LOCUS       sequence-352764-        8763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.HA.VSVg_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(211..279,280..1071)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(429..448)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2118..2139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2154..2159,2160..2177,2178..2184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2192..2208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2197..2219
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2216..2232
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2216..2232
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2251..2271
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2253..2271
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2299..2525
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2526..2706
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2753..2878
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3371..3604
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3789..3833
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4131..4379
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4131..4151
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4302..4321
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4398..4473
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4521..4638
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4778..5956
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5009..5947
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5904..5924
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6005..6014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6053..6079
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6098..6130
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6140..6847
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6285..6303)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6553..6572
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6917..7505
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6970..6990)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7388..7399)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7580..7813
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7885..8006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7922..7941)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7976..7995
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8046..8181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8201..8220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8202..8220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8230..8247)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8230..8246)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8239..8261)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8388..8763,1..80)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8475..8494)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8685..8706
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
       61 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      421 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
      481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      721 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
      781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
      841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
      901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
      961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     2161 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     2221 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     2281 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     2341 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgg cgattggtgg
     2401 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     2461 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     2521 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     2581 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     2641 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     2701 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     2761 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     2821 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     2881 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     2941 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     3001 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     3061 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     3121 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     3181 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     3241 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     3301 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     3361 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     3421 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     3481 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     3541 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     3601 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     3661 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     3721 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     3781 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     3841 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     3901 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     3961 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     4021 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     4081 ctcgacggta tcgatcacga gactagtatc gagcggccgc ccccttcacc gagggcctat
     4141 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
     4201 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
     4261 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
     4321 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
     4381 ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     4441 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt aattctcgac ctcgagacaa
     4501 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     4561 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     4621 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg
     4681 ctcgaggcga taagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
     4741 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
     4801 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     4861 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     4921 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     4981 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     5041 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     5101 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     5161 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     5221 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     5281 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     5341 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     5401 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     5461 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     5521 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     5581 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     5641 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     5701 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     5761 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     5821 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     5881 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     5941 atttcaggtg tcgtgaggaa ttctgcagtc gatcgacggt accgcgggcc cgggatccac
     6001 ggtcgccacc atgggcgaca cctatcggta cattggagga ggcgggggag ggtacccata
     6061 cgatgtgcca gattacgctg gaggaggcgg gggagggtat acagatatcg aaatgaatag
     6121 actcggaaag ggcatgctga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     6181 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     6241 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     6301 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     6361 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     6421 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     6481 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     6541 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     6601 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     6661 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     6721 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     6781 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     6841 gtacaagaaa cggccagccg aggatatgga ggaagaacaa gcctttaaac gatctcgata
     6901 aagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6961 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7021 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7081 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7141 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7201 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7261 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7321 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7381 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7441 gcctcttccg catcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7501 cccgcctggg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7561 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata agatctgctt
     7621 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7681 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7741 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7801 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7861 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7921 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7981 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     8041 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8101 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8161 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     8221 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8281 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8341 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8401 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8461 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8521 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8581 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8641 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8701 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8761 gat
//
