LOCUS       sequence-354895-         389 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  It's an empty replication plasmid backbone  in Bacteroides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Zheng L, Tan Y, Hu Y, Shen J, Qu Z, Chen X, Ho CL, Leung EL, Zhao W,
            Dai L
  TITLE     CRISPR/Cas-Based Genome Editing for Human Gut Commensal Bacteroides 
            Species.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):464-472. doi: 
            10.1021/acssynbio.1c00543. Epub 2022 Jan 6.
  PUBMED    34990118
REFERENCE   2  (bases 1 to 389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00543"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "464-472"
COMMENT     Sequence Label: pB006
FEATURES             Location/Qualifiers
     source          1..389
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1..389
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 tgtcagccgt taagtgttcc tgtgtcactc aaaattgctt tgagaggctc taagggcttc
       61 tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag
      121 ccttatatat tctttttttt cttataaaac ttaaaacctt agaggctatt taagttgctg
      181 atttatatta attttattgt tcaaacatga gagcttagta cgtgaaacat gagagcttag
      241 tacgttagcc atgagagctt agtacgttag ccatgagggt ttagttcgtt aaacatgaga
      301 gcttagtacg ttaaacatga gagcttagta cgtgaaacat gagagcttag tacgtactat
      361 caacaggttg aactgctgat cttcagatc
//
