LOCUS       sequence-353190-       10264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Koczor CA, Saville KM, Andrews JF, Clark J, Fang Q, Li J, 
            Al-Rahahleh RQ, Ibrahim M, McClellan S, Makarov MV, Migaud ME, Sobol
            RW
  TITLE     Temporal dynamics of base excision/single-strand break repair 
            protein complex assembly/disassembly are modulated by the 
            PARP/NAD(+)/SIRT6 axis.
  JOURNAL   Cell Rep. 2021 Nov 2;37(5):109917. doi: 
            10.1016/j.celrep.2021.109917.
  PUBMED    34731617
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-11-2"; volume: "37(5):109917. doi"; pages: " 
            10.1016/j.celrep.2021.109917"
COMMENT     Sequence Label: pLVX-CMV-XRCC1-gRNA res-Neo
FEATURES             Location/Qualifiers
     source          1..10264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2823..2847
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4782..4806)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(4838..5412,5413..5415,5416..5424)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5005..5022)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5232..5251
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5425..6219
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5479..5498)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6089..6108
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6233..6821
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6286..6306)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6704..6715)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7028..7661
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7789..7805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7789..7805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7802..7824)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7813..7829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7837..7843,7844..7861,7862..7867))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7882..7903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8020..8037)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8191..8779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8271..8290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8950..9741,9742..9810))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9573..9592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9811..9915)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9963..10097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10000..10019)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10054..10073
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactaat
     2821 caacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg gccccggaga
     2881 tccgcctccg ccatgtcgtg tcctgcagca gccaggactc gactcactgt gcagaaaatc
     2941 ttctcaaagc agacacttac cgaaaatggc gggcagccaa ggcaggcgag aagaccatct
     3001 ctgtggtcct acagttggag aaggaggagc agatacacag tgtagatata ggtaacgatg
     3061 gctcagcttt cgtggaggtg ctggtgggca gttcagctgg aggcgctggg gagcaagact
     3121 atgaggtcct tctggtcacc tcatctttca tgtccccttc cgagagccgc agtggctcaa
     3181 accccaaccg cgttcgcatg tttgggcctg acaagctggt ccgggcagcc gccgagaagc
     3241 gctgggaccg ggtcaaaatt gtttgcagcc agccctacag caaggactcc ccctttggct
     3301 tgagttttgt acggtttcat agccccccag acaaagatga ggcagaggcc ccgtcccaga
     3361 aggtgacagt gaccaagctt ggccagttcc gtgtgaagga ggaggatgag agcgccaact
     3421 ctctgaggcc gggggctctc ttcttcagcc ggatcaacaa gacatcccca gtcacagcca
     3481 gcgacccagc aggacctagc tatgcagctg ctaccctcca ggcttctagt gctgcctcct
     3541 cagcctctcc agtctccagg gccataggca gcacctccaa gccccaggag tctcccaaag
     3601 ggaagaggaa gttggatttg aaccaagaag aaaagaagac ccccagcaaa ccaccagccc
     3661 agctgtcgcc atctgttccc aagagaccta aattgccagc tccaactcgt accccagcca
     3721 cagccccagt ccctgcccga gcacaggggg cagtgacagg caaaccccga ggagaaggca
     3781 ccgagcccag acgaccccga gctggcccag aggagctggg gaagatcctt cagggtgtgg
     3841 tagtggtgct gagtggcttc cagaacccct tccgctccga gctgcgagat aaggccctag
     3901 agcttggggc caagtatcgg ccagactgga cccgggacag cacgcacctc atctgtgcct
     3961 ttgccaacac ccccaagtac agccaggtcc taggcctggg aggccgcatc gtgcgtaagg
     4021 agtgggtgct ggactgtcac cgcatgcgtc ggcggctgcc ctcccagagg tacctcatgg
     4081 cagggccagg ttccagcagt gaggaggatg aggcctctca cagcggtggc agcggagatg
     4141 aagcccccaa gcttcctcag aagcaacccc agaccaaaac caagcccact caggcagctg
     4201 gacccagctc accccagaag cccccaaccc ctgaagagac caaagcagcc tcaccagtgc
     4261 tccaggaaga tatagacatt gagggggtac agtcagaagg acaggacaat ggggcggaag
     4321 attctgggga cacagaggat gagctgagga gggtggcaga gcagaaggaa cacagactgc
     4381 cccctggcca ggaggagaat ggggaagacc cgtatgcagg ctccacggat gagaacacgg
     4441 acagtgagga acaccaggag cctcctgatc tgccagtccc tgagctccca gatttcttcc
     4501 agggcaagca cttctttctt tacggggagt tccctgggga cgagcggcgg aaactcatcc
     4561 gatacgtcac agccttcaat ggggagctcg aggactatat gagtgaccgg gttcagtttg
     4621 tgatcacagc acaggaatgg gatcccagct ttgaggaggc cctgatggac aacccctccc
     4681 tggcattcgt tcgtccccga tggatctaca gttgcaatga gaagcagaag ttacttcctc
     4741 accagctcta tggggtggtg ccgcaagcct gaggcgcgcc gacccagctt tcttgtacaa
     4801 agtggttgat ctagttctag agcggccgcg gatcccgccc ctctccctcc ccccccccta
     4861 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     4921 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     4981 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     5041 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     5101 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     5161 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     5221 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     5281 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     5341 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     5401 cacgatgata agcttgccac aaccatggct gaacaagatg gattgcacgc aggttctccg
     5461 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5521 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5581 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     5641 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5701 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5761 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5821 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5881 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5941 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6001 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6061 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6121 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6181 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa cgcgtctgga acaatcaacc
     6241 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6301 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6361 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6421 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6481 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6541 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6601 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     6661 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6721 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6781 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     6841 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6901 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     6961 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7021 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     7081 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     7141 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     7201 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     7261 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     7321 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     7381 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     7441 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     7501 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7561 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7621 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7681 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     7741 ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7801 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7861 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7921 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7981 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8041 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8101 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8161 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8221 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8281 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8341 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8401 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8461 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8521 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8581 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8641 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8701 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8761 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8941 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9001 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9061 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9121 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9181 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9241 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9301 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9361 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9421 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9481 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9541 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9601 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9661 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9721 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9781 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9841 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9901 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     9961 tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10021 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10081 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    10141 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    10201 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10261 tagt
//
