LOCUS       sequence-353436-       10264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1964
FEATURES             Location/Qualifiers
     source          1..10264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1194..1203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3202..3790
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3255..3275)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3673..3684)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3862..4095
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4173..4294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4210..4229)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4264..4283
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4334..4469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4489..4508)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4490..4508)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4518..4535)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4518..4534)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4527..4549)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4676..5131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4763..4782)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4973..4994
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5157..5261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5262..5330,5331..6122)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5480..5499)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6293..6881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6782..6801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7035..7052
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7169..7190
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7205..7210,7211..7228,7229..7235)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7243..7259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7248..7270
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7267..7283
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7267..7283
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7302..7322
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7304..7322
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7350..7576
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7577..7757
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7804..7929
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8422..8655
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8840..8884
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9115..9325
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9137..9333
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9184..9319
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9365..9967
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9365..9384)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9861..9881
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10010..10127
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 ggccaatgtg ggatcgatgt ttcaatattg gaagcgcttt gatttacagc agctgcagag
     1261 agaactcgat gccaccgcaa cggtattggc gaaccggcag gatgaaagtg agcagtccag
     1321 aaagcggctt atcgaacaga gccgggagtt caagaagaac actccagagg atttgcgcaa
     1381 gcaggtagcg ccgctgctga agagtttcca aggagagatt gatgcactga gtaaaagaag
     1441 caaggaagct gaagcagctt tcttgaatgt ctacaaaaga ttgattgacg tcccagaggt
     1501 tacgataaaa gcacttaaag agaaaatccg agaatatgaa cagacactga agaaccaagc
     1561 cgaaaccata gctcttgaga aggaacagaa gttacagaat gactttgcag aaaaggagag
     1621 aaagctgcag gagacacaga tgtccaccac ctcaaagctg gaggaagctg agcataaggt
     1681 tcagagccta caaacagccc tggaaaaaac tcgaacagaa ttatttgacc tgaaaaccaa
     1741 atacgatgaa gaaactactg caaaggccga cgagattgaa atgatcatga cggaccttga
     1801 aagggcaaac cagagggcag aggtggctca gagagaggcg gagaccttaa gggaacagct
     1861 ctcatcggcc aatcactccc tccagctggc ctcacagatc cagaaggcac cagacgtgga
     1921 gcaggccata gaggtgctga cccgctccag cctagaagtt gagttggccg ccaaggagcg
     1981 ggagatcgca cagctggtgg aggacgtgca gagactccag gccagcctca ccaagctgcg
     2041 ggagaattcg gccagccaga tctcacagct tgagcagcag ctgagcgcca aaaacagcac
     2101 actcaaacaa ctggaagaaa aactcaaagg ccaggctgac tatgaagagg tgaagaaaga
     2161 gctgaacatt ctgaagtcca tggagtttgc accgtccgag ggcgctggga cacaggatgc
     2221 ggccaagccc ctggaggtgc tgttgctgga gaagaaccgc tcgctgcagt ccgagaacgc
     2281 cgcgctgcgc atctccaaca gcgacctgag cggacgctgt gcggagctgc aagtccgtat
     2341 cactgaggct gtggccacag ccactgagca gagagagctg atcgcccgcc tggagcagga
     2401 cctgagcatc attcagtcca tccagcggcc cgatgccgag ggtgccgctg agcaccgcct
     2461 ggagaagatc ccagagccca tcaaagaggc cactgcccta ttctacggac ctgcagcacc
     2521 agccagcggt gccctcccag agggccaggt ggattcactg ctttccatca tctccagcca
     2581 gagggagcgc ttccgtgccc ggaaccagga gcttgaggcc gagaaccgcc tggcccagca
     2641 caccctccag gccctgcaga gtgagctgga cagcctgcgc gccgacaaca tcaagctctt
     2701 tgagaagatc aagttcctgc agagctaccc tggccggggc agcggcagtg atgacacgga
     2761 gctgcggtac tcgtcccagt acgaggagcg cctggacccc ttctcctcct tcagcaagcg
     2821 ggagcggcag aggaagtacc tgagcttgag tccctgggac aaggccaccc tcagcatggg
     2881 gcgtctggtt ctctccaaca agatggcgcg caccatcggc ttcttctaca cactgttcct
     2941 gcactgcctg gtcttcctgg tgctctacaa gctggcatgg agcgagagca tggagaggga
     3001 ctgtgccacc ttctgcgcca agaagttcgc tgaccacctg cacaagttcc acgagaatga
     3061 caacggggct gcggctggtg acttgtggca gtagtaatga actagtccgt aacttgaaag
     3121 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaaacagac ctgtaataga
     3181 gtccctacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     3241 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3301 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3361 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3421 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3481 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3541 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     3601 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3661 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3721 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3781 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     3841 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3901 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3961 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4021 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4081 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     4141 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     4201 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4261 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     4321 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4381 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4441 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     4501 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4561 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4621 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4681 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4741 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4801 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4861 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4921 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4981 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5041 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5101 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5161 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5221 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5281 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5341 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5401 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5461 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5521 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5581 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5641 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5701 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5761 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5821 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5881 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5941 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6001 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6061 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6121 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6181 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6241 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6421 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6901 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6961 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7021 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7081 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7141 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7201 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7261 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     7321 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     7381 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     7441 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     7501 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     7561 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7621 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7681 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7741 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     7801 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     7861 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     7921 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     7981 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8041 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8101 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8161 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8221 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8281 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8341 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8401 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8461 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8521 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8581 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8641 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     8701 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     8761 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     8821 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     8881 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8941 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9001 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     9061 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9121 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9181 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9241 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9301 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     9361 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     9421 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     9481 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     9541 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     9601 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9661 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9721 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9781 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9841 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9901 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9961 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
    10021 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    10081 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    10141 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
    10201 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
    10261 aatt
//
