LOCUS       sequence-354117-        9424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 sgRNA cloning plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: pSB700 lenti gRNA Hygro
FEATURES             Location/Qualifiers
     source          1..9424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          176..1192
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1116..1138
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1200..1219
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1220..1229
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1241..2260
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             1241..2260
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    2284..2872
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2337..2357)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2755..2766)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3079..3712
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3841..3857)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3841..3857)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3854..3876)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3865..3881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3889..3895,3896..3913,3914..3919))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3934..3955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4072..4089)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4243..4831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4323..4342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5002..5793,5794..5862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5625..5644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5863..5967)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6015..6149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6052..6071)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6106..6125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6317..6950
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6997..7122
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7619..7852
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8037..8081
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8344..8459
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        8562..8810
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8562..8582
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8733..8752
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8832..8907
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8981..9284
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
       61 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
      121 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
      181 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
      241 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      301 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
      361 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
      421 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
      481 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
      541 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
      601 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
      661 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
      721 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
      781 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
      841 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
      901 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
      961 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     1021 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     1081 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     1141 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     1201 caacgtgctg gttattgtgg ccaccatgga tagatccgga aagcctgaac tcaccgcgac
     1261 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtt tccgacctga tgcagctctc
     1321 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     1381 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     1441 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     1501 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     1561 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     1621 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     1681 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     1741 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     1801 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     1861 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     1921 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     1981 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     2041 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     2101 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     2161 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     2221 aaaccgacgc cccagcactc gtccgagggc aaaggaatag tgtacaagta aagcgtctgg
     2281 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2341 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2401 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2461 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2521 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2581 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2641 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     2701 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     2761 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     2821 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     2881 aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     2941 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     3001 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3061 taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     3121 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     3181 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     3241 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     3301 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     3361 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     3421 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     3481 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     3541 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3601 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     3661 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     3721 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     3781 aggccttgac attgctagcg ttttaccgtc gacctctagc tagagcttgg cgtaatcatg
     3841 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     3901 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     3961 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4021 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4081 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4141 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4201 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4261 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4321 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4381 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4441 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4501 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4561 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4621 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4681 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4741 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4801 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4861 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4921 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4981 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5041 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5101 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5161 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5221 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5281 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5341 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5401 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5461 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5521 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5581 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5641 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5701 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5761 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5821 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5881 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5941 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     6001 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6061 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6121 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     6181 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     6241 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     6301 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     6361 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     6421 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     6481 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     6541 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     6601 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     6661 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     6721 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     6781 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6841 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6901 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     6961 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7021 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7081 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7141 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7201 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7261 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7321 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7381 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7441 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     7501 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     7561 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     7621 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     7681 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     7741 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     7801 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     7861 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     7921 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     7981 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8041 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     8101 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     8161 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     8221 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     8281 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     8341 gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     8401 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     8461 cgggtttatt acagggacag cagagatcca gtttatcatt agtgaacgga tctcgacggt
     8521 atcgatcacg agactagcct cgagcggccg cccccttcac cgagggccta tttcccatga
     8581 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga
     8641 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     8701 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     8761 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc ggagacgatt
     8821 aatgcgtctc ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     8881 cttgaaaaag tggcaccgag tcggtgcttt ttttgaattc tcgacctcga gacaaatggc
     8941 agtattcgtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     9001 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     9061 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     9121 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     9181 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     9241 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     9301 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     9361 ttttttaatt attttgtgca gcgatggggg cggggggggg gggcgcgcgc caggcggggc
     9421 gggg
//
