 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8401 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8461 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8521 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8581 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8641 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     8701 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaattt
//
