LOCUS       sequence-353684-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, 
            R1379A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Su Y, Iacob RE, Li J, Engen JR, Springer TA
  TITLE     Dynamics of integrin alpha5beta1, fibronectin, and their complex 
            reveal sites of interaction and conformational change.
  JOURNAL   J Biol Chem. 2022 Sep;298(9):102323. doi: 10.1016/j.jbc.2022.102323.
            Epub 2022 Aug 2.
  PUBMED    35931112
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-09"; volume: "298(9):102323. doi"; pages: " 
            10.1016/j.jbc.2022.102323. Epub 2022 Aug 2"
COMMENT     Sequence Label: FnRPR9-10
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..30
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     CDS             31..51
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgcaccacc accaccatca tcaccatcac gaaaacttat acttccaggg tacaggtctt
       61 gattccccaa ctggcattga cttttctgat attactgcca actcttttac tgtgcactgg
      121 attgctcctc gagccaccat cactggctac aggatccgcc atcatcccga gcacttcagt
      181 gggagacctc gagaagatgc ggtggcgcac tctgcgaatt ccatcaccct caccaacctc
      241 actccaggca cagagtatgt ggtcagcatc gttgctctta atggcagaga ggaaagtccc
      301 ttattgattg gccaacaatc aacagtttct gatgttccga gggacctgga agttgttgct
      361 gcgaccccca ccagcctact gatcagctgg gatgctcctg ctgtcacagt gagatattac
      421 aggatcactt acggagagac aggaggaaat agccctgtcc aggagttcac tgtgcctggg
      481 agcaagtcta cagctaccat cagcggcctt aaacctggag ttgattatac catcactgtg
      541 tatgctgtca ctggccgtgg agacagcccc gcaagcagca agccaatttc cattaattac
      601 cgaacatgat ag
//
