LOCUS       sequence-35450-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Fleishman SJ, Whitehead TA, Strauch EM, Corn JE, Qin S, Zhou HX, 
            Mitchell JC, Demerdash ON, Takeda-Shitaka M, Terashi G, Moal IH, Li 
            X, Bates PA, Zacharias M, Park H, Ko JS, Lee H, Seok C, Bourquard T,
            Bernauer J, Poupon A, Aze J, Soner S, Ovali SK, Ozbek P, Tal NB, 
            Haliloglu T, Hwang H, Vreven T, Pierce BG, Weng Z, Perez-Cano L, 
            Pons C, Fernandez-Recio J, Jiang F, Yang F, Gong X, Cao L, Xu X, Liu
            B, Wang P, Li C, Wang C, Robert CH, Guharoy M, Liu S, Huang Y, Li L,
            Guo D, Chen Y, Xiao Y, London N, Itzhaki Z, Schueler-Furman O, Inbar
            Y, Patapov V, Cohen M, Schreiber G, Tsuchiya Y, Kanamori E, Standley
            DM, Nakamura H, Kinoshita K, Driggers CM, Hall RG, Morgan JL, Hsu 
            VL, Zhan J, Yang Y, Zhou Y, Kastritis PL, Bonvin AM, Zhang W, 
            Camacho CJ, Kilambi KP, Sircar A, Gray JJ, Ohue M, Uchikoga N, 
            Matsuzaki Y, Ishida T, Akiyama Y, Khashan R, Bush S, Fouches D, 
            Tropsha A, Esquivel-Rodriguez J, Kihara D, Stranges PB, Jacak R, 
            Kuhlman B, Huang SY, Zou X, Wodak SJ, Janin J, Baker D
  TITLE     Community-Wide Assessment of Protein-Interface Modeling Suggests 
            Improvements to Design Methodology.
  JOURNAL   J Mol Biol. 2011 Sep 29.
  PUBMED    22001016
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2011 Sep 29."
COMMENT     Sequence Label: pETCON-HB58/design_26
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             296..307
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             308..334
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             410..427
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             836..865
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tacattttca attaagatgc agttacttcg ctgtttttca atattttctg ttattgcttc
       61 agttttagca caggaactga caactatatg cgagcaaatc ccctcaccaa ctttagaatc
      121 gacgccgtac tctttgtcaa cgactactat tttggccaac gggaaggcaa tgcaaggagt
      181 ttttgaatat tacaaatcag taacgtttgt cagtaattgc ggttctcacc cctcaacaac
      241 tagcaaaggc agccccataa acacacagta tgtttttaag gacaatagct cgacgattga
      301 aggtagatac ccatacgacg ttccagacta cgctctgcag gctagtggtg gaggaggctc
      361 tggtggaggc ggtagcggag gcggagggtc ggctagccat atgaagattc accaccatca
      421 tcaccatcct ccagaagcct actcattaga caccgccatt tttgtattag aaaccagaga
      481 ttatagatta tcttgggttt ttattgtatc atggataggt gacgttaaaa tgaatggtaa
      541 aactgccaga ttcgaaacag aatatggtcc agtcaaattg tatgtttaca agggtcaaga
      601 agctaaaaag atatggaaaa agttgaacgg tagagcaggt tttgtatcta ttagatcagt
      661 cttggactta cctaatatgg gtaaattctc caccgttagt aacggtaaaa agatcgtagc
      721 ttggtggaga aagaattggt tgtttattgt tgaaggtaag aatggtgttg aagaatttgt
      781 taagcatgtc tacagagttt atgaagaaat gaagcaactc gaggggggcg gatccgaaca
      841 aaagcttatt tctgaagagg acttgtaata gag
//
