LOCUS       sequence-354128-        9118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: pSB700 lenti gRNA KanR neo_TtoC and hairpin
            ex...
FEATURES             Location/Qualifiers
     source          1..9118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          122..1138
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1062..1084
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1146..1165
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1172..1966
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1172..1966
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1226..1245)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1836..1855
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    1990..2578
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2043..2063)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2461..2472)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2785..3418
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3547..3563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3547..3563)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3560..3582)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3571..3587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3595..3601,3602..3619,3620..3625))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3640..3661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3778..3795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3949..4537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4029..4048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4708..5499,5500..5568))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5331..5350
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5569..5673)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5721..5855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5758..5777)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5812..5831
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6023..6656
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6703..6828
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7325..7558
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7743..7787
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8050..8165
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        8268..8516
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8268..8288
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8439..8458
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        8697..9000
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
       61 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      121 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      181 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      241 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      301 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      361 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      421 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
      481 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
      541 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
      601 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
      661 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
      721 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
      781 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
      841 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
      901 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
      961 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1021 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1081 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1141 cctgggcaac gtgctggtta ttgtggccac catgattgaa caagatggat tgcacgcagg
     1201 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     1261 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     1321 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     1381 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     1441 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     1501 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     1561 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     1621 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     1681 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     1741 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     1801 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     1861 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     1921 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgatgta caagtaaagc
     1981 gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     2041 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     2101 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     2161 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     2221 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     2281 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     2341 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     2401 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     2461 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     2521 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     2581 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     2641 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     2701 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     2761 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
     2821 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     2881 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     2941 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     3001 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     3061 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     3121 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     3181 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     3241 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     3301 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     3361 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     3421 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     3481 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta
     3541 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     3601 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     3661 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     3721 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     3781 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3841 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3901 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3961 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4021 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4081 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4141 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4201 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4261 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4321 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4381 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4441 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4501 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4561 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4621 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4681 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4741 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4801 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4861 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4921 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4981 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5041 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5101 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5161 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5221 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5281 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5341 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5401 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5461 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5521 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5581 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5641 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5701 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
     5761 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5821 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     5881 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     5941 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     6001 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
     6061 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     6121 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     6181 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     6241 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     6301 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     6361 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     6421 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     6481 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6541 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6601 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     6661 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     6721 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     6781 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     6841 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     6901 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     6961 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     7021 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     7081 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     7141 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
     7201 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     7261 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     7321 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     7381 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     7441 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     7501 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     7561 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     7621 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     7681 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     7741 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     7801 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     7861 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     7921 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     7981 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     8041 ggtatcgcct ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     8101 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     8161 aattttcggg tttattacag ggacagcaga gatccagttt atcattagtg aacggatctc
     8221 gacggtatcg atcacgagac tagcctcgag cggccgcccc cttcaccgag ggcctatttc
     8281 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
     8341 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
     8401 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
     8461 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccggag
     8521 acgattaatg cgtctcggtt taagagctat gctggaaaca gcatagcaag tttaaataag
     8581 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt gaattctcga
     8641 cctcgagaca aatggcagta ttcgtcatta gttcatagcc catatatgga gttccgcgtt
     8701 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     8761 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     8821 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     8881 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     8941 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     9001 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     9061 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggg
//
