LOCUS       sequence-352987-       12394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12394)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0331
FEATURES             Location/Qualifiers
     source          1..12394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5332..5920
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5385..5405)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5803..5814)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5992..6225
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6303..6424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6340..6359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6394..6413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6464..6599
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6619..6638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6620..6638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6648..6665)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6648..6664)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6657..6679)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6806..7261
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6893..6912)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7103..7124
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7287..7391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7392..7460,7461..8252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7610..7629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8423..9011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8912..8931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9165..9182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9299..9320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9335..9340,9341..9358,9359..9365)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9373..9389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9378..9400
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9397..9413
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9397..9413
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9432..9452
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9434..9452
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9480..9706
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9707..9887
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9934..10059
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10552..10785
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10970..11014
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11245..11455
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11267..11463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11314..11449
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11495..12097
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11495..11514)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11991..12011
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12140..12257
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 ggagaacgag gtggagagca gcagcgacgc ggcccctggg cctggccggc ccgaggagcc
     1261 ctctgagagc ggcctgggtg tgggcacctc agaagccgtg tccgccgaca gcagcgacgc
     1321 cgcggccgcc ccggggcagg cagaggccga tgactctggc gtggggcaaa gctcggaccg
     1381 cggcagccgt tctcaggagg aggtatctga gagcagctcg agcgcagacc ccctgcctaa
     1441 tagctacctc cctgattcat cgtctgtgtc tcatgggcca gtggcagggg tgacaggcgg
     1501 tcccccagca cttgtgcact ctagtgcact cccagacccc aacatgctgg tgtccgactg
     1561 cacagcttcc tcctcggacc tgggctcggc catcgacaag atcatcgagt ccaccatcgg
     1621 gcccgacctc atccagaact gcatcactgt gaccagtgct gaggatggcg gggccgagac
     1681 cacacggtac ctgatcctac agggcccaga tgatggagcc cccatgacat caccaatgtc
     1741 cagttccacc ttggcccaca gcctagcagc cattgaggcc ctggcagatg gccccacatc
     1801 cacatccaca tgcctggagg cacagggtgg gcccagctcc ccggtgcagc tgcccccagc
     1861 ctccggtgcc gaagagccgg acctgcagag cctggaggcc atgatggagg tggtggtggt
     1921 gcagcagttc aaatgcaaga tgtgccagta ccggagcagc accaaggcca cactgctgcg
     1981 ccacatgcgg gaacgccact tccgtccagt agcagcagcc gcagcagcag ctggtaaaaa
     2041 aggacgtcta cggaagtgga gcacctccac caagagccaa gaggaagagg gaccagagga
     2101 ggaggacgat gatgacattg tagacgctgg agccattgat gacctggagg aggatagcga
     2161 ctataatcca gctgaggatg agccccgagg ccggcagctt cggctccagc gccccacccc
     2221 cagtacccca aggccccgaa ggagacctgg ccggccccgg aagctgcccc gcctggagat
     2281 ctcagacctc ccagatggtg tggaaggaga gcctctagtg agttcccaga gtggacagag
     2341 ccctccagag ccacaggatc ccgaggctcc cagctcctca ggcccaggac acctggtggc
     2401 catgggcaag gtgagcagga cccctgtgga agctggtgtg agccagtcag atgcagagaa
     2461 cgcagccccc tcctgcccgg atgagcatga cactctgccc cggcgccgag gtcgaccttc
     2521 caggcgcttc ctaggcaaga aataccgcaa gtactattac aagtcgccca aaccactttt
     2581 gaggcccttc ctgtgccgca tctgtggttc tcgctttctg tcccacgagg acctgcgctt
     2641 ccacgtcaac tcccatgagg ctggcgatcc ccagctcttc aagtgcctgc agtgcagcta
     2701 tcgttcccgc cgctggtcct cgctcaagga gcacatgttc aaccacgtgg gcagcaagcc
     2761 ctacaagtgt gacgagtgca gctacaccag tgtctaccgg aaggacgtca ttcggcacgc
     2821 cgctgtgcac agccgggacc ggaagaagag gccagatccg actccaaagc tgagctcttt
     2881 cccctgccct gtgtgtggcc gtgtgtaccc catgcagaaa agactcacgc agcacatgaa
     2941 gacgcacagc actgagaagc cccacatgtg tgacaagtgt ggaaagtcct ttaagaagcg
     3001 ctacaccttc aaaatgcacc tgctcacgca catccaggct gttgccaacc gcaggttcaa
     3061 gtgtgagttc tgtgagtttg tttgtgaaga caagaaggca ctgctgaacc accagttgtc
     3121 ccacgtcagt gacaagccct tcaaatgcag cttttgtccc taccgcacct tccgagagga
     3181 cttcttgctg tcccatgtgg ctgtcaagca cacaggggcc aagcccttcg cctgtgagta
     3241 ctgccacttc agcacacggc acaagaagaa cctgcgcctg cacgtacggt gccgacacgc
     3301 aagcagcttc gaggaatggg ggaggcgcca ccctgaggag cccccctccc gccgtcgccc
     3361 cttcttctct ctgcagcaga ttgaggagct gaagcagcag cacagtgcgg cccctggacc
     3421 acctcccagt tccccaggac ctcctgagat acccccagag gcgacaactt tccagtcatc
     3481 tgaggctccc tcattgctct gttctgacac cctgggcggc gccaccatca tctaccagca
     3541 aggagctgag gagtcgacag cgatggccac gcagacagcc ttggatcttc tgctgaacat
     3601 gagtgctcag cgggaactgg ggggcacagc cctgcaggtg gctgtggtga agtcggaaga
     3661 tgtggaagca gggttagcat cccctggtgg gcagccctcc cctgaaggtg ccactccaca
     3721 ggtggtcacc ctccacgtgg cagagccagg gggcggtgca gcagccgaga gccagctagg
     3781 ccctcctgac ctaccgcaga tcaccctggc acctggtcca tttggtggga ctggctacag
     3841 tgtcatcaca gcacccccta tggaggaggg aacatcagct cctggcacac cttacagcga
     3901 ggagcccgca ggagaggcag cccaggctgt ggttgtgagt gacaccctaa aagaagctgg
     3961 cacccactac atcatggcta ctgatggtac ccagttgcac cacattgagc tcaccgcaga
     4021 tggctccatc tccttcccaa gtccagatgc tctggcctct ggtgccaaat ggcccctgct
     4081 gcagtgtggg ggactgccca gagacggccc tgagccccca tctccagcca agacccactg
     4141 cgtaggggac tcccagagct ctgcctcctc acctcctgca accagcaaag ccctgggcct
     4201 ggcagtgccc ccgtcaccgc catctgcagc cactgctgca tcaaagaagt tttcctgcaa
     4261 gatctgtgcc gaggccttcc ctggccgagc tgagatggag agtcacaagc gggcccacgc
     4321 tgggcctggt gccttcaagt gccccgactg ccccttcagt gcccgccagt ggcccgaggt
     4381 ccgggcgcac atggcacagc actcaagcct acggccccac cagtgtagcc agtgcagctt
     4441 tgcctccaag aacaagaagg acctgcgtcg gcacatgctg actcacacaa aggagaagcc
     4501 ttttgcatgc cacctctgcg ggcagcgttt caaccgtaac gggcacctca agttccacat
     4561 ccagcggctg cacagtcctg atgggaggaa gtcaggaacc cctacagccc gggcccctac
     4621 ccagacccca acccagacca tcatcctgaa cagtgatgac gaaacactgg ccaccctgca
     4681 cactgcactc cagtccagtc acggggtcct gggcccagag cggctacagc aggcactgag
     4741 ccaggaacac atcatcgttg cccaggaaca gacagtgacc aatcaggagg aagccgccta
     4801 catccaagag atcaccacgg cagatggcca gaccgtacag cacctggtga cctccgacaa
     4861 ccaggtgcag tatatcatct cccaggatgg tgtccagcac ctgctccccc aggaatatgt
     4921 tgtggtccct gaaggccatc acatccaggt acaggagggc cagatcacac acatccagta
     4981 tgaacaagga gccccgttcc ttcaggagtc ccagatccag tatgtgcctg tgtccccagg
     5041 ccagcagctt gtcacacagg ctcaacttga ggctgcagca cactcagctg tcacagcagt
     5101 ggctgatgct gccatggccc aagcccaggg cctgtttggt acagacgaga cagtgcccga
     5161 acacattcaa cagctgcagc accagggcat cgagtacgac gtcatcaccc tggccgatga
     5221 ctagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     5281 tggaaaggac gaaccgtccg gtctttatta cccgtcacgc gttaagtcga caatcaacct
     5341 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5401 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5461 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5521 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5581 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5641 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5701 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5761 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5821 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5881 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     5941 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6001 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6061 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6121 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6181 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     6241 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6301 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6421 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     6481 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6541 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6601 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     6661 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     6721 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     6781 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6841 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6901 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6961 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7021 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7081 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7141 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7201 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7261 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7621 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7921 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9061 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9121 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9181 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9241 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9301 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     9361 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     9421 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     9481 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     9541 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     9601 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     9661 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     9721 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9781 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9841 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     9901 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     9961 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
    10021 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
    10081 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
    10141 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
    10201 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    10261 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
    10321 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
    10381 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    10441 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    10501 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    10561 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    10621 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    10681 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    10741 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    10801 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    10861 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    10921 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    10981 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    11041 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    11101 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    11161 gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc agcaaccagg
    11221 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag
    11281 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    11341 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    11401 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
    11461 aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg
    11521 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
    11581 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
    11641 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
    11701 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
    11761 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
    11821 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
    11881 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
    11941 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
    12001 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
    12061 cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa
    12121 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    12181 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    12241 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
    12301 tcgaggcctg caggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
    12361 atggaccttc taggtcttga aaggagtggg aatt
//
