LOCUS       sequence-353285-        9883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Egr2 AS-RNA lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Martinez-Moreno M, O'Shea TM, Zepecki JP, Olaru A, Ness JK, Langer 
            R, Tapinos N
  TITLE     Regulation of Peripheral Myelination through Transcriptional 
            Buffering of Egr2 by an Antisense Long Non-coding RNA.
  JOURNAL   Cell Rep. 2017 Aug 22;20(8):1950-1963. doi: 
            10.1016/j.celrep.2017.07.068.
  PUBMED    28834756
REFERENCE   2  (bases 1 to 9883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-22- 22"; volume: "20"; issue: "8"; pages: "1950-1963"
COMMENT     Sequence Label: pLVX-Egr2ASRNA
FEATURES             Location/Qualifiers
     source          1..9883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             154..753
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(154..173)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     650..670
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    767..1355
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(820..840)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1238..1249)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1562..2195
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2324..2340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2324..2340)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2337..2359)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2348..2364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2372..2378,2379..2396,2397..2402))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2417..2438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2555..2572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2726..3314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2806..2825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3485..4276,4277..4345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4108..4127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4346..4450)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4498..4632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4535..4554)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4589..4608
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4800..5433
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5480..5605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6102..6335
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6520..6564
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6827..6942
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        7000..7303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7304..7507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7458..7478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7504..7528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    7610..7666
                     /label=MCS
                     /note="multiple cloning site"
     CDS             8645..9322
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(8825..8845)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     9043..9062
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9237..9260
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        9350..9849
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9385..9408)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gssgggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
       61 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
      121 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
      181 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
      241 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
      301 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
      361 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
      421 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
      481 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
      541 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
      601 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
      661 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
      721 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga
      781 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
      841 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
      901 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
      961 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     1021 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     1081 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     1141 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     1201 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     1261 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     1321 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     1381 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     1441 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     1501 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     1561 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     1621 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     1681 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     1741 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     1801 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     1861 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     1921 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     1981 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     2041 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     2101 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     2161 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     2221 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
     2281 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     2341 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2401 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2461 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2521 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2581 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2641 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3061 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3421 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3481 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3541 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3601 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3661 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3721 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3781 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3841 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3901 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3961 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4021 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4081 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4141 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4201 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4261 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4321 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4381 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4441 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac
     4501 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4561 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4621 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     4681 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     4741 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     4801 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     4861 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     4921 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     4981 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     5041 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     5101 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     5161 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     5221 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     5281 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5341 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5401 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     5461 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     5521 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     5581 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     5641 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     5701 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     5761 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     5821 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     5881 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     5941 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     6001 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     6061 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     6121 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     6181 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     6241 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     6301 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     6361 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     6421 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     6481 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     6541 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     6601 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     6661 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     6721 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     6781 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     6841 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     6901 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     6961 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     7021 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     7081 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     7141 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     7201 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     7261 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     7321 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     7381 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     7441 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     7501 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     7561 ccatagaaga caccgactct actagaggat cgctagcgct accggactca gatctcgagc
     7621 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatcctgct ctctcagacg
     7681 gaaagtgtta ggctcggagt atttatgggc aggtcttcaa taacccgtga cgtcgctgcc
     7741 catatatgga ctgaggaaca gggctgggcc tggtagcttt tgccggtcac atggccaatt
     7801 tgcatacggg cttggctgcg cgggctccgc cgagctcgcc cggaacgatt cgctgccggt
     7861 ggacaaccgc ccgggtctgg agctctcggc ccctgcctgc aacggggact ccggactgca
     7921 gagttcagcg cggggaaggc gcgatcaccg accctgacgc ggctgccgtg ggtgaaccca
     7981 cgggcagcga tggtagctct gtctcttggg gcggggagga attccggttc tccgagactt
     8041 tccaaaaaag gagaagatcc ggtagtggcg gctccgcctc cccagccctt gtttgggagc
     8101 cattccggaa aattaaactt cggaaagaaa ccgagcgctg ccgagacact acagtcaaac
     8161 ccctccttca ctttcttggc agctcaaaac cgcaagcaaa agaaaaaaaa aaaagacaga
     8221 gaaaaaaaga gagagagaga gaagaaaaaa aaaattaccc gcactcacgg aagctgactg
     8281 cctttttctg aattacacgg tggaaagtag tgtctggtga taagagtaaa tgcggtcttg
     8341 cttttttgga aagtctgtgc tatttataca ggagtgaaaa cggaacaggt ctgggctgtt
     8401 tgtttgttta agtattttag gtaacttgag ggtaggggtt agggtagaga gaatggagca
     8461 gaagagctgc tggagcttcc agagagatcc tcggccagag gcacccccac ccccacgccc
     8521 cctgcccgcg ctctcccgct ccctcccctc cagctgctgc acaaagcggg cgcccttccc
     8581 ggcgtgtctg ggcggtcggc tgcgtggggg cgaccagatg gaagcttgga cggatccacc
     8641 ggtcatggat agcactgaga acgtcatcaa gcccttcatg cgcttcaagg tgcacatgga
     8701 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggca agccctacga
     8761 gggcacccag accgccaagc tgcaggtgac caagggcggc cccctgccct tcgcctggga
     8821 catcctgtcc ccccagttcc agtacggctc caaggtgtac gtgaagcacc ccgccgacat
     8881 ccccgactac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     8941 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcaccttcat
     9001 ctaccacgtg aagttcatcg gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa
     9061 gactctgggc tgggagccct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     9121 cgagatccac aaggcgctga agctgaaggg cggcggccac tacctggtgg agttcaagtc
     9181 aatctacatg gccaagaagc ccgtgaagct gcccggctac tactacgtgg actccaagct
     9241 ggacatcacc tcccacaacg aggactacac cgtggtggag cagtacgagc gcgccgaggc
     9301 ccgccaccac ctgttccagt gagcggccgc gactctagat aattctaccg ggtaggggag
     9361 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     9421 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     9481 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     9541 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     9601 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     9661 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     9721 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     9781 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     9841 ggcctttcga cctgcagccc aagcttacca tgassgrgkr swm
//
