LOCUS       sequence-353178-       12436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12436)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1995
FEATURES             Location/Qualifiers
     source          1..12436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5374..5962
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5427..5447)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5845..5856)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6034..6267
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6345..6466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6382..6401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6436..6455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6506..6641
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6661..6680)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6662..6680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6690..6707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6690..6706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6699..6721)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6848..7303
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6935..6954)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7145..7166
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7329..7433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7434..7502,7503..8294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7652..7671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8465..9053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8954..8973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9207..9224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9341..9362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9377..9382,9383..9400,9401..9407)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9415..9431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9420..9442
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9439..9455
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9439..9455
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9474..9494
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9476..9494
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9522..9748
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9749..9929
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9976..10101
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10594..10827
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11012..11056
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11287..11497
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11309..11505
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11356..11491
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11537..12139
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11537..11556)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12033..12053
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12182..12299
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgca
     1201 gagcaaggtg ctgctggccg tcgccctgtg gctctgcgtg gagacccggg ccgcctctgt
     1261 gggtttgcct agtgtttctc ttgatctgcc caggctcagc atacaaaaag acatacttac
     1321 aattaaggct aatacaactc ttcaaattac ttgcagggga cagagggact tggactggct
     1381 ttggcccaat aatcagagtg gcagtgagca aagggtggag gtgactgagt gcagcgatgg
     1441 cctcttctgt aagacactca caattccaaa agtgatcgga aatgacactg gagcctacaa
     1501 gtgcttctac cgggaaactg acttggcctc ggtcatttat gtctatgttc aagattacag
     1561 atctccattt attgcttctg ttagtgacca acatggagtc gtgtacatta ctgagaacaa
     1621 aaacaaaact gtggtgattc catgtctcgg gtccatttca aatctcaacg tgtcactttg
     1681 tgcaagatac ccagaaaaga gatttgttcc tgatggtaac agaatttcct gggacagcaa
     1741 gaagggcttt actattccca gctacatgat cagctatgct ggcatggtct tctgtgaagc
     1801 aaaaattaat gatgaaagtt accagtctat tatgtacata gttgtcgttg tagggtatag
     1861 gatttatgat gtggttctga gtccgtctca tggaattgaa ctatctgttg gagaaaagct
     1921 tgtcttaaat tgtacagcaa gaactgaact aaatgtgggg attgacttca actgggaata
     1981 cccttcttcg aagcatcagc ataagaaact tgtaaaccga gacctaaaaa cccagtctgg
     2041 gagtgagatg aagaaatttt tgagcacctt aactatagat ggtgtaaccc ggagtgacca
     2101 aggattgtac acctgtgcag catccagtgg gctgatgacc aagaagaaca gcacatttgt
     2161 cagggtccat gaaaaacctt ttgttgcttt tggaagtggc atggaatctc tggtggaagc
     2221 cacggtgggg gagcgtgtca gaatccctgc gaagtacctt ggttacccac ccccagaaat
     2281 aaaatggtat aaaaatggaa taccccttga gtccaatcac acaattaaag cggggcatgt
     2341 actgacgatt atggaagtga gtgaaagaga cacaggaaat tacactgtca tccttaccaa
     2401 tcccatttca aaggagaagc agagccatgt ggtctctctg gttgtgtatg tcccacccca
     2461 gattggtgag aaatctctaa tctctcctgt ggattcctac cagtacggca ccactcaaac
     2521 gctgacatgt acggtctatg ccattcctcc cccgcatcac atccactggt attggcagtt
     2581 ggaggaagag tgcgccaacg agcccagcca agctgtctca gtgacaaacc catacccttg
     2641 tgaagaatgg agaagtgtgg aggacttcca gggaggaaat aaaattgaag ttaataaaaa
     2701 tcaatttgct ctaattgaag gaaaaaacaa aactgtaagt acccttgtta tccaagcggc
     2761 aaatgtgtca gctttgtaca aatgtgaagc ggtcaacaaa gtcgggagag gagagagggt
     2821 gatctccttc cacgtgacca ggggtcctga aattactttg caacctgaca tgcagcccac
     2881 tgagcaggag agcgtgtctt tgtggtgcac tgcagacaga tctacgtttg agaacctcac
     2941 atggtacaag cttggcccac agcctctgcc aatccatgtg ggagagttgc ccacacctgt
     3001 ttgcaagaac ttggatactc tttggaaatt gaatgccacc atgttctcta atagcacaaa
     3061 tgacattttg atcatggagc ttaagaatgc atccttgcag gaccaaggag actatgtctg
     3121 ccttgctcaa gacaggaaga ccaagaaaag acattgcgtg gtcaggcagc tcacagtcct
     3181 agagcgtgtg gcacccacga tcacaggaaa cctggagaat cagacgacaa gtattgggga
     3241 aagcatcgaa gtctcatgca cggcatctgg gaatccccct ccacagatca tgtggtttaa
     3301 agataatgag acccttgtag aagactcagg cattgtattg aaggatggga accggaacct
     3361 cactatccgc agagtgagga aggaggacga aggcctctac acctgccagg catgcagtgt
     3421 tcttggctgt gcaaaagtgg aggcattttt cataatagaa ggtgcccagg aaaagacgaa
     3481 cttggaaatc attattctag taggcacggc ggtgattgcc atgttcttct ggctacttct
     3541 tgtcatcatc ctacggaccg ttaagcgggc caatggaggg gaactgaaga caggctactt
     3601 gtccatcgtc atggatccag atgaactccc attggatgaa cattgtgaac gactgcctta
     3661 tgatgccagc aaatgggaat tccccagaga ccggctgaag ctaggtaagc ctcttggccg
     3721 tggtgccttt ggccaagtga ttgaagcaga tgcctttgga attgacaaga cagcaacttg
     3781 caggacagta gcagtcaaaa tgttgaaaga aggagcaaca cacagtgagc atcgagctct
     3841 catgtctgaa ctcaagatcc tcattcatat tggtcaccat ctcaatgtgg tcaaccttct
     3901 aggtgcctgt accaagccag gagggccact catggtgatt gtggaattct gcaaatttgg
     3961 aaacctgtcc acttacctga ggagcaagag aaatgaattt gtcccctaca agaccaaagg
     4021 ggcacgattc cgtcaaggga aagactacgt tggagcaatc cctgtggatc tgaaacggcg
     4081 cttggacagc atcaccagta gccagagctc agccagctct ggatttgtgg aggagaagtc
     4141 cctcagtgat gtagaagaag aggaagctcc tgaagatctg tataaggact tcctgacctt
     4201 ggagcatctc atctgttaca gcttccaagt ggctaagggc atggagttct tggcatcgcg
     4261 aaagtgtatc cacagggacc tggcggcacg aaatatcctc ttatcggaga agaacgtggt
     4321 taaaatctgt gactttggct tggcccggga tatttataaa gatccagatt atgtcagaaa
     4381 aggagatgct cgcctccctt tgaaatggat ggccccagaa acaatttttg acagagtgta
     4441 cacaatccag agtgacgtct ggtcttttgg tgttttgctg tgggaaatat tttccttagg
     4501 tgcttctcca tatcctgggg taaagattga tgaagaattt tgtaggcgat tgaaagaagg
     4561 aactagaatg agggcccctg attatactac accagaaatg taccagacca tgctggactg
     4621 ctggcacggg gagcccagtc agagacccac gttttcagag ttggtggaac atttgggaaa
     4681 tctcttgcaa gctaatgctc agcaggatgg caaagactac attgttcttc cgatatcaga
     4741 gactttgagc atggaagagg attctggact ctctctgcct acctcacctg tttcctgtat
     4801 ggaggaggag gaagtatgtg accccaaatt ccattatgac aacacagcag gaatcagtca
     4861 gtatctgcag aacagtaagc gaaagagccg gcctgtgagt gtaaaaacat ttgaagatat
     4921 cccgttagaa gaaccagaag taaaagtaat cccagatgac aaccagacgg acagtggtat
     4981 ggttcttgcc tcagaagagc tgaaaacttt ggaagacaga accaaattat ctccatcttt
     5041 tggtggaatg gtgcccagca aaagcaggga gtctgtggca tctgaaggct caaaccagac
     5101 aagcggctac cagtccggat atcactccga tgacacagac accaccgtgt actccagtga
     5161 ggaagcagaa cttttaaagc tgatagagat tggagtgcaa accggtagca cagcccagat
     5221 tctccagcct gactcgggga ccacactgag ctctcctcct gtttagtaat gaactagtcc
     5281 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaccagg
     5341 tatttgtatc agaacctgac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     5401 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5461 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5521 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5581 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5641 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5701 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5761 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     5821 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5881 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5941 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     6001 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6061 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6121 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6181 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6241 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     6301 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     6361 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6421 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     6481 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6541 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6601 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     6661 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     6721 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6781 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6841 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6901 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6961 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7021 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7081 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7141 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7201 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7261 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     7321 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7381 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7441 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7501 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7561 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7621 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7681 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7741 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7801 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7861 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7921 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7981 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8041 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8101 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8161 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8221 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8281 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8341 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8401 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8461 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8521 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8581 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8641 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8701 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8761 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8821 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8881 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8941 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9001 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9061 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9121 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9181 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9241 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9301 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9361 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9421 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     9481 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     9541 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     9601 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     9661 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     9721 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     9781 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9841 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9901 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     9961 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    10021 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    10081 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    10141 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    10201 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    10261 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    10321 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    10381 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    10441 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    10501 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    10561 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    10621 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    10681 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    10741 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    10801 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    10861 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    10921 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    10981 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    11041 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    11101 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    11161 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    11221 gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
    11281 tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg
    11341 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    11401 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
    11461 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt
    11521 accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
    11581 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
    11641 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
    11701 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
    11761 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
    11821 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
    11881 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
    11941 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
    12001 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
    12061 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
    12121 caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca
    12181 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
    12241 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
    12301 gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca
    12361 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
    12421 gaaaggagtg ggaatt
//
