LOCUS       sequence-353917-        4671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A9_STOP
FEATURES             Location/Qualifiers
     source          1..4671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2228..2327)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2344..2363)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2345..2363)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2368..2384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2368..2384)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2486..2505)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2497..3306
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2564..2583)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3453..4041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3942..3961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4195..4212
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4371..4398)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4490..4576)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4625..4647
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4639..4656
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4640..4656
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag cggaggcgac
      121 accagggcag caatcgcacg gcccagaatg gccgccgccc acggccctgt ggccccaagc
      181 tcccccgagc aggtgacact gctgccagtg cagagatcct tctttctgcc acctttctct
      241 ggagcaaccc catccacatc tctggcagag tccgtgctga aggtctggca cggcgcctac
      301 aactctggcc tgctgcctca gctgatggcc cagcacagcc tggcaatggc acagaacgga
      361 gcagtgccat ccgaggcaac caagcgggat cagaatctga agagaggcaa ctggggcaat
      421 cagatcgagt ttgtgctgac atccgtggga tacgcagtgg gactgggaaa cgtgtggagg
      481 ttcccatacc tgtgctatcg caatggcggc ggcgccttta tgttccccta cttcatcatg
      541 ctgatcttct gtggcatccc tctgttcttt atggagctga gctttggcca gttcgcatcc
      601 cagggatgcc tgggcgtgtg gaggatctct cctatgttta agggcgtggg ctacggcatg
      661 atggtggtga gcacctatat cggcatctac tataacgtgg tcatctgtat cgccttctac
      721 tatttctttt ctagcatgac acacgtgctg ccctgggcct actgcaacaa tccttggaat
      781 acccacgact gtgccggcgt gctggatgcc tctaacctga caaatggaag cagaccagcc
      841 gccctgccat ccaacctgtc tcacctgctg aatcactccc tgcagcggac cagcccatcc
      901 gaggagtact ggagactgta tgtgctgaag ctgtctgacg atatcggcaa ttttggagag
      961 gtgcggctgc cactgctggg atgcctgggc gtgagctggc tggtggtgtt cctgtgcctg
     1021 atcagaggcg tgaagtcctc tggcaaggtg gtgtacttta ccgccacatt cccttatgtg
     1081 gtgctgacca tcctgtttgt gaggggagtg acactggagg gagccttcga cggcatcatg
     1141 tactatctga ccccacagtg ggacaagatc ctggaggcaa aagtgtgggg cgatgcagca
     1201 tcccagatct tttactctct gggatgcgca tggggaggac tgatcacaat ggccagctac
     1261 aacaagttcc acaacaattg ctatagggat agcgtgatca tctccatcac caattgtgcc
     1321 acaagcgtgt acgccggctt tgtgatcttc tccatcctgg gctttatggc aaaccacctg
     1381 ggagtggacg tgtccagggt ggcagatcac ggaccaggac tggccttcgt ggcatatcca
     1441 gaggccctga ccctgctgcc tatctctcca ctgtggagcc tgctgttctt tttcatgctg
     1501 atcctgctgg gcctgggcac acagttctgt ctgctggaga ccctggtgac agccatcgtg
     1561 gacgaagtgg gcaatgagtg gatcctgcag aagaagacct acgtgacact gggagtggca
     1621 gtggcaggct ttctgctggg aatcccactg acctctcagg caggcatcta ctggctgctg
     1681 ctgatggaca actatgccgc ctctttcagc ctggtggtca tctcctgcat catgtgcgtg
     1741 gccatcatgt acatctatgg ccacaggaat tattttcagg atatccagat gatgctgggc
     1801 ttcccacccc ctctgttttt ccagatctgc tggcgctttg tgagccccgc catcatcttt
     1861 ttcatcctgg tgttcaccgt gatccagtac cagcctatca catataacca ctaccagtat
     1921 ccaggctggg ccgtggccat cggctttctg atggccctga gctccgtgct gtgcatccct
     1981 ctgtacgcca tgttcaggct gtgccgcacc gacggcgata cactgctgca gaggctgaag
     2041 aatgccacca agcctagcag ggactgggga ccagccctgc tggagcaccg gaccggcaga
     2101 tatgcaccaa caatcgcacc atccccagag gacggattcg aggtgcagcc actgcaccct
     2161 gataaggccc agatccctat cgtgggctct aacggctcta gcaggctgca ggatagccgc
     2221 atctaagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2281 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2341 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2401 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     2461 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     2521 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2581 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     2641 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2701 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2761 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     2821 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2881 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2941 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     3001 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     3061 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     3121 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     3181 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     3241 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3301 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3361 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     3421 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3481 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3541 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3601 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3661 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3721 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3781 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3841 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3901 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3961 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4021 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4081 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4141 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4201 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4261 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4321 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     4381 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     4441 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     4501 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     4561 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     4621 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
