LOCUS       sequence-352838-        8793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.HA.Ollas_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..153
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(154..222,223..1014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(372..391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1185..1773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1674..1693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1927..1944
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2061..2082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2097..2102,2103..2120,2121..2127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2135..2151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2140..2162
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2159..2175
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2159..2175
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2194..2214
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2196..2214
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2242..2468
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2469..2649
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2696..2821
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3314..3547
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3732..3776
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4074..4322
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4074..4094
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4245..4264
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4341..4416
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4464..4581
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4721..5899
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4952..5890
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5847..5867
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5948..5957
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5960..5995
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6014..6040
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6059..6100
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6113..6820
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6258..6276)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6526..6545
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6890..7478
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6943..6963)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7361..7372)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7553..7786
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7858..7979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7895..7914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7949..7968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8019..8154
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8174..8193)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8175..8193)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8203..8220)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8203..8219)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8212..8234)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8361..8789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8448..8467)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8658..8679
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
       61 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      121 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      181 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      241 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      301 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      361 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      421 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      481 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
      541 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
      601 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
      661 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
      721 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
      781 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
      841 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
      901 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
      961 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1021 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1081 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1141 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1201 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1261 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1321 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1381 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1441 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1501 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1561 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1621 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1681 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1741 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1801 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1861 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     1921 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     1981 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2041 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     2101 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     2161 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     2221 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     2281 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tggcgattgg
     2341 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     2401 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     2461 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     2521 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     2581 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     2641 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     2701 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     2761 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     2821 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     2881 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     2941 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     3001 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     3061 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     3121 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     3181 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     3241 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     3301 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     3361 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     3421 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     3481 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     3541 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     3601 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     3661 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     3721 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     3781 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     3841 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     3901 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     3961 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     4021 gatctcgacg gtatcgatca cgagactagt atcgagcggc cgcccccttc accgagggcc
     4081 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     4141 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     4201 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     4261 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     4321 ccgggtcttc gagaagacct gttttagagc tagaaatagc aagttaaaat aaggctagtc
     4381 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttaattctc gacctcgaga
     4441 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     4501 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     4561 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
     4621 cggctcgagg cgataagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
     4681 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
     4741 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     4801 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     4861 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     4921 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     4981 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     5041 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     5101 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     5161 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     5221 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     5281 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     5341 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     5401 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     5461 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     5521 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     5581 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     5641 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     5701 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     5761 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     5821 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     5881 tccatttcag gtgtcgtgag gaattctgca gtcgatcgac ggtaccgcgg gcccgggatc
     5941 cacggtcgcc accatgggcg aggaccaggt ggaccccagg ctgatcgacg gcaagggagg
     6001 aggcggggga gggtacccat acgatgtgcc agattacgct ggaggaggcg ggggagggag
     6061 cggcttcgcc aacgagctgg gccccaggct gatgggcaag tgcggcatgc tgatggtgag
     6121 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     6181 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     6241 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     6301 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     6361 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     6421 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     6481 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     6541 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     6601 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     6661 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     6721 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     6781 gggccgccac tccaccggcg gcatggacga gctgtacaag aaacggccag ccgaggatat
     6841 ggaggaagaa caagccttta aacgatctcg ataaagcggc cgcgtcgaca atcaacctct
     6901 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6961 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7021 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7081 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7141 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7201 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7261 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     7321 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7381 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcatcttc gccttcgccc
     7441 tcagacgagt cggatctccc tttgggccgc ctccccgcct gggtaccttt aagaccaatg
     7501 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7561 ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc tctctggtta
     7621 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7681 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7741 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7801 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7981 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8041 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8101 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     8161 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     8221 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     8281 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     8341 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     8401 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     8461 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     8521 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     8581 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     8641 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     8701 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     8761 gctgatttaa caaaaattta acgcgaattt taa
//
