LOCUS       sequence-354745-       17243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with n-terminal fusion of full length 
            human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) 
            driven by EF-1alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17243)
  AUTHORS   Wang K, Escobar M, Li J, Mahata B, Goell J, Shah S, Cluck M, Hilton 
            IB
  TITLE     Systematic comparison of CRISPR-based transcriptional activators 
            uncovers gene-regulatory features of enhancer-promoter interactions.
  JOURNAL   Nucleic Acids Res. 2022 Aug 12;50(14):7842-7855. doi: 
            10.1093/nar/gkac582.
  PUBMED    35849129
REFERENCE   2  (bases 1 to 17243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-08-12- 12"; volume: "50"; issue: 
            "14"; pages: "7842-7855"
COMMENT     Sequence Label: GCN5 FL-dCas9-CBP core
FEATURES             Location/Qualifiers
     source          1..17243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(344..355)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             533..766
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(792..809)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    798..1022
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1068..1496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1155..1174)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1365..1386
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1505..1525)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1510..1839
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1690..1825
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1752..1771
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1887..1934
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1953..2327
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2457..2578
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2494..2513)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2548..2567
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2627..2643)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2627..2643)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2640..2662)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2651..2667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2675..2681,2682..2699,2700..2705))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2720..2741
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2858..2875)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3029..3617)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3109..3128)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3788..4579,4580..4648))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4411..4430
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4649..4753)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4828..4847)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5019..5398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5400..5598
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5553..5573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5616..5796
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5843..5968
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6461..6694
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6879..6923
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7221..7338
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        7470..7545
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7607..7818
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      7834..7843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10357..10377
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      10390..10399
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10396..10422,10423..10425,10426..12912,12913..12915,
                     12916..14499)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10396..14499
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             14500..14547
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      16171..16180
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16414..16437
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             16447..16503
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             16504..17100
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     16997..17017
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(17116..17243,1..461)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(17169..17189)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
       61 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
      121 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
      181 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
      241 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
      301 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
      361 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
      421 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
      481 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
      541 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
      601 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      661 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      721 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
      781 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
      901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
      961 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     1021 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     1081 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     1141 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1201 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1261 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1321 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1381 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1441 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     1501 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     1561 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     1621 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     1681 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     1741 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     1801 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     1861 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     1921 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     1981 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     2041 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     2101 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     2161 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     2221 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     2281 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     2341 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     2401 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     2461 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     2521 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     2581 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     2641 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2701 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2761 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2821 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2881 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2941 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3001 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3781 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3841 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3901 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3961 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4021 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4081 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4141 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4201 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4261 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4321 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4381 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4441 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     4801 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4861 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     4921 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     4981 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     5041 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     5101 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     5161 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     5221 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     5281 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     5341 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     5401 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     5461 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     5521 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     5581 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     5641 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5701 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5761 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     5821 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     5881 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     5941 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     6001 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     6061 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     6121 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     6181 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     6241 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     6301 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     6361 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     6421 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     6481 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     6541 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     6601 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     6661 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     6721 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     6781 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     6841 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     6901 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     6961 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     7021 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     7081 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     7141 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     7201 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     7261 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     7321 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     7381 aaggtaccga tatcactagt gttccattaa ctgcgctaaa aataattttt aaatcttttt
     7441 tagcttcttg ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata
     7501 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct
     7561 agctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
     7621 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga
     7681 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     7741 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     7801 tttgccgcca gaacacagga ccggttctag agcgccacca tggcggaacc ttcccaggcc
     7861 ccgaccccgg ccccggctgc gcagccccgg ccccttcagt ccccagcccc tgccccaact
     7921 ccgactcctg cacccagccc ggcttcagcc ccgattccga ctcccacccc ggcaccagcc
     7981 cctgccccag ctgcagcccc agccggcagc acagggactg gggggcccgg ggtaggaagt
     8041 gggggggccg ggagcggggg ggatccggct cgacctggcc tgagccagca gcagcgcgcc
     8101 agtcagagga aggcgcaagt ccgggggctg ccgcgcgcca agaagcttga gaagctaggg
     8161 gtcttctcgg cttgcaaggc caatgaaacc tgtaagtgta atggctggaa aaaccccaag
     8221 ccccccactg caccccgcat ggatctgcag cagccagctg ccaacctgag tgagctgtgc
     8281 cgcagttgtg agcacccctt ggctgaccac gtatcccact tggagaatgt gtcagaggat
     8341 gagataaacc gactgctggg gatggtggtg gatgtggaga atctcttcat gtctgttcac
     8401 aaggaagagg acacagacac caagcaggtc tatttctacc tcttcaagct actgcggaaa
     8461 tgcatcctgc agatgacccg gcctgtggtg gaggggtccc tgggcagccc tccatttgag
     8521 aaacctaata ttgagcaggg tgtgctgaac tttgtgcagt acaagtttag tcacctggct
     8581 ccccgggagc ggcagacgat gttcgagctc tcaaagatgt tcttgctctg ccttaactac
     8641 tggaagcttg agacacctgc ccagtttcgg cagaggtctc aggctgagga cgtggctacc
     8701 tacaaggtca attacaccag atggctctgt tactgccacg tgccccagag ctgtgatagc
     8761 ctcccccgct acgaaaccac tcatgtcttt gggcgaagcc ttctccggtc cattttcacc
     8821 gttacccgcc ggcagctgct ggaaaagttc cgagtggaga aggacaaatt ggtgcccgag
     8881 aagaggaccc tcatcctcac tcacttcccc aaattcctgt ccatgctgga ggaggagatc
     8941 tatggggcaa actctccaat ctgggagtca ggcttcacca tgccaccctc agaggggaca
     9001 cagctggttc cccggccagc ttcagtcagt gcagcggttg ttcccagcac ccccatcttc
     9061 agccccagca tgggtggggg cagcaacagc tccctgagtc tggattctgc aggggccgag
     9121 cctatgccag gcgagaagag gacgctccca gagaacctga ccctggagga tgccaagcgg
     9181 ctccgtgtga tgggtgacat ccccatggag ctggtcaatg aggtcatgct gaccatcact
     9241 gaccctgctg ccatgctggg gcctgagacg agcctgcttt cggccaatgc ggcccgggat
     9301 gagacagccc gcctggagga gcgccgcggc atcatcgagt tccatgtcat cggcaactca
     9361 ctgacgccca aggccaaccg gcgggtgttg ctgtggctcg tggggctgca gaatgtcttt
     9421 tcccaccagc tgccgcgcat gcctaaggag tatatcgccc gcctcgtctt tgacccgaag
     9481 cacaagactc tggccttgat caaggatggg cgggtcatcg gtggcatctg cttccgcatg
     9541 tttcccaccc agggcttcac ggagattgtc ttctgtgctg tcacctcgaa tgagcaggtc
     9601 aagggttatg ggacccacct gatgaaccac ctgaaggagt atcacatcaa gcacaacatt
     9661 ctctacttcc tcacctacgc cgacgagtac gccatcggct acttcaaaaa gcagggtttc
     9721 tccaaggaca tcaaggtgcc caagagccgc tacctgggct acatcaagga ctacgaggga
     9781 gcgacgctga tggagtgtga gctgaatccc cgcatcccct acacggagct gtcccacatc
     9841 atcaagaagc agaaagagat catcaagaag ctgattgagc gcaaacaggc ccagatccgc
     9901 aaggtctacc cggggctcag ctgcttcaag gagggcgtga ggcagatccc tgtggagagc
     9961 gttcctggca ttcgagagac aggctggaag ccattgggga aggagaaggg gaaggagctg
    10021 aaggaccccg accagctcta cacaaccctc aaaaacctgc tggcccaaat caagtctcac
    10081 cccagtgcct ggcccttcat ggagcctgtg aagaagtcgg aggcccctga ctactacgag
    10141 gtcatccgct tccccattga cctgaagacc atgactgagc ggctgcgaag ccgctactac
    10201 gtgacccgga agctctttgt ggccgacctg cagcgggtca tcgccaactg tcgcgagtac
    10261 aaccccccgg acagcgagta ctgccgctgt gccagcgccc tggagaagtt cttctacttc
    10321 aagctcaagg agggaggcct cattgacaag ggatccccca agaagaagag gaaggtggga
    10381 tccagcgctg ccaccatgga caagaagtac agcatcggcc tggccatcgg caccaactct
    10441 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
    10501 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
    10561 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
    10621 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
    10681 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
    10741 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
    10801 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
    10861 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
    10921 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
    10981 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
    11041 ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat
    11101 ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac
    11161 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg
    11221 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac
    11281 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc
    11341 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg
    11401 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc
    11461 aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc
    11521 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga
    11581 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac
    11641 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac
    11701 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg
    11761 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc
    11821 tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg
    11881 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
    11941 gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga
    12001 aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc
    12061 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc
    12121 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
    12181 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
    12241 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
    12301 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
    12361 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
    12421 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
    12481 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
    12541 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
    12601 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
    12661 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
    12721 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
    12781 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
    12841 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
    12901 tacgatgtgg acgccatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
    12961 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
    13021 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
    13081 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
    13141 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
    13201 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
    13261 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
    13321 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc
    13381 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
    13441 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
    13501 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
    13561 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
    13621 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
    13681 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
    13741 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac
    13801 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
    13861 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
    13921 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
    13981 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
    14041 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
    14101 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
    14161 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag
    14221 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
    14281 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
    14341 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
    14401 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
    14461 tacgagacac ggatcgacct gtctcagctg ggaggcgaca agcgacctgc cgccacaaag
    14521 aaggctggac aggctaagaa gaagaaagga tccatcttta aaccagagga gttacgccag
    14581 gccctcatgc caaccctaga agcactgtat cgacaggacc cagagtcatt acctttccgg
    14641 cagcctgtag atccccagct cctcggaatt ccagactatt ttgacatcgt aaagaatccc
    14701 atggacctct ccaccatcaa gcggaagctg gacacagggc aataccaaga gccctggcag
    14761 tacgtggacg acgtctggct catgttcaac aatgcctggc tctataatcg caagacatcc
    14821 cgagtctata agttttgcag taagcttgca gaggtctttg agcaggaaat tgaccctgtc
    14881 atgcagtccc ttggatattg ctgtggacgc aagtatgagt tttccccaca gactttgtgc
    14941 tgctatggga agcagctgtg taccattcct cgcgatgctg cctactacag ctatcagaat
    15001 aggtatcatt tctgtgagaa gtgtttcaca gagatccagg gcgagaatgt gaccctgggt
    15061 gacgaccctt cacagcccca gacgacaatt tcaaaggatc agtttgaaaa gaagaaaaat
    15121 gataccttag accccgaacc tttcgttgat tgcaaggagt gtggccggaa gatgcatcag
    15181 atttgcgttc tgcactatga catcatttgg ccttcaggtt ttgtgtgcga caactgcttg
    15241 aagaaaactg gcagacctcg aaaagaaaac aaattcagtg ctaagaggct gcagaccaca
    15301 agactgggaa accacttgga agaccgagtg aacaaatttt tgcggcgcca gaatcaccct
    15361 gaagccgggg aggtttttgt ccgagtggtg gccagctcag acaagacggt ggaggtcaag
    15421 cccgggatga agtcacggtt tgtggattct ggggaaatgt ctgaatcttt cccatatcga
    15481 accaaagctc tgtttgcttt tgaggaaatt gacggcgtgg atgtctgctt ttttggaatg
    15541 cacgtccaag aatacggctc tgattgcccc cctccaaaca cgaggcgtgt gtacatttct
    15601 tatctggata gtattcattt cttccggcca cgttgcctcc gcacagccgt ttaccatgag
    15661 atccttattg gatatttaga gtatgtgaag aaattagggt atgtgacagg gcacatctgg
    15721 gcctgtcctc caagtgaagg agatgattac atcttccatt gccacccacc tgatcaaaaa
    15781 atacccaagc caaaacgact gcaggagtgg tacaaaaaga tgctggacaa ggcgtttgca
    15841 gagcggatca tccatgacta caaggatatt ttcaaacaag caactgaaga caggctcacc
    15901 agtgccaagg aactgcccta ttttgaaggt gatttctggc ccaatgtgtt agaagagagc
    15961 attaaggaac tagaacaaga agaagaggag aggaaaaagg aagagagcac tgcagccagt
    16021 gaaaccactg agggcagtca gggcgacagc aagaatgcca agaagaagaa caacaagaaa
    16081 accaacaaga acaaaagcag catcagccgc gccaacaaga agaagcccag catgcccaac
    16141 gtgtccaatg acctgtccca gaagctgtat gccaccatgg agaagcacaa ggaggtcttc
    16201 ttcgtgatcc acctgcacgc tgggcctgtc atcaacaccc tgccccccat cgtcgacccc
    16261 gaccccctgc tcagctgtga cctcatggat gggcgcgacg ccttcctcac cctcgccaga
    16321 gacaagcact gggagttctc ctccttgcgc cgctccaagt ggtccacgct ctgcatgctg
    16381 gtggagctgc acacccaggg ccaggacgga tccgattaca aagacgatga cgataaggga
    16441 tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
    16501 ccgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
    16561 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
    16621 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
    16681 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
    16741 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
    16801 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
    16861 cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa gggtctgggc
    16921 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
    16981 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
    17041 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
    17101 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
    17161 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
    17221 gctattgctt cccgtatggc ttt
//
