LOCUS       sequence-354513-        9412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: pSB700 lenti gRNA Hygro_zhang2.0
FEATURES             Location/Qualifiers
     source          1..9412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          127..1143
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1067..1089
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1151..1170
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1171..1180
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1192..2211
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             1192..2211
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    2235..2823
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2288..2308)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2706..2717)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3030..3663
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3792..3808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3792..3808)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3805..3827)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3816..3832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3840..3846,3847..3864,3865..3870))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3885..3906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4023..4040)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4194..4782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4274..4293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4953..5744,5745..5813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5576..5595
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5814..5918)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5966..6100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6003..6022)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6057..6076
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6268..6901
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6948..7073
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7570..7803
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7988..8032
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8295..8410
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        8513..8761
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8513..8533
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8684..8703
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8800..8818
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8870..8888
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     enhancer        8992..9295
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggsgggggcs rggcgrggcg grgrggkgcg gcggcagcca atcagagcgg cgcgctccga
       61 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
      121 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
      181 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
      241 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
      301 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
      361 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
      421 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
      481 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
      541 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
      601 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
      661 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
      721 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc
      781 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
      841 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
      901 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
      961 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     1021 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     1081 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     1141 cagctcctgg gcaacgtgct ggttattgtg gccaccatgg atagatccgg aaagcctgaa
     1201 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ttccgacctg
     1261 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     1321 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     1381 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     1441 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     1501 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     1561 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     1621 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     1681 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     1741 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     1801 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     1861 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     1921 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     1981 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     2041 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     2101 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     2161 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gtgtacaagt
     2221 aaagcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2281 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2341 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2401 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2461 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2521 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2581 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     2641 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2701 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     2761 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     2821 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     2881 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     2941 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     3001 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
     3061 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     3121 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     3181 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     3241 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     3301 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     3361 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     3421 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     3481 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3541 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3601 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3661 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3721 cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg
     3781 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3841 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3901 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     3961 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4021 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4081 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4141 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4201 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4261 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4321 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4381 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4441 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4501 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4561 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4621 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4681 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4741 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4801 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4861 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4921 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4981 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5041 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5101 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5161 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5221 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5281 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5341 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5401 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5461 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5521 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5581 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5641 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5701 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5761 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5821 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5881 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5941 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
     6001 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6061 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     6121 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     6181 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     6241 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     6301 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     6361 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     6421 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     6481 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     6541 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     6601 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     6661 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     6721 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6781 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6841 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6901 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     6961 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     7021 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     7081 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     7141 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     7201 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     7261 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     7321 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     7381 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     7441 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     7501 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     7561 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     7621 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     7681 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     7741 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     7801 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     7861 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     7921 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     7981 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     8041 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     8101 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     8161 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     8221 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     8281 tcgacggtat cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     8341 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     8401 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttatcat tagtgaacgg
     8461 atctcgacgg tatcgatcac gagactagcc tcgagcggcc gcccccttca ccgagggcct
     8521 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattgg
     8581 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
     8641 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
     8701 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
     8761 cggagacgat taatgcgtct cggttttaga gctaggccaa catgaggatc acccatgtct
     8821 gcagggccta gcaagttaaa ataaggctag tccgttatca acttggccaa catgaggatc
     8881 acccatgtct gcagggccaa gtggcaccga gtcggtgctt tttttgaatt ctcgacctcg
     8941 agacaaatgg cagtattcgt cattagttca tagcccatat atggagttcc gcgttacata
     9001 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     9061 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     9121 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     9181 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     9241 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga
     9301 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
     9361 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg gg
//
