LOCUS       sequence-353022-        8772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HA.NWS.VSVg_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        89..193
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(194..262,263..1054)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(412..431)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1225..1813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1714..1733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1967..1984
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2101..2122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2137..2142,2143..2160,2161..2167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2175..2191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2180..2202
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2199..2215
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2199..2215
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2234..2254
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2236..2254
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2282..2508
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2509..2689
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2736..2861
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3354..3587
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3772..3816
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4114..4362
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4114..4134
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4285..4304
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4381..4456
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4504..4621
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4761..5939
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4992..5930
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5887..5907
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5988..5997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6000..6026
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6048..6071
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6090..6122
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6132..6839
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6277..6295)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6545..6564
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6909..7497
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6962..6982)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7380..7391)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7572..7805
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7877..7998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7914..7933)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7968..7987
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8038..8173
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8193..8212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8194..8212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8222..8239)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8222..8238)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8231..8253)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8380..8772,1..63)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8467..8486)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8677..8698
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
       61 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      121 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      181 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      241 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      301 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      361 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      421 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      481 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      541 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      601 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      661 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      721 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
      781 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
      841 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      901 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      961 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1021 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1081 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1141 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1201 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1261 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1321 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1381 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1441 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1501 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1561 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1621 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1681 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1741 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1801 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1861 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1921 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1981 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     2041 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2101 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2161 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2221 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     2281 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     2341 gccttacaag gagagaaaaa gcaccgtgca tggcgattgg tggaagtaag gtggtacgat
     2401 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     2461 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     2521 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2581 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2641 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     2701 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     2761 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2821 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     2881 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     2941 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     3001 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     3061 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     3121 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     3181 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     3241 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     3301 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     3361 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     3421 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     3481 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     3541 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     3601 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     3661 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     3721 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     3781 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     3841 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     3901 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     3961 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     4021 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca
     4081 cgagactagt atcgagcggc cgcccccttc accgagggcc tatttcccat gattccttca
     4141 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
     4201 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     4261 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     4321 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc gagaagacct
     4381 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     4441 ggcaccgagt cggtgctttt tttaattctc gacctcgaga caaatggcag tattcatcca
     4501 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     4561 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     4621 tcgggtttat tacagggaca gcagagatcc actttggccg cggctcgagg cgataagctt
     4681 tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg
     4741 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     4801 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     4861 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     4921 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     4981 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     5041 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     5101 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     5161 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     5221 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     5281 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     5341 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     5401 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     5461 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     5521 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     5581 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     5641 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     5701 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     5761 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     5821 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     5881 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     5941 gaattctgca gtcgatcgac ggtaccgcgg gcccgggatc cacggtcgcc accatgggct
     6001 acccatacga tgtgccagat tacgctggag gaggcggggg agggaactgg tcccaccccc
     6061 aattcgagaa aggaggaggc gggggagggt atacagatat cgaaatgaat agactcggaa
     6121 agggcatgct gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     6181 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     6241 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     6301 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     6361 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     6421 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     6481 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     6541 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     6601 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     6661 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     6721 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     6781 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaaga
     6841 aacggccagc cgaggatatg gaggaagaac aagcctttaa acgatctcga taaagcggcc
     6901 gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6961 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7021 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7081 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7141 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7201 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7261 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     7321 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7381 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7441 cgcatcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     7501 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7561 aaagggggga ctggaagggc taattcactc ccaacgaaga taagatctgc tttttgcttg
     7621 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7681 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7741 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7801 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7861 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7921 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7981 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     8041 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8101 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8161 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     8221 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     8281 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     8341 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     8401 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8461 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8521 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8581 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8641 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8701 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8761 cctattggtt aa
//
