LOCUS       sequence-353035-       12641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus 
            Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a 
            mutation in the PAM site used by POLBKO gRNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12641)
  AUTHORS   Koczor CA, Saville KM, Andrews JF, Clark J, Fang Q, Li J, 
            Al-Rahahleh RQ, Ibrahim M, McClellan S, Makarov MV, Migaud ME, Sobol
            RW
  TITLE     Temporal dynamics of base excision/single-strand break repair 
            protein complex assembly/disassembly are modulated by the 
            PARP/NAD(+)/SIRT6 axis.
  JOURNAL   Cell Rep. 2021 Nov 2;37(5):109917. doi: 
            10.1016/j.celrep.2021.109917.
  PUBMED    34731617
REFERENCE   2  (bases 1 to 12641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-11-2"; volume: "37(5):109917. doi"; pages: " 
            10.1016/j.celrep.2021.109917"
COMMENT     Sequence Label: 
            pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)
FEATURES             Location/Qualifiers
     source          1..12641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..3137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2190..3128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3085..3105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3137..3161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(5067..5069,5070..5072,5073..5783)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5067..5069,5070..5072,5073..5783)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5112..5133)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5373..5392)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5720..5741
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5793..5846
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5850..5879
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(6888..6912)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6930..7518
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6983..7003)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7401..7412)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7549..8048
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7584..7607)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7981..8000
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8065..9090
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8065..9090
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             9162..9395
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9467..9588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9504..9523)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9558..9577
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9628..9763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9783..9802)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9784..9802)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9812..9829)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9812..9828)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9821..9843)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9970..10425
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10057..10076)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10267..10288
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10451..10555
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10556..10624,10625..11416)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10774..10793)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11587..12175
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12076..12095
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12329..12346
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12463..12484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12499..12504,12505..12522,12523..12529)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12537..12553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12542..12564
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12561..12577
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12561..12577
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12596..12616
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12598..12616
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg ccggagatcc
     3181 gcctccgcca tgtcgtgtcc tgcagcagcc aggactcgac tcactgtgca gaaaatcttc
     3241 tcaaggcaga cacttaccga aaatggcggg cagccaaggc aggcgagaag accatctctg
     3301 tggtcctaca gttggagaag gaggagcaga tacacagtgt ggacattggg aatgatggct
     3361 cagctttcgt ggaggtgctg gtgggcagtt cagctggagg cgctggggag caagactatg
     3421 aggtccttct ggtcacctca tctttcatgt ccccttccga gagccgcagt ggctcaaacc
     3481 ccaaccgcgt tcgcatgttt gggcctgaca agctggtccg ggcagccgcc gagaagcgct
     3541 gggaccgggt caaaattgtt tgcagccagc cctacagcaa ggactccccc tttggcttga
     3601 gttttgtacg gtttcatagc cccccagaca aagatgaggc agaggccccg tcccagaagg
     3661 tgacagtgac caagcttggc cagttccgtg tgaaggagga ggatgagagc gccaactctc
     3721 tgaggccggg ggctctcttc ttcagccgga tcaacaagac atccccagtc acagccagcg
     3781 acccagcagg acctagctat gcagctgcta ccctccaggc ttctagtgct gcctcctcag
     3841 cctctccagt ctccagggcc ataggcagca cctccaagcc ccaggagtct cccaaaggga
     3901 agaggaagtt ggatttgaac caagaagaaa agaagacccc cagcaaacca ccagcccagc
     3961 tgtcgccatc tgttcccaag agacctaaat tgccagctcc aactcgtacc ccagccacag
     4021 ccccagtccc tgcccgagca cagggggcag tgacaggcaa accccgagga gaaggcaccg
     4081 agcccagacg accccgagct ggcccagagg agctggggaa gatccttcag ggtgtggtag
     4141 tggtgctgag tggcttccag aaccccttcc gctccgagct gcgagataag gccctagagc
     4201 ttggggccaa gtatcggcca gactggaccc gggacagcac gcacctcatc tgtgcctttg
     4261 ccaacacccc caagtacagc caggtcctag gcctgggagg ccgcatcgtg cgtaaggagt
     4321 gggtgctgga ctgtcaccgc atgcgtcggc ggctgccctc ccagaggtac ctcatggcag
     4381 ggccaggttc cagcagtgag gaggatgagg cctctcacag cggtggcagc ggagatgaag
     4441 cccccaagct tcctcagaag caaccccaga ccaaaaccaa gcccactcag gcagctggac
     4501 ccagctcacc ccagaagccc ccaacccctg aagagaccaa agcagcctca ccagtgctcc
     4561 aggaagatat agacattgag ggggtacagt cagaaggaca ggacaatggg gcggaagatt
     4621 ctggggacac agaggatgag ctgaggaggg tggcagagca gaaggaacac agactgcccc
     4681 ctggccagga ggagaatggg gaagacccgt atgcaggctc cacggatgag aacacggaca
     4741 gtgaggaaca ccaggagcct cctgatctgc cagtccctga gctcccagat ttcttccagg
     4801 gcaagcactt ctttctttac ggggagttcc ctggggacga gcggcggaaa ctcatccgat
     4861 acgtcacagc cttcaatggg gagctcgagg actatatgag tgaccgggtt cagtttgtga
     4921 tcacagcaca ggaatgggat cccagctttg aggaggccct gatggacaac ccctccctgg
     4981 cattcgttcg tccccgatgg atctacagtt gcaatgagaa gcagaagtta cttcctcacc
     5041 agctctatgg ggtggtgccg caagccatgg tgagcaaggg cgaggagctg ttcaccgggg
     5101 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     5161 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     5221 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     5281 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     5341 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     5401 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     5461 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     5521 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     5581 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5641 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     5701 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5761 tcggcatgga cgagctgtac aagggaagcg gagagggcag gggaagtctt ctaacatgcg
     5821 gggacgtgga ggaaaatccc ggccccatgg aacaaaaact catctcagaa gaggatctga
     5881 tgagcaaacg gaaggcgccg caagagactc tcaacggggg aatcaccgac atgctcacag
     5941 aactcgcaaa ctttgagaag aacgtgagcc aagctatcca cgcgtacaat gcttacagaa
     6001 aagcagcatc tgttatagca aaatacccac acaaaataaa gagtggagct gaagctaaga
     6061 aattgcctgg agtaggaaca gcgattgctg aagcgattga tgagttttta gcaactggaa
     6121 aattacgtaa actggaaaag attcggcagg atgatacgag ttcatccatc aatttcctga
     6181 ctcgagttag tggcattggt ccatctgctg caaggaagtt tgtagatgaa ggaattaaaa
     6241 cactagaaga tctcagaaaa aatgaagata aattgaacca tcatcagcga attgggctga
     6301 aatattttgg ggactttgaa aaaagaattc ctcgtgaaga gatgttacaa atgcaagata
     6361 ttgtactaaa tgaagttaaa aaagtggatt ctgaatacat tgctacagtc tgtggcagtt
     6421 tcagaagagg tgcagagtcc agtggtgaca tggatgttct cctgacccat cccagcttca
     6481 cttcagaatc aaccaaacag ccaaaactgt tacatcaggt tgtggagcag ttacaaaagg
     6541 ttcattttat cacagatacc ctgtcaaagg gtgagacaaa gttcatgggt gtttgccagc
     6601 ttcccagtaa aaatgatgaa aaagaatatc cacacagaag aattgatatc aggttgatac
     6661 ccaaagatca gtattactgt ggtgttctct atttcactgg gagtgatatt ttcaataaga
     6721 atatgagggc tcatgcccta gaaaagggtt tcacaatcaa tgagtacacc atccgtccct
     6781 tgggagtcac tggagttgca ggagaacccc tgccagtgga tagtgaaaaa gacatctttg
     6841 attacatcca gtggaaatac cgggaaccca aggaccggag cgaatgaacc cagctttctt
     6901 gtacaaagtg gtgataatcg aattccgata atcaacctct ggattacaaa atttgtgaaa
     6961 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7021 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7081 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7141 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7201 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7261 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7321 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7381 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7441 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7501 tttgggccgc ctccccgcat cgggaattcc cgcggttcga attctaccgg gtaggggagg
     7561 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     7621 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     7681 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     7741 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     7801 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     7861 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     7921 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     7981 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     8041 gcctttcgac ctcacgtggc caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     8101 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     8161 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     8221 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     8281 ccgattccgg aagtgcttga cattggggaa tttagcgaga gcctgaccta ttgcatctcc
     8341 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     8401 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     8461 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     8521 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     8581 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     8641 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     8701 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     8761 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     8821 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     8881 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     8941 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     9001 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     9061 cccagcactc gtccgagggc aaaggaatag ggtaccttta agaccaatga cttacaaggc
     9121 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     9181 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     9241 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9301 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9361 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     9421 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     9481 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9541 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     9601 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9661 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9721 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     9781 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     9841 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     9901 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     9961 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    10021 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    10081 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    10141 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    10201 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    10261 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    10321 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    10381 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
    10441 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    10501 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    10561 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    10621 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    10681 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    10741 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    10801 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    10861 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    10921 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    10981 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    11041 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    11101 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    11161 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    11221 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    11281 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    11341 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    11401 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    11461 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    11521 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    11581 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    11641 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    11701 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    11761 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    11821 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    11881 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    11941 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    12001 cgaagagaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    12061 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    12121 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    12181 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    12241 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    12301 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    12361 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    12421 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    12481 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    12541 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
    12601 taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
