LOCUS       sequence-352934-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cargo EGFP with  AttP Bxb1 site (Parent minicircle).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, 
            Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, Zhang L, 
            Vakulskas CA, Walker JA 2nd, Kadina AP, Zepeda AE, Holden K, Ma H, 
            Xie J, Gao G, Foquet L, Bial G, Donnelly SK, Miyata Y, Radiloff DR, 
            Henderson JM, Ujita A, Abudayyeh OO, Gootenberg JS
  TITLE     Drag-and-drop genome insertion of large sequences without 
            double-strand DNA cleavage using CRISPR-directed integrases.
  JOURNAL   Nat Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4.
  PUBMED    36424489
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4."
COMMENT     Sequence Label: pDY0181 Cargo EGFP with AttP Bxb1 site (Parent...
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..115
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    complement(133..166)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    214..247
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     CDS             268..285
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgccgttcag ggtgccggtg atgcggcact cgatcttcat ggcgggcatg gtggcgaccg
       61 gtagctgggt ttgtaccgta caccactgag accgcggtgg ttgaccagac aaaccaccgc
      121 tagcggcttc ggataacttc gtatagcata cattatacga acggtaagcg ctaccgccgg
      181 catacccaag tgaagttgct cgcagcttat agtcgcgccc ggggagccca agggcacgcc
      241 ctggcaccgc ggccgctgag tctcgaccat catcatcatc atcattgagt ttatctggga
      301 taacagggta atgtcatcta gggataacag ggtatgtcat ctgggataac agggtaatgt
      361 atctagggat aacagggtaa tgtcatctgg gataacaggg taatgtcatc tagggataac
      421 agggtatgtc atctgggata acagggtaat gtatctaggg ataacagggt aatgtcaaac
      481 ggtctccagc ttggctgttt tggcggatga gagaagaatc gccagcgtcg cacagcatgt
      541 ttgcttgtcg ccgtcgcgtc tgtcacatct tttccgccag cagttaggga ttagcgtctt
      601 aagctggcgc gaggaccaac gtatcagcca ggcgaagctg cttttgagca ccacccggat
      661 gcctatcgcc accgtcggtc gcaatgttgg ttttgacgat caactctatt tctcgcgggt
      721 atttaaaaaa tgcaccgggg ccagcccgag cgagttccg
//
