LOCUS       sequence-352827-        8790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.Ollas.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        116..220
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(221..289,290..1081)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(439..458)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1252..1840
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1741..1760
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1994..2011
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2128..2149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2164..2169,2170..2187,2188..2194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2202..2218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2207..2229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2226..2242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2226..2242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2261..2281
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2263..2281
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2309..2535
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2536..2716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2763..2888
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3381..3614
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3799..3843
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4141..4389
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4141..4161
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4312..4331
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4408..4483
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4531..4648
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4788..5966
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5019..5957
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5914..5934
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6015..6024
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6063..6104
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6126..6167
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6177..6884
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6322..6340)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6590..6609
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6954..7542
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7007..7027)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7425..7436)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7617..7850
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7922..8043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7959..7978)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8013..8032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8083..8218
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8238..8257)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8239..8257)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8267..8284)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8267..8283)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8276..8298)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8425..8790,1..90)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8512..8531)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8722..8743
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
       61 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
      121 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      181 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      241 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      301 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      361 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      421 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      481 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      541 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      601 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
      661 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
      721 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
      781 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
      841 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
      901 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
      961 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     1021 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1081 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1141 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1201 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1261 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1321 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1381 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1441 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1501 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1561 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1621 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1681 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1741 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1801 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1861 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1921 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1981 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     2041 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     2101 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     2161 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     2221 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     2281 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     2341 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgg
     2401 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     2461 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     2521 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2581 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2641 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2701 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     2761 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     2821 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     2881 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     2941 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     3001 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     3061 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     3121 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     3181 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     3241 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     3301 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     3361 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     3421 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     3481 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     3541 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     3601 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     3661 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     3721 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     3781 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     3841 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     3901 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     3961 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     4021 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     4081 gtgaacggat ctcgacggta tcgatcacga gactagtatc gagcggccgc ccccttcacc
     4141 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     4201 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     4261 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     4321 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     4381 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
     4441 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt aattctcgac
     4501 ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     4561 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     4621 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     4681 ttggccgcgg ctcgaggcga taagctttgc aaagatggat aaagttttaa acagagagga
     4741 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc
     4801 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     4861 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     4921 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     4981 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     5041 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     5101 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     5161 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     5221 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     5281 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     5341 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     5401 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     5461 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     5521 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     5581 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     5641 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     5701 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     5761 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     5821 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     5881 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     5941 tttttcttcc atttcaggtg tcgtgaggaa ttctgcagtc gatcgacggt accgcgggcc
     6001 cgggatccac ggtcgccacc atgggcgaca cctatcggta cattggagga ggcgggggag
     6061 ggagcggctt cgccaacgag ctgggcccca ggctgatggg caagtgcgga ggaggcgggg
     6121 gagggggcaa accaattcca aacccactgc tgggactgga ttccacaggc atgctgatgg
     6181 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     6241 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     6301 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     6361 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     6421 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     6481 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     6541 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     6601 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     6661 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     6721 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     6781 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     6841 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagaaacgg ccagccgagg
     6901 atatggagga agaacaagcc tttaaacgat ctcgataaag cggccgcgtc gacaatcaac
     6961 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7021 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7081 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7141 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7201 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7261 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7321 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     7381 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7441 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcat cttcgccttc
     7501 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgggtac ctttaagacc
     7561 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7621 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     7681 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7741 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7801 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7861 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7921 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7981 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8041 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     8101 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8161 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8221 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     8281 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8341 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8401 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8461 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8521 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8581 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8641 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8701 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     8761 tctcggtcta ttcttttgat ttataaggga
//
