LOCUS       sequence-353145-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_NWS.Ollas.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        73..177
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(178..246,247..1038)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(396..415)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1209..1797
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1698..1717
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1951..1968
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2085..2106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2121..2126,2127..2144,2145..2151)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2159..2175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2164..2186
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2183..2199
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2183..2199
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2218..2238
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2220..2238
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2266..2492
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2493..2673
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2720..2845
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3338..3571
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3756..3800
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4098..4346
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4098..4118
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4269..4288
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4365..4440
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4488..4605
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4745..5923
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4976..5914
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5871..5891
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5972..5981
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5987..6010
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6029..6070
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6092..6133
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6143..6850
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6288..6306)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6556..6575
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6920..7508
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6973..6993)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7391..7402)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7583..7816
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7888..8009
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7925..7944)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7979..7998
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8049..8184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8204..8223)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8205..8223)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8233..8250)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8233..8249)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8242..8264)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8391..8799,1..47)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8478..8497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8688..8709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
       61 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      121 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      181 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      241 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      301 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      361 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      421 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      481 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      541 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      601 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      661 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
      721 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
      781 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      841 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      901 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      961 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1021 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1081 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1141 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1201 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1261 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1321 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1381 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1441 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1501 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1561 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1621 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1681 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1741 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1801 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1861 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1921 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1981 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2041 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     2101 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     2161 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     2221 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     2281 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     2341 aaaagcaccg tgcatggcga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     2401 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     2461 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     2521 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     2581 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     2641 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     2701 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     2761 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     2821 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     2881 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     2941 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     3001 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     3061 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     3121 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     3181 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     3241 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     3301 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     3361 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     3421 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     3481 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     3541 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     3601 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     3661 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     3721 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     3781 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     3841 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     3901 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     3961 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     4021 acagatccat tcgattagtg aacggatctc gacggtatcg atcacgagac tagtatcgag
     4081 cggccgcccc cttcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat
     4141 acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
     4201 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     4261 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     4321 atatcttgtg gaaaggacga aacaccgggt cttcgagaag acctgtttta gagctagaaa
     4381 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     4441 tttttttaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa
     4501 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     4561 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     4621 gacagcagag atccactttg gccgcggctc gaggcgataa gctttgcaaa gatggataaa
     4681 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     4741 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     4801 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     4861 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     4921 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     4981 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     5041 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     5101 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     5161 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     5221 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     5281 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     5341 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     5401 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5461 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5521 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     5581 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5641 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5701 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5761 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5821 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5881 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaattc tgcagtcgat
     5941 cgacggtacc gcgggcccgg gatccacggt cgccaccatg ggcaactggt cccaccccca
     6001 attcgagaaa ggaggaggcg ggggagggag cggcttcgcc aacgagctgg gccccaggct
     6061 gatgggcaag tgcggaggag gcgggggagg gggcaaacca attccaaacc cactgctggg
     6121 actggattcc acaggcatgc tgatggtgag caagggcgag gaggataaca tggccatcat
     6181 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     6241 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     6301 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     6361 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     6421 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     6481 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     6541 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     6601 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     6661 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     6721 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     6781 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     6841 gctgtacaag aaacggccag ccgaggatat ggaggaagaa caagccttta aacgatctcg
     6901 ataaagcggc cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6961 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7021 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7081 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7141 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7201 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7261 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7321 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7381 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7441 gcggcctctt ccgcatcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7501 ctccccgcct gggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7561 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag ataagatctg
     7621 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7681 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7741 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7801 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     7861 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7921 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7981 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     8041 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8101 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8161 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     8221 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8281 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8341 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     8401 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8461 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8521 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8581 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8641 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8701 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8761 gccgatttcg gcctattggt taaaaaatga gctgattta
//
