LOCUS       sequence-355012-       10230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid supports expression of 2 gRNAs targeting D.suzukii bTub, 
            Opie-mVenus tagged, and can be integrated with pBac..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   Kandul NP, Liu J, Buchman A, Shriner IC, Corder RM, Warsinger-Pepe 
            N, Yang T, Yadav AK, Scott MJ, Marshall JM, Akbari OS
  TITLE     Precision Guided Sterile Males Suppress Populations of an Invasive 
            Crop Pest
  JOURNAL   GEN Biotechnology (2022) 1:4, 372-385
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GEN 
            Biotechnology (2022) 1:4, 372-385"
COMMENT     Sequence Label: 1056D
FEATURES             Location/Qualifiers
     source          1..10230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2998..3060
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        5056..5475
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        5496..5571
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(6360..6362,6363..6365,6366..7079)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6405..6426)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6666..6685)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7013..7034
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7198..7319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7235..7254)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7289..7308
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7332..7751
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        7772..7847
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     repeat_region   9707..9741
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9923..9939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9923..9939)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9936..9958)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9947..9963
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9971..9977,9978..9995,9996..10001))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10016..10037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10154..10171)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggttta aacacccgta
     4081 ctttaagtta tcctgatttg gaaaaaaatt ttaacatttg aagtttttat tgtttagaaa
     4141 attcaatgga gtcgccccac atatgggaca gggttggctt tatctggtgc ctgtcagtgt
     4201 gtttaatact tttccatcta ttactgtcaa gtataaacta aagtttatat agaccgtttt
     4261 cccattttca taaacaaatt tggaagactg caaaatctta ttttgcttct ccaagtttgt
     4321 atgctccttt attaagcatt cctttgtttc ctttatgaat ttcaacttta gagacctgca
     4381 gaacctgata gattgtggaa atcaattgtt ccatagtata tttccgctag aatcaatcat
     4441 tcgtaaagga gtttctgctg tggcgaatat actgccatcg acatcacact tctgtggacc
     4501 tgcaaatccc tgtttgtagt tcgaatgacc tgagcttcca tcgtatccat aactaagtag
     4561 aagttcggcg attaaaaatg ttgtcccttg atctttcctt attttctaaa caactttttt
     4621 ttgtaaagtt aaaatccggc cagctgtgtg gttaagcaaa cttagcaaag gcacttctgc
     4681 ttttgtgtca ttcgttcttt ttgtttgcaa cttcgtagta cgaggaaaaa ggttgttttt
     4741 cgtttgatct ctaattgaga tattttgttg cttggttaaa ttttgatctt aaagaacagc
     4801 tagtgcgtcg ttaaatagta ttttctccaa tttcgcgagg ggctgactta ttaaggtgtg
     4861 catgctcttg gctttttcag ggcagatagc aaacagtttt ataatacatg ccagatctga
     4921 tttactgccc ttacgcgcag ccgcactagc tgaatttatt aataatttaa tatttccagc
     4981 cgtggatttg atcaaatttt aagctgctct tctctgagcc cttgctctcg acaacaaata
     5041 aggcaatgcg gccgcttttt gctcacctgt gattgctcct actcaaatac aaaaacatca
     5101 aattttctgt caataaagca tatttattta tatttatttt acaggaaaga attcctttta
     5161 aagtgtattt taacctataa tgaaaaacga ttaaaaaaaa tacataaaat aattcgaaaa
     5221 tttttgaata gcccaggttg ataaaaattc atttcatacg ttttataact tatgccccta
     5281 agtatttttt gaccatagtg tttcaattct acattaattt tacagagtag aatgaaacgc
     5341 cacctactca gccaagaggc gaaaaggtta gctcgccaag cagagagggc gccagtgctc
     5401 actacttttt ataattctca acttcttttt ccagactcag ttcgtatata tagacctatt
     5461 ttcaatttaa cgtcgcttga gtgtgcacca gctgggtttt agagctagaa atagcaagtt
     5521 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgcc
     5581 tacctggagc ctgagagttg ttcaataaaa taaaaatgtt tcgttttttt gctttcgcca
     5641 gtatttatta tttttcatca atatgtattc aatttggtat gtatttagta attgtaatat
     5701 atagacaatg gttttccgtt gacgtacata catctgacgt gtgtttattt agacataata
     5761 gttatgtttt cacatctttt taatgttcgc ttaatgcgta tgcattaatt aacatactcg
     5821 gtggcctccc caccaccaac ttttttgcac tgcaaaaaaa cacgcttttg cacgcgggcc
     5881 catacatagt acaaactcta cgtttcgtag actattttac ataaatagtc tacaccgttg
     5941 tatacgctcc aaatacacta ccacacattg aacctttttg cagtgcaaaa aagtacgtgt
     6001 cggcagtcac gtaggccggc cttatcgggt cgcgtcctgt cacgtacgaa tcacattatc
     6061 ggaccggacg agtgttgtct tatcgtgaca ggacgccagc ttcctgtgtt gctaaccgca
     6121 gccggacgca actccttatc ggaacaggac gcgcctccat atcagccgcg cgttatctca
     6181 tgcgcgtgac cggacacgag gcgcccgtcc cgcttatcgc gcctataaat acagcccgca
     6241 acgatctggt aaacacagtt gaacagcatc tgttacagcg acacaacatg agccggtcca
     6301 acaacgccaa cgcgcccacg ccatccaacc gccgccgcaa cctgtctctg gtggctagca
     6361 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     6421 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     6481 gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     6541 tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     6601 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     6661 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     6721 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     6781 agctggagta caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg
     6841 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg
     6901 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     6961 acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     7021 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     7081 cgctgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     7141 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     7201 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7261 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     7321 agcttcttaa gtttttgctc acctgtgatt gctcctactc aaatacaaaa acatcaaatt
     7381 ttctgtcaat aaagcatatt tatttatatt tattttacag gaaagaattc cttttaaagt
     7441 gtattttaac ctataatgaa aaacgattaa aaaaaataca taaaataatt cgaaaatttt
     7501 tgaatagccc aggttgataa aaattcattt catacgtttt ataacttatg cccctaagta
     7561 ttttttgacc atagtgtttc aattctacat taattttaca gagtagaatg aaacgccacc
     7621 tactcagcca agaggcgaaa aggttagctc gccaagcaga gagggcgcca gtgctcacta
     7681 ctttttataa ttctcaactt ctttttccag actcagttcg tatatataga cctattttca
     7741 atttaacgtc gtgttctgga tgtggtgcga agttttagag ctagaaatag caagttaaaa
     7801 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgcctacc
     7861 tggagcctga gagttgttca ataaaataaa aatgtttcgt ttttttgctt tcgccagtat
     7921 ttattatttt tcatcaatat gtattcaatt tggtatgtat ttagtaattg taatatatag
     7981 acaatggttt tccgttgacg tacatacatc tgacgtgtgt ttatttagac ataatagtta
     8041 tgttttcaca tctttttaat gttcgcttaa tgcgtatgca tctcgagcct atctatgcaa
     8101 atctcagtgt gggatgtaag ccaagttgag tactttttct tgaaatgatt aaaatttctt
     8161 ttgcaatatg tctatttgcg ggacaatcta taacaaaaag tttcgctttg tttttttaaa
     8221 acatcctcat aagcatctga aacagctatt gaagctctaa atctgtcaaa tattgcctgc
     8281 tgtttctcac gctttttctc tccagaactc caagtctcaa acaattctga attggccaac
     8341 ctgaggttgt gacctaaaaa tgtatatttt agtgttagtt tttattgtta atttgtacac
     8401 gtaaagcggc aattaaaagc actcaacaca aacaaatcca acttgtgacg accacaaaca
     8461 tatagtacga accgctctct ttcgatgcca tgacaacttg tttgatataa acttagattg
     8521 gtgctttatt tttaataatt ttaaacggac tcttttttcc gcagagtagc tttcgtgcac
     8581 ccgtgatacc gaacagctgt tttaacagcc tatatatttt aacagatcgt gactcagata
     8641 aaatttctgt tacgtaacat tgaagtttga ttttaatatt aacgactttc aaaaaatgga
     8701 tttttgccca caaaacgtga tcagccagcc taaagtgcac tcgctgcaaa taggaccgca
     8761 cgcgtaatgt attttagtat tttctacgag ttaaattaat ttttgtattt gacgtttcgg
     8821 gatgttttta aaaaaggtag ttgttgttgc ggcagtaccc agtagccttc cagcggctcg
     8881 aaactgaaca tttgatctca tcctgaaaag aaatattgct atgctcattt ctaagaaaat
     8941 gaccaacctc catcttgtgt cctgcttggc aagactttgc acctgttgca ccaaaagtac
     9001 attattttca ctacgcttgc ttgaatagaa ttctcatcgg cgcgcctagg ccggccgatc
     9061 tcggatctga caatgttcag tgcagagact cggctacgcc tcgtggactt tgaagttgac
     9121 caacaatgtt tattcttacc tctaatagtc ctctgtggca aggtcaagat tctgttagaa
     9181 gccaatgaag aacctggttg ttcaataaca ttttgttcgt ctaatatttc actaccgctt
     9241 gacgttggct gcacttcatg tacctcatct ataaacgctt cttctgtatc gctctggacg
     9301 tcatcttcac ttacgtgatc tgatatttca ctgtcagaat cctcaccaac aagctcgtca
     9361 tcgctttgca gaagagcaga gaggatatgc tcatcgtcta aagaactacc cattttatta
     9421 tatattagtc acgatatcta taacaagaaa atatatatat aataagttat cacgtaagta
     9481 gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat
     9541 aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc
     9601 attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc
     9661 gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt
     9721 acgcagacta tctttctagg gttaaaaaag atttgcgctt tactcgacct aaactttaaa
     9781 cacgtcatag aatcttcgtt tgacaaaaac cacattgtgg ccaagctgtg tgacgcgacg
     9841 cgcgctaaag aatggcaaac caagtcgcgc gagcgtcgac tctagaggat ccccgggtac
     9901 cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     9961 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10021 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10081 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    10141 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    10201 tatcagctca ctcaaaggcg gtaatacggt
//
