LOCUS       sequence-353633-        9760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0235
FEATURES             Location/Qualifiers
     source          1..9760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2698..3286
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2751..2771)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3169..3180)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3358..3591
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3669..3790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3706..3725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3760..3779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3830..3965
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3985..4004)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3986..4004)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4014..4031)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4014..4030)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4023..4045)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4172..4627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4259..4278)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4469..4490
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4653..4757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4758..4826,4827..5618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4976..4995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5789..6377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6278..6297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6531..6548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6665..6686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6701..6706,6707..6724,6725..6731)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6739..6755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6744..6766
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6763..6779
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6763..6779
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6798..6818
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6800..6818
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6846..7072
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7073..7253
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7300..7425
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7918..8151
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8336..8380
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8611..8821
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8633..8829
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8680..8815
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8861..9463
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8861..8880)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9357..9377
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9506..9623
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcc
     1201 gagctgctcc acgtccacca tgccgggcat gatctgcaag aacccagacc tcgagtttga
     1261 ctcgctacag ccctgcttct acccggacga agatgacttc tacttcggcg gccccgactc
     1321 gacccccccg ggggaggaca tctggaagaa gtttgagctg ctgcccacgc ccccgctgtc
     1381 gcccagccgt ggcttcgcgg agcacagctc cgagcccccg agctgggtca cggagatgct
     1441 gcttgagaac gagctgtggg gcagcccggc cgaggaggac gcgttcggcc tggggggact
     1501 gggtggcctc acccccaacc cggtcatcct ccaggactgc atgtggagcg gcttctccgc
     1561 ccgcgagaag ctggagcgcg ccgtgagcga gaagctgcag cacggccgcg ggccgccaac
     1621 cgccggttcc accgcccagt ccccgggagc cggcgccgcc agccctgcgg gtcgcgggca
     1681 cggcggggct gcgggagccg gccgcgccgg ggccgccctg cccgccgagc tcgcccaccc
     1741 ggccgccgag tgcgtggatc ccgccgtggt cttccccttt cccgtgaaca agcgcgagcc
     1801 agcgcccgtg cccgcagccc cggccagtgc cccggcggcg ggccctgcgg tcgcctcggg
     1861 ggcgggtatt gccgccccag ccggggcccc gggggtcgcc cctccgcgcc caggcggccg
     1921 ccagaccagc ggcggcgacc acaaggccct cagtacctcc ggagaggaca ccctgagcga
     1981 ttcagatgat gaagatgatg aagaggaaga tgaagaggaa gaaatcgacg tggtcactgt
     2041 ggagaagcgg cgttcctcct ccaacaccaa ggctgtcacc acattcacca tcactgtgcg
     2101 tcccaagaac gcagccctgg gtcccgggag ggctcagtcc agcgagctga tcctcaaacg
     2161 atgccttccc atccaccagc agcacaacta tgccgccccc tctccctacg tggagagtga
     2221 ggatgcaccc ccacagaaga agataaagag cgaggcgtcc ccacgtccgc tcaagagtgt
     2281 catcccccca aaggctaaga gcttgagccc ccgaaactct gactcggagg acagtgagcg
     2341 tcgcagaaac cacaacatcc tggagcgcca gcgccgcaac gaccttcggt ccagctttct
     2401 cacgctcagg gaccacgtgc cggagttggt aaagaatgag aaggccgcca aggtggtcat
     2461 tttgaaaaag gccactgagt atgtccactc cctccaggcc gaggagcacc agcttttgct
     2521 ggaaaaggaa aaattgcagg caagacagca gcagttgcta aagaaaattg aacacgctcg
     2581 gacttgctag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     2641 atcttgtgga aaggacgaaa gacgcacctc ggatatgtcc gcacgcgtta agtcgacaat
     2701 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2761 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2821 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2881 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2941 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3001 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3061 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3121 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3181 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3241 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     3301 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3361 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3421 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3481 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3541 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     3601 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     3661 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3781 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     3841 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3901 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     3961 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4021 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4081 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4141 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4201 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4261 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4321 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4381 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4441 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4501 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4561 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4621 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4681 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4741 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4801 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4861 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4921 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4981 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5041 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5101 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5161 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5221 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5281 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5341 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5401 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5461 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5521 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5581 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5641 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5761 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5821 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5881 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5941 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6001 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6061 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6121 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6181 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6241 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6301 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6361 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6421 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6481 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6541 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6601 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6661 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6721 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     6781 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     6841 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     6901 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     6961 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7021 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7081 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7141 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7201 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7261 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7321 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7381 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7441 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     7501 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     7561 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     7621 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     7681 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     7741 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     7801 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     7861 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     7921 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     7981 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8041 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8101 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8161 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8221 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     8281 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     8341 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     8401 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     8461 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     8521 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     8581 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     8641 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8701 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8761 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8821 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     8881 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     8941 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9001 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9061 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9121 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9181 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9241 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9301 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9361 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9421 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
     9481 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9541 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9601 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9661 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
     9721 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
