LOCUS       sequence-352773-        8781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.HSV.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(95..163,164..955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(313..332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1126..1714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1615..1634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1868..1885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2002..2023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2038..2043,2044..2061,2062..2068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2076..2092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2081..2103
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2100..2116
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2100..2116
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2135..2155
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2137..2155
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2183..2409
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2410..2590
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2637..2762
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3255..3488
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3673..3717
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4015..4263
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4015..4035
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4186..4205
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4282..4357
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4405..4522
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4662..5840
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4893..5831
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5788..5808
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5889..5898
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5937..5969
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             5988..6032
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6042..6749
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6187..6205)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6455..6474
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6819..7407
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6872..6892)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7290..7301)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7482..7715
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7787..7908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7824..7843)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7878..7897
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7948..8083
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8103..8122)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8104..8122)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8132..8149)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8132..8148)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8141..8163)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8290..8745
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8377..8396)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8587..8608
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(8771..8781,1..94)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
       61 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      121 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      181 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      241 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      301 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      361 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      421 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
      481 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
      541 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
      601 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
      661 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
      721 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
      781 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
      841 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
      901 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
      961 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1021 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1081 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1141 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1201 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1261 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1321 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1381 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1441 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1501 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1561 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1621 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1681 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1741 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1801 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     1861 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     1921 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     1981 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     2041 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     2101 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     2161 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     2221 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atggcgattg
     2281 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     2341 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     2401 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2461 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     2521 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     2581 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     2641 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     2701 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     2761 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     2821 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     2881 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     2941 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     3001 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     3061 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     3121 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     3181 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     3241 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     3301 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     3361 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     3421 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     3481 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     3541 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     3601 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     3661 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     3721 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     3781 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     3841 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     3901 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     3961 ggatctcgac ggtatcgatc acgagactag tatcgagcgg ccgccccctt caccgagggc
     4021 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     4081 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     4141 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     4201 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     4261 accgggtctt cgagaagacc tgttttagag ctagaaatag caagttaaaa taaggctagt
     4321 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttaattct cgacctcgag
     4381 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     4441 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     4501 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
     4561 gcggctcgag gcgataagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt
     4621 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     4681 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     4741 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     4801 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     4861 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     4921 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     4981 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     5041 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     5101 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     5161 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     5221 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     5281 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     5341 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     5401 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     5461 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     5521 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     5581 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     5641 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     5701 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     5761 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     5821 ttccatttca ggtgtcgtga ggaattctgc agtcgatcga cggtaccgcg ggcccgggat
     5881 ccacggtcgc caccatgggc gacacctatc ggtacattgg aggaggcggg ggagggcagc
     5941 ccgagctggc ccccgaggac cccgaggacg gaggaggcgg gggagggaag gagaccgccg
     6001 ccgccaagtt cgagaggcag cacatggaca gcggcatgct gatggtgagc aagggcgagg
     6061 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     6121 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     6181 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     6241 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     6301 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     6361 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     6421 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     6481 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     6541 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     6601 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     6661 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     6721 ccaccggcgg catggacgag ctgtacaaga aacggccagc cgaggatatg gaggaagaac
     6781 aagcctttaa acgatctcga taaagcggcc gcgtcgacaa tcaacctctg gattacaaaa
     6841 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6901 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6961 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7021 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7081 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7141 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7201 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     7261 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7321 gcggcctgct gccggctctg cggcctcttc cgcatcttcg ccttcgccct cagacgagtc
     7381 ggatctccct ttgggccgcc tccccgcctg ggtaccttta agaccaatga cttacaaggc
     7441 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7501 ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     7561 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7621 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7681 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     7741 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7801 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7861 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     7921 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7981 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8041 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     8101 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     8161 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     8221 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     8281 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     8341 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     8401 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     8461 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     8521 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8581 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8641 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8701 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     8761 gggaaatgtg cgcggaaccc c
//
