LOCUS       sequence-353911-       10091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral transduction of human TGM3 (with a C-terminal Flag tag) 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Lee WS, Al-Ramahi I, Jeong HH, Jang Y, Lin T, Adamski CJ, Lavery LA,
            Rath S, Richman R, Bondar VV, Alcala E, Revelli JP, Orr HT, Liu Z, 
            Botas J, Zoghbi HY
  TITLE     Cross-species genetic screens identify transglutaminase 5 as a 
            regulator of polyglutamine-expanded ataxin-1.
  JOURNAL   J Clin Invest. 2022 May 2;132(9). pii: 156616. doi: 
            10.1172/JCI156616.
  PUBMED    35499073
REFERENCE   2  (bases 1 to 10091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-05-2"; volume: "132(9). pii: 156616. doi"; 
            pages: " 10.1172/JCI156616"
COMMENT     Sequence Label: W118-1_hTGM3-Flag
FEATURES             Location/Qualifiers
     source          1..10091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3143..3167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5258..5281
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(5286..5310)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5341..5929
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5394..5414)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5812..5823)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5943..6442
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5978..6001)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6375..6394
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6463..7062
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6463..6482)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6959..6979
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7200..7433
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7505..7626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7542..7561)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7596..7615
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7666..7801
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7821..7840)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7822..7840)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7850..7867)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7850..7866)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7859..7881)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8008..8463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8095..8114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8305..8326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8489..8593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8594..8662,8663..9454)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8812..8831)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9625..10091,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttca ccatggctgc
     3181 tctaggagtc cagagtatca actggcagac ggccttcaac cgacaagcgc atcacacaga
     3241 caagttctcc agccaggagc tcatcttgcg gagaggccaa aacttccagg tcttaatgat
     3301 catgaacaaa ggccttggct ctaacgaaag actggagttc attgtctcca cagggcctta
     3361 cccctcagag tcggccatga cgaaggctgt gtttccactc tccaatggca gtagtggtgg
     3421 ctggagtgcg gtgcttcagg ccagcaatgg caatactctg actatcagca tctccagtcc
     3481 tgccagcgca cccataggac ggtacacaat ggccctccag atcttctccc agggcggcat
     3541 ctcctctgtg aaacttggga cgttcatact gctttttaac ccctggctga atgtggatag
     3601 cgtctttatg ggtaaccacg ctgagagaga agagtatgtt caggaagatg ccggcatcat
     3661 ctttgtggga agcacaaacc gaattggcat gattggctgg aactttggac agtttgaaga
     3721 agacattctc agcatctgcc tctcaatctt ggataggagt ctgaatttcc gccgtgacgc
     3781 tgctactgat gtggccagca gaaatgaccc caaatacgtt ggccgggtgc tgagtgccat
     3841 gatcaatagc aatgatgaca atggtgtgct tgctgggaat tggagcggca cttacaccgg
     3901 tggccgggac ccaaggagct ggaacggcag cgtggagatc ctcaaaaatt ggaaaaaatc
     3961 tggcttcagc ccagtccgat atggccagtg ctgggtcttt gctgggaccc tcaacacagc
     4021 gctgcggtct ttggggattc cttcccgggt gatcaccaac ttcaactcag ctcatgacac
     4081 agaccgaaat ctcagtgtgg atgtgtacta cgaccccatg ggaaaccccc tggacaaggg
     4141 tagtgatagc gtatggaatt tccatgtctg gaatgaaggc tggtttgtga ggtctgacct
     4201 gggcccctcg tacggtggat ggcaggtgtt ggatgctacc ccgcaggaaa gaagccaagg
     4261 ggtgttccag tgcggccccg cttcggtcat tggtgttcga gagggtgatg tgcagctgaa
     4321 cttcgacatg ccctttatct tcgcggaggt taatgccgac cgcatcacct ggctgtacga
     4381 caacaccact ggcaaacagt ggaagaattc cgtgaacagt cacaccattg gcaggtacat
     4441 cagcaccaag gcggtgggca gcaatgctcg catggacgtc acggacaagt acaagtaccc
     4501 agaaggctct gaccaggaaa gacaagtgtt ccaaaaggct ttggggaaac ttaaacccaa
     4561 cacgccattt gccgcgacgt cttcaatggg tttggaaaca gaggaacagg agcccagcat
     4621 catcgggaag ctgaaggtcg ctggcatgct ggcagtaggc aaagaagtca acctggtcct
     4681 actgctcaaa aacctgagca gggatacgaa gacagtgaca gtgaacatga cagcctggac
     4741 catcatctac aacggcacgc ttgtacatga agtgtggaag gactctgcca caatgtccct
     4801 ggaccctgag gaagaggcag aacatcccat aaagatctcg tacgctcagt atgagaagta
     4861 cctgaagtca gacaacatga tccggatcac agcggtgtgc aaggtcccag atgagtctga
     4921 ggtggtggtg gagcgggaca tcatcctgga caaccccacc ttgaccctgg aggtgctgaa
     4981 cgaggctcgt gtgcggaagc ctgtgaacgt gcagatgctc ttctccaatc cactggatga
     5041 gccggtgagg gactgcgtgc tgatggtgga gggaagcggc ctgctgttgg gtaacctgaa
     5101 gatcgacgtg ccgaccctag ggcccaagga ggggtcccgg gtccgttttg atatcctgcc
     5161 ctcccggagt ggcaccaagc aactgctcgc cgacttctcc tgcaacaagt tccctgcaat
     5221 caaggccatg ttgtccatcg atgtggccga aggcggcgac tacaaagacg atgacgacaa
     5281 gtgagaccca gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgac
     5341 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5401 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5461 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5521 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5581 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5641 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5701 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     5761 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5821 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5881 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcta
     5941 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     6001 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     6061 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     6121 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     6181 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     6241 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     6301 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     6361 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     6421 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
     6481 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6541 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6601 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6661 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6721 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6781 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6841 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6901 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6961 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     7021 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     7081 cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg tacctttaag
     7141 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7201 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     7261 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7321 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7381 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7441 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7501 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7561 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7621 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     7681 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7741 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7801 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     7861 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7921 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     7981 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     8041 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8101 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8161 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8221 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8281 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     8341 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8401 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     8461 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8521 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8581 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8641 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8701 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8761 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8821 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8881 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8941 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9001 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9061 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9121 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9181 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9241 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9301 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9361 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9421 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9481 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9541 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9601 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9661 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9721 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9781 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9841 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9901 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9961 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10021 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10081 cggaacagga g
//
