LOCUS       sequence-353024-       14053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14053)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2324
FEATURES             Location/Qualifiers
     source          1..14053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2769..2783)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     misc_feature    6991..7579
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7044..7064)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7462..7473)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7651..7884
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7962..8083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7999..8018)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8053..8072
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8123..8258
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8278..8297)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8279..8297)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8307..8324)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8307..8323)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8316..8338)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8465..8920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8552..8571)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8762..8783
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8946..9050
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9051..9119,9120..9911)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9269..9288)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10082..10670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10571..10590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10824..10841
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10958..10979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10994..10999,11000..11017,11018..11024)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11032..11048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11037..11059
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11056..11072
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11056..11072
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11091..11111
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11093..11111
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11139..11365
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11366..11546
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11593..11718
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12211..12444
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12629..12673
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        12904..13114
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        12926..13122
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12973..13108
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             13154..13756
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(13154..13173)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13650..13670
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13799..13916
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggg
     1201 acccggctgc gatttgctgc tgcggacagc agctaccatc actgctgccg ccatcatgtc
     1261 agacacggac agcgacgaag attccgctgg aggcggccca ttttctttag cgggtttcct
     1321 tttcggcaac atcaatggag ccgggcagct ggagggggaa agcgtcttgg atgatgaatg
     1381 taagaagcac ttggcaggct tgggggcttt ggggctgggc agcctgatca ctgaactcac
     1441 ggcaaatgaa gaattgaccg ggactgacgg tgccttggta aatgatgaag ggtgggttag
     1501 gagtacagaa gatgctgtgg actattcaga catcaatgag gtggcagaag atgaaagccg
     1561 aagataccag cagacgatgg ggagcttgca gcccctttgc cactcagatt atgatgaaga
     1621 tgactatgat gctgattgtg aagacattga ttgcaagttg atgcctcctc cacctccacc
     1681 cccgggacca atgaagaagg ataaggacca ggattctatt actggtgtgt ctgaaaatgg
     1741 agaaggcatc atcttgccct ccatcattgc cccttcctct ttggcctcag agaaagtgga
     1801 cttcagtagt tcctctgact cagaatctga gatgggacct caggaagcaa cacaggcaga
     1861 atctgaagat ggaaagctga cccttccatt ggctgggatt atgcagcatg atgccaccaa
     1921 gctgttgcca agtgtcacag aactttttcc agaatttcga cctggaaagg tgttacgttt
     1981 tctacgtctt tttggaccag ggaagaatgt cccatctgtt tggcggagtg ctcggagaaa
     2041 gaggaagaag aagcaccgtg agctgataca ggaagagcag atccaggagg tggagtgctc
     2101 agtagaatca gaagtcagcc agaagtcttt gtggaactac gactacgctc caccaccacc
     2161 tccagagcag tgtctctctg atgatgaaat cacgatgatg gctcctgtgg agtccaaatt
     2221 ttcccaatca actggagata tagataaagt gacagatacc aaaccaagag tggctgagtg
     2281 gcgttatggg cctgcccgac tgtggtatga tatgctgggt gtccctgaag atggcagtgg
     2341 gtttgactat ggcttcaaac tgagaaagac agaacatgaa cctgtgataa aatctagaat
     2401 gatagaggaa tttaggaaac ttgaggaaaa caatggcact gatcttctgg ctgatgaaaa
     2461 cttcctgatg gtgacacagc tgcattggga ggatgatatc atctgggatg gggaggatgt
     2521 caaacacaaa gggacaaaac ctcagcgtgc aagcctggca ggctggcttc cttctagcat
     2581 gactaggaat gcgatggctt acaatgttca gcaaggtttt gcagccactc ttgatgatga
     2641 caaaccttgg tactccattt ttcccattga caatgaggat ctggtatatg gacgctggga
     2701 ggacaatatc atttgggatg ctcaggccat gccccggctg ttggaacctc ctgttttgac
     2761 acttgatccc aatgatgaga acctcatttt ggaaattcct gatgagaagg aagaggccac
     2821 ctctaactcc ccctccaagg agagtaagaa ggaatcatct ctgaagaaga gtcgaattct
     2881 cttagggaaa acaggagtca tcaaggagga accacagcag aacatgtctc agccagaagt
     2941 gaaagatcca tggaatctct ccaatgatga gtattattat cccaagcaac agggtcttcg
     3001 aggcaccttt ggagggaata ttatccagca ttcaattcct gctgtggaat tacggcagcc
     3061 cttctttccc acccacatgg ggcccatcaa actccggcag ttccatcgcc cacctctgaa
     3121 aaagtactca tttggtgcac tttctcagcc aggtccccac tcagtccaac ctttgctaaa
     3181 gcacatcaaa aaaaaggcca agatgagaga acaagagagg caagcttcag gtggtggaga
     3241 gatgtttttt atgcgcacac ctcaggacct cacaggcaaa gatggtgatc ttattcttgc
     3301 agaatatagt gaggaaaatg gacccttaat gatgcaggtt ggcatggcaa ccaagataaa
     3361 gaactattat aaacggaaac ctggaaaaga tcctggagca ccagattgta aatatgggga
     3421 aactgtttac tgccatacat ctcctttcct gggttctctc catcctggcc aattgctgca
     3481 agcatttgag aacaaccttt ttcgtgctcc aatttatctt cataagatgc cagaaactga
     3541 tttcttgatc attcggacaa gacagggtta ctatattcgg gaattagtgg atatttttgt
     3601 ggttggccag cagtgtccct tgtttgaagt tcctgggcct aactccaaaa gggccaatac
     3661 gcatattcga gactttctac aggtttttat ttaccgcctt ttctggaaaa gtaaagatcg
     3721 gccacggagg atacgaatgg aagatataaa aaaagccttt ccttcccatt cagaaagcag
     3781 catccggaag aggctaaagc tctgcgctga cttcaaacgc acagggatgg actcaaactg
     3841 gtgggtgctt aagtctgatt ttcgtttacc aacggaagaa gagatcagag ctatggtgtc
     3901 accagagcag tgctgtgctt attatagcat gatagctgca gagcaacgac tgaaggatgc
     3961 tggctatggt gagaaatcct tttttgctcc agaagaagaa aatgaggaag atttccagat
     4021 gaagattgat gatgaagttc gcactgcccc ttggaacacc acaagggcct tcattgctgc
     4081 catgaagggc aagtgtctgc tagaggtgac tggggtggca gatcccacgg ggtgtggtga
     4141 aggattctcc tatgtgaaga ttccaaacaa accaacacag cagaaggatg ataaagaacc
     4201 gcagccagtg aagaagacag tgacaggaac agatgcagac cttcgtcgcc tttccctgaa
     4261 aaatgccaag caacttctac gtaaatttgg tgtgcctgag gaagagatta aaaagttgtc
     4321 ccgctgggaa gtgattgatg tggtgcgcac aatgtcaaca gaacaggctc gttctggaga
     4381 ggggcccatg agtaaatttg cccgtggatc aaggttttct gtggctgagc atcaagagcg
     4441 ttacaaagag gaatgtcagc gcatctttga cctacagaac aaggttctgt catcaactga
     4501 agtcttatca actgacacag acagcagctc agctgaagat agtgactttg aagaaatggg
     4561 aaagaacatt gagaacatgt tgcagaacaa gaaaaccagc tctcagcttt cacgtgaacg
     4621 ggaggaacag gagcggaagg aactacagcg aatgctactg gcagcaggct cagcagcatc
     4681 cggaaacaat cacagagatg atgacacagc ttccgtgact agccttaact cttctgccac
     4741 tggacgctgt ctcaagattt atcgcacgtt tcgagatgaa gaggggaaag agtatgttcg
     4801 ctgtgagaca gtccgaaaac cagctgtcat tgatgcctat gtgcgcatac ggactacaaa
     4861 agatgaggaa ttcattcgaa aatttgccct ttttgatgaa caacatcggg aagagatgcg
     4921 aaaagaacgg cggaggattc aagagcaact gaggcggctt aagaggaacc aggaaaagga
     4981 gaagcttaag ggtcctcctg agaagaagcc caagaaaatg aaggagcgtc ctgacctaaa
     5041 actgaaatgt ggggcatgtg gtgccattgg acacatgagg actaacaaat tctgccccct
     5101 ctattatcaa acaaatgcgc caccttccaa ccctgttgcc atgacagaag aacaggagga
     5161 ggagttggaa aagacagtca ttcataatga taatgaagaa cttatcaagg ttgaagggac
     5221 caaaattgtc ttggggaaac agctaattga gagtgcggat gaggttcgca gaaaatctct
     5281 ggttctcaag tttcctaaac agcagcttcc tccaaagaag aaacggcgag ttggaaccac
     5341 tgttcactgt gactatttga atagacctca taagtccatc caccggcgcc gcacagaccc
     5401 tatggtgacg ctgtcgtcca tcttggagtc tatcatcaat gacatgagag atcttccaaa
     5461 tacataccct ttccacactc cagtcaatgc aaaggttgta aaggactact acaaaatcat
     5521 cactcggcca atggacctac aaacactccg cgaaaacgtg cgtaaacgcc tctacccatc
     5581 tcgggaagag ttcagagagc atctggagct aattgtgaaa aatagtgcaa cctacaatgg
     5641 gccaaaacac tcattgactc agatctctca atccatgctg gatctctgtg atgaaaaact
     5701 caaagagaaa gaagacaaat tagctcgctt agagaaagct atcaacccct tgctggatga
     5761 tgatgaccaa gtggcgtttt ctttcattct ggacaacatt gtcacccaga aaatgatggc
     5821 agttccagat tcttggccat ttcatcaccc agttaataag aaatttgttc cagattatta
     5881 caaagtgatt gtcaatccaa tggatttaga gaccatacgt aagaacatct ccaagcacaa
     5941 gtatcagagt cgggagagct ttctggatga tgtaaacctt attctggcca acagtgttaa
     6001 gtataatgga cctgagagtc agtatactaa gactgcccag gagattgtga acgtctgtta
     6061 ccagacattg actgagtatg atgaacattt gactcaactt gagaaggata tttgtactgc
     6121 taaagaagca gctttggagg aagcagaatt agaaagcctg gacccaatga ccccagggcc
     6181 ctacacgcct caggctaagc ctcctgattt gtatgatacc aacacatccc tcagtatgtc
     6241 tcgagatgcc tctgtatttc aagatgagag caatatgtct gtcttggata ttcccagtgc
     6301 cactccagaa aagcaggtaa cacaggaagg tgaagatgga gatggtgatc ttgcagatga
     6361 agaggaagga actgtacaac agcctcaagc cagtgtcctg tatgaggatt tgcttatgtc
     6421 tgaaggagaa gatgatgagg aagatgctgg gagtgatgaa gaaggagaca atcctttctc
     6481 tgctatccag ctgagtgaaa gtggaagtga ctctgatgtg ggatctggtg gaataagacc
     6541 caaacaaccc cgcatgcttc aggagaacac aaggatggac atggaaaatg aagaaagcat
     6601 gatgtcctat gagggagacg gtggggaggc ttcccatggt ttggaggata gcaacatcag
     6661 ttatgggagc tatgaggagc ctgatcccaa gtcgaacacc caagacacaa gcttcagcag
     6721 catcggtggg tatgaggtat cagaggagga agaagatgag gaggaggaag agcagcgctc
     6781 tgggccgagc gtactaagcc aggtccacct gtcagaggac gaggaggaca gtgaggattt
     6841 ccactccatt gctggggaca gtgacttgga ctctgatgaa tagtaatgaa ctagtccgta
     6901 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacttattt
     6961 atactagcag aggtcacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     7021 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7081 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7141 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7201 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7261 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7321 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7381 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7441 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7501 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7561 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     7621 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7681 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7741 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7801 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7861 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     7921 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7981 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8041 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     8101 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8161 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8221 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     8281 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     8341 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     8401 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     8461 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8521 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8581 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8641 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8701 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8761 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8821 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8881 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     8941 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9001 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     9061 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9121 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     9181 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     9241 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     9301 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     9361 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9421 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     9481 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9541 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9601 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9661 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9721 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9781 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9841 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9901 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9961 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    10021 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10081 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10141 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    10201 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    10261 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    10321 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    10381 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    10441 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    10501 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10561 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10621 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10681 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10741 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    10801 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    10861 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    10921 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    10981 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    11041 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    11101 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
    11161 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
    11221 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
    11281 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
    11341 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
    11401 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    11461 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    11521 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    11581 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    11641 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    11701 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    11761 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    11821 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    11881 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    11941 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    12001 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    12061 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
    12121 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
    12181 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
    12241 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
    12301 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
    12361 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
    12421 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
    12481 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
    12541 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
    12601 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
    12661 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
    12721 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
    12781 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
    12841 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    12901 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
    12961 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    13021 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    13081 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
    13141 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
    13201 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
    13261 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
    13321 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
    13381 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
    13441 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
    13501 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
    13561 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
    13621 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
    13681 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
    13741 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
    13801 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
    13861 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
    13921 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
    13981 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
    14041 aggagtggga att
//
