LOCUS       sequence-354049-       11654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac compatible plasmid expressing dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11654)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 11654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: NAX_CAGGS_CAS9_m4_Blasto
FEATURES             Location/Qualifiers
     source          1..11654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          128..1144
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1068..1090
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1152..1171
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1241..1250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1247..1273,1274..1276,1277..3760,3761..3766,3767..3832,
                     3833..3835,3836..5350)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5363..5383
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5408..5427)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5452..5500
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5732..5943
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      5953..5962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5959..6357
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6361..6482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6398..6417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6452..6471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7245..7279
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7770..7786)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7770..7786)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7783..7805)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7794..7810
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7818..7824,7825..7842,7843..7848))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7863..7884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8001..8018)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8172..8760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8252..8271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8931..9722,9723..9791))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9554..9573
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9792..9896)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9922..10377)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10059..10080)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10271..10290
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10504..10526
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10518..10535
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10519..10535
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   10665..10727
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        11148..11527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(11529..11654,1..127)
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
ORIGIN
        1 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
       61 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      121 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
      181 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
      241 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
      301 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
      361 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
      421 tgagcgctgc gggcgcggcg cggggctttt tgcgctccgc agtgtgcgcg aggggagcgc
      481 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
      541 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
      601 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
      661 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
      721 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
      781 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
      841 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
      901 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
      961 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     1021 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     1081 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     1141 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaatctag
     1201 ataccctcgt aaaggtctag agctagcgga tcgaaccctt gccaccatgg acaagaagta
     1261 ctccattggg ctcgctatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta
     1321 caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa
     1381 gaacctcatt ggcgccctcc tgttcgactc cggggagacg gccgaagcca cgcggctcaa
     1441 aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat
     1501 ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt
     1561 tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga
     1621 ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag
     1681 tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg
     1741 gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt
     1801 tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg
     1861 agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct
     1921 catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc
     1981 actcgggctg acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca
     2041 actgagcaaa gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca
     2101 gtacgcagac ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat
     2161 tctgcgagtg aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta
     2221 tgatgagcac caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga
     2281 gaagtacaag gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg
     2341 cggagcaagc caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg
     2401 caccgaggag ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt
     2461 cgacaatgga agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg
     2521 gcaagaggat ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac
     2581 atttcggata ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat
     2641 gactcgcaaa tcagaagaga ccatcactcc ctggaacttc gaggaagtcg tggataaggg
     2701 ggcctctgcc cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga
     2761 aaaggtgctt cctaaacact ctctgctgta cgagtacttc acagtttata acgagctcac
     2821 caaggtcaaa tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa
     2881 gaaagctatc gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa
     2941 agaagactat ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga
     3001 tcgcttcaac gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga
     3061 cttcctggac aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt
     3121 gtttgaagat agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga
     3181 caaagtcatg aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa
     3241 actgatcaat gggatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc
     3301 cgatggattt gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa
     3361 ggaggacatc cagaaagcac aagtttctgg ccagggggac agtcttcacg agcacatcgc
     3421 taatcttgca ggtagcccag ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga
     3481 tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg
     3541 agagaaccaa actacccaga agggacagaa gaacagtagg gaaaggatga agaggattga
     3601 agagggtata aaagaactgg ggtcccaaat ccttaaggaa cacccagttg aaaacaccca
     3661 gcttcagaat gagaagctct acctgtacta cctgcagaac ggcagggaca tgtacgtgga
     3721 tcaggaactg gacatcaatc ggctctccga ctacgacgtg gctgctatcg tgccccagtc
     3781 ttttctcaaa gatgattcta ttgataataa agtgttgaca agatccgata aagctagagg
     3841 gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt attggcggca
     3901 gctgctgaac gccaaactga tcacacaacg gaagttcgat aatctgacta aggctgaacg
     3961 aggtggcctg tctgagttgg ataaagccgg cttcatcaaa aggcagcttg ttgagacacg
     4021 ccagatcacc aagcacgtgg cccaaattct cgattcacgc atgaacacca agtacgatga
     4081 aaatgacaaa ctgattcgag aggtgaaagt tattactctg aagtctaagc tggtctcaga
     4141 tttcagaaag gactttcagt tttataaggt gagagagatc aacaattacc accatgcgca
     4201 tgatgcctac ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc ccaagcttga
     4261 atctgaattt gtttacggag actataaagt gtacgatgtt aggaaaatga tcgcaaagtc
     4321 tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt tacagcaata ttatgaattt
     4381 tttcaagacc gagattacac tggccaatgg agagattcgg aagcgaccac ttatcgaaac
     4441 aaacggagaa acaggagaaa tcgtgtggga caagggtagg gatttcgcga cagtccggaa
     4501 ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc gaagtacaga ccggaggctt
     4561 ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga
     4621 ttgggacccc aagaaatacg gcggattcga ttctcctaca gtcgcttaca gtgtactggt
     4681 tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg
     4741 catcacaatc atggagcgat caagcttcga aaaaaacccc atcgactttc tcgaggcgaa
     4801 aggatataaa gaggtcaaaa aagacctcat cattaagctt cccaagtact ctctctttga
     4861 gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga
     4921 gctggcactg ccctctaaat acgttaattt cttgtatctg gccagccact atgaaaagct
     4981 caaagggtct cccgaagata atgagcagaa gcagctgttc gtggaacaac acaaacacta
     5041 ccttgatgag atcatcgagc aaataagcga attctccaaa agagtgatcc tcgccgacgc
     5101 taacctcgat aaggtgcttt ctgcttacaa taagcacagg gataagccca tcagggagca
     5161 ggcagaaaac attatccact tgtttactct gaccaacttg ggcgcgcctg cagccttcaa
     5221 gtacttcgac accaccatag acagaaagcg gtacacctct acaaaggagg tcctggacgc
     5281 cacactgatt catcagtcaa ttacggggct ctatgaaaca agaatcgacc tctctcagct
     5341 cggtggagac agcagggctg accccaagaa gaagaggaag gtgtgagttt aaacggggga
     5401 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     5461 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     5521 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5581 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5641 gtcggggcgg caggccctgc catagcagat ctgcgaaatc ggatccgcgg ccgcactgat
     5701 tctagctgca gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     5761 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     5821 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     5881 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     5941 caggatcctc gagccaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
     6001 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     6061 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     6121 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     6181 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     6241 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     6301 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagtt
     6361 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6421 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6481 tatcatgtct ggaattgact caaatgatgt caattagtct atcagaagct catctggtct
     6541 cccttccggg ggacaagaca tccctgttta atatttaaac agcagtgttc ccaaactggg
     6601 ttcttatatc ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc acaggaacga
     6661 agtccctaaa gaaacagtgg cagccaggtt tagccccgga attgactgga ttcctttttt
     6721 agggcccatt ggtatggctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc
     6781 agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc
     6841 cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg
     6901 ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg
     6961 ggctgtccct gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga
     7021 acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa
     7081 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat
     7141 tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga
     7201 cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac
     7261 gcagactatc tttctagggt taatctagct gcatcaggat catatcgtcg ggtctttttt
     7321 ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc
     7381 cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat
     7441 tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc
     7501 ctttaacggt gaactgttcg ttcaggccac ctgggatacc agttcgtcgc ggcttttccg
     7561 gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag
     7621 ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt
     7681 cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt
     7741 acccggcggg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7801 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7861 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7921 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7981 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8041 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8101 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8161 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8221 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8281 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8341 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8401 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8461 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8521 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8581 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8641 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8701 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8761 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8821 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8881 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8941 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9001 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9061 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9121 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9181 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9241 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9301 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9361 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9421 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9481 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9541 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9601 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9661 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9721 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9781 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9841 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9901 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     9961 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
    10021 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
    10081 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
    10141 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
    10201 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
    10261 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
    10321 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
    10381 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    10441 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    10501 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
    10561 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
    10621 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
    10681 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
    10741 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
    10801 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
    10861 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
    10921 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
    10981 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
    11041 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
    11101 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccgac attgattatt
    11161 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
    11221 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    11281 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
    11341 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
    11401 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
    11461 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
    11521 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
    11581 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
    11641 gggggggggg gggc
//
