LOCUS       sequence-354514-        8980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible Y2H vector - BD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller 
            WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y
  TITLE     Arabidopsis homolog of the yeast TREX-2 mRNA export complex: 
            components and anchoring nucleoporin.
  JOURNAL   Plant J. 2010 Jan;61(2):259-70. doi: 
            10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16.
  PUBMED    19843313
REFERENCE   2  (bases 1 to 8980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-313X.2009.04048.x"; journalName: "Plant J"; date: 
            "2010-01"; volume: "61"; issue: "2"; pages: "259-70"
COMMENT     Sequence Label: pGBKT7-GW
FEATURES             Location/Qualifiers
     source          1..8980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     125..144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     378..395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    512..533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(548..553,554..571,572..578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    586..602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     591..613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     610..626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     610..626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(650..837)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     758..782
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(864..911)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    935..1059
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1100..1405)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1202..1221)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1747..2406)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2321..2340
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2460..2466,2467..2484,2485..2490))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2515..2639)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2646..2675)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2694..2713)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2695..2713)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2724..3164)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(3192..3896)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     rep_origin      3967..4422
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4054..4073)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4264..4285
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4525..5199)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(5200..5481)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(5502..5521)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5621..5643
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5681..5699)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      5740..7082
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    6072..6119
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7109..7213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7214..8008
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7214..8008
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7268..7287)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7878..7897
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8196..8215)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8240..8287
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      join(8616..8980,1..224)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
       61 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      301 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      361 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      421 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
      481 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      541 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      601 aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc cggtagaggt
      661 gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
      721 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      781 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgcccg
      841 gaattagctt ggctgcaagc gcgcaaaaaa cccctcaaga cccgtttaga ggccccaagg
      901 ggttatgcta gttatgcggc cgctgcaggt cgagaccact ttgtacaaga aagctgaacg
      961 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
     1021 tacataatac tgtaaaacac aacatatcca gtcactatgg tcgacctgca gactggctgt
     1081 gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt
     1141 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg
     1201 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
     1261 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
     1321 cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
     1381 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg
     1441 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
     1501 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
     1561 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
     1621 gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat
     1681 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
     1741 agatctttac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1801 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1861 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1921 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1981 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     2041 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     2101 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     2161 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
     2221 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     2281 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     2341 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2401 ctccatttta gcttccttag ctcctgaaaa tctcgacgga tcctaactca aaatccacac
     2461 attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgcgg ccgccatagt
     2521 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     2581 aatatattga tatttatatc attttacgtt tctcgttcag cttttttgta caaacttgtc
     2641 atatgcaggt cctcctctga gatcagcttc tgctcctcca tgatggcggc tcgccctata
     2701 gtgagtcgta ttacaaattc cggcgataca gtcaactgtc tttgaccttt gttactactc
     2761 tcttccgatg atgatgtcgc acttattcta tgctgtctca atgttagagg catatcagtc
     2821 tccactgaag ccaatctatc tgtgacggca tctttattca cattatcttg tacaaataat
     2881 cctgttaaca atgcttttat atcctgtaaa gaatccattt tcaaaatcat gtcaaggtct
     2941 tctcgaggaa aaatcagtag aaatagctgt tccagtcttt ctagccttga ttccacttct
     3001 gtcagatgtg ccctagtcag cggagacctt ttggttttgg gagagtagcg acactcccag
     3061 ttgttcttca gacacttggc gcacttcggt ttttctttgg agcacttgag ctttttaagt
     3121 cggcaaatat cgcatgcttg ttcgatagaa gacagtagct tcatctttca ggaggcttgc
     3181 ttcaagcttg gagttgattg tatgcttggt atagcttgaa atattgtgca gaaaaagaaa
     3241 caaggaagaa agggaacgag aacaatgacg aggaaacaaa agattaataa ttgcaggtct
     3301 atttatactt gatagcaaga cagcaaactt ttttttattt caaattcaag taactggaag
     3361 gaaggccgta taccgttgct cattagagag tagtgtgcgt gaatgaagga aggaaaaagt
     3421 ttcgtgtgct tcgagatacc cctcatcagc tctggaacaa cgacatctgt tggtgctgtc
     3481 tttgtcgtta attttttcct ttagtgtctt ccatcatttt tttgtcattg cggatatggt
     3541 gagacaacaa cgggggagag agaaaagaaa aaaaaagaaa agaagttgca tgcgcctatt
     3601 attacttcaa tagatggcaa atggaaaaag ggtagtgaaa cttcgatatg atgatggcta
     3661 tcaagtctag ggctacagta ttagttcgtt atgtaccacc atcaatgagg cagtgtaatt
     3721 ggtgtagtct tgtttagccc attatgtctt gtctggtatc tgttctattg tatatctccc
     3781 ctccgccacc tacatgttag ggagaccaac gaaggtatta taggaatccc gatgtatggg
     3841 tttggttgcc agaaaagagg aagtccatat tgtacacccg gaaacaacaa aaggatctgg
     3901 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3961 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4021 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4081 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4141 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4201 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4261 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4321 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4381 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     4441 ccttacgcat ctgtgcggta tttcacaccg catagatcgg caagtgcaca aacaatactt
     4501 aaataaatac tactcagtaa taacctattt cttagcattt ttgacgaaat ttgctatttt
     4561 gttagagtct tttacaccat ttgtctccac acctccgctt acatcaacac caataacgcc
     4621 atttaatcta agcgcatcac caacattttc tggcgtcagt ccaccagcta acataaaatg
     4681 taagctttcg gggctctctt gccttccaac ccagtcagaa atcgagttcc aatccaaaag
     4741 ttcacctgtc ccacctgctt ctgaatcaaa caagggaata aacgaatgag gtttctgtga
     4801 agctgcactg agtagtatgt tgcagtcttt tggaaatacg agtcttttaa taactggcaa
     4861 accgaggaac tcttggtatt cttgccacga ctcatctcca tgcagttgga cgatatcaat
     4921 gccgtaatca ttgaccagag ccaaaacatc ctccttaggt tgattacgaa acacgccaac
     4981 caagtatttc ggagtgcctg aactattttt atatgctttt acaagacttg aaattttcct
     5041 tgcaataacc gggtcaattg ttctctttct attgggcaca catataatac ccagcaagtc
     5101 agcatcggaa tctagagcac attctgcggc ctctgtgctc tgcaagccgc aaactttcac
     5161 caatggacca gaactacctg tgaaattaat aacagacata ctccaagctg cctttgtgtg
     5221 cttaatcacg tatactcacg tgctcaatag tcaccaatgc cctccctctt ggccctctcc
     5281 ttttcttttt tcgaccgaat taattcttaa tcggcaaaaa aagaaaagct ccggatcaag
     5341 attgtacgta aggtgacaag ctatttttca ataaagaata tcttccacta ctgccatctg
     5401 gcgtcataac tgcaaagtac acatatatta cgatgctgtt ctattaaatg cttcctatat
     5461 tatatatata gtaatgtcgt tgatctatgg tgcactctca gtacaatctg ctctgatgcc
     5521 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5581 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5641 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     5701 ttataggtta atgtcatgat aataatggtt tcttagtatg atccaatatc aaaggaaatg
     5761 atagcattga aggatgagac taatccaatt gaggagtggc agcatataga acagctaaag
     5821 ggtagtgctg aaggaagcat acgatacccc gcatggaatg ggataatatc acaggaggta
     5881 ctagactacc tttcatccta cataaataga cgcatataag tacgcattta agcataaaca
     5941 cgcactatgc cgttcttctc atgtatatat atatacaggc aacacgcaga tataggtgcg
     6001 acgtgaacag tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc
     6061 ggaaacgctt tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca
     6121 gagcgctttt gaaaaccaaa agcgctctga agacgcactt tcaaaaaacc aaaaacgcac
     6181 cggactgtaa cgagctacta aaatattgcg aataccgctt ccacaaacat tgctcaaaag
     6241 tatctctttg ctatatatct ctgtgctata tccctatata acctacccat ccacctttcg
     6301 ctccttgaac ttgcatctaa actcgacctc tacatttttt atgtttatct ctagtattac
     6361 tctttagaca aaaaaattgt agtaagaact attcatagag tgaatcgaaa acaatacgaa
     6421 aatgtaaaca tttcctatac gtagtatata gagacaaaat agaagaaacc gttcataatt
     6481 ttctgaccaa tgaagaatca tcaacgctat cactttctgt tcacaaagta tgcgcaatcc
     6541 acatcggtat agaatataat cggggatgcc tttatcttga aaaaatgcac ccgcagcttc
     6601 gctagtaatc agtaaacgcg ggaagtggag tcaggctttt tttatggaag agaaaataga
     6661 caccaaagta gccttcttct aaccttaacg gacctacagt gcaaaaagtt atcaagagac
     6721 tgcattatag agcgcacaaa ggagaaaaaa agtaatctaa gatgctttgt tagaaaaata
     6781 gcgctctcgg gatgcatttt tgtagaacaa aaaagaagta tagattcttt gttggtaaaa
     6841 tagcgctctc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa
     6901 aattagcgct ctcgcgttgc atttttgttt tacaaaaatg aagcacagat tcttcgttgg
     6961 taaaatagcg ctttcgcgtt gcatttctgt tctgtaaaaa tgcagctcag attctttgtt
     7021 tgaaaaatta gcgctctcgc gttgcatttt tgttctacaa aatgaagcac agatgcttcg
     7081 ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7141 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7201 aaaaaggaag agtatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     7261 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     7321 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     7381 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     7441 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     7501 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     7561 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     7621 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     7681 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     7741 gagcatgccc gacggcgagg atctcgtcgt gactcatggc gatgcctgct tgccgaatat
     7801 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     7861 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     7921 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     7981 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     8041 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8101 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8161 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     8221 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     8281 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     8341 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     8401 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     8461 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     8521 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     8581 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     8641 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     8701 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     8761 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     8821 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     8881 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     8941 ggttcgtgca cacagcccag cttggagcga acgacctaca
//
