LOCUS       sequence-355070-       13792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All in one ""tet-on"" lentivirus vector that expresses rhesus 
            cytomegalovirus Rh159 fused to an eGFP tag at its cytoplasmic tail. 
            Rh159 is an ER resident protein that binds NKG2D activating 
            ligands..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13792)
  AUTHORS   Zhang H, Read C, Nguyen CC, Siddiquey MNA, Shang C, Hall CM, von 
            Einem J, Kamil JP
  TITLE     The Human Cytomegalovirus Nonstructural Glycoprotein UL148 
            Reorganizes the Endoplasmic Reticulum.
  JOURNAL   mBio. 2019 Dec 10;10(6). pii: mBio.02110-19. doi: 
            10.1128/mBio.02110-19.
  PUBMED    31822584
REFERENCE   2  (bases 1 to 13792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2019-12-10"; volume: "10(6). pii: mBio.02110-19. doi"; pages:
            " 10.1128/mBio.02110-19"
COMMENT     Sequence Label: pOUPc-Rh159-GFP
FEATURES             Location/Qualifiers
     source          1..13792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      60..69
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(73..91)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(83..130)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        179..557
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    259..277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    295..313
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    331..349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    367..385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    403..421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      563..572
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1547..1549,1550..1552,1553..2263)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        2281..3492
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3329..3348
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3499..3532
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(3539..4156,4157..4162,4163..4198,4199..4201,4202..4237,
                     4238..4240,4241..4276,4277..4285)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4334..4906
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4501..4518)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4728..4747
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4909..5511
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5408..5428
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5533..6121
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5586..5606)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6004..6015)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6328..6561
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6662..6679)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6668..6892
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6938..7366
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7025..7044)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7235..7256
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7375..7395)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7380..7709
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7560..7695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7622..7641
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    8532..8653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8702..8718)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8702..8718)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8715..8737)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8726..8742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8750..8756,8757..8774,8775..8780))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8795..8816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8933..8950)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9104..9692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9184..9203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9863..10654,10655..10723))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10486..10505
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10724..10828)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10876..11010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             11178..11811
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11858..11983
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12539..12772
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12957..13001
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13299..13416
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(join(13481..13792,1..63))
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg tcaacttggc
       61 catggtggcc ctcctatagt gagtcgtatt atactatgcc gatatactat gccgatgatt
      121 aattgtcaac acgtgctgca ggtccgaggt tctaggctag cgagactagc gacgagttgg
      181 ctttactccc tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag
      241 aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta
      301 tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca
      361 gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag
      421 aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc aggtagtgaa
      481 ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact ttccgtacca
      541 cttcctaccc tcgtaaaccg gtgccaccat ggcctacaac agcttccttc tcagctgcct
      601 taccatcgca cttttactgc acggtcttcc aacatcaact agcgctaaag acatggcagg
      661 ctacgtacac gttcatatgc ttaacgcacc tgaagacgcc gcttctacgt ctgcgccagt
      721 tatccaacta gccaacggct ttattacctg cagaaaattt acagagctcg gttgggcact
      781 gtgtactagc tgggatgata tggagtgttt tccgttttgg cgaaggactt tctcggaaac
      841 taaaaatatc actgtgcttt cagacgatcc atactatgcc ggcgttgtgt tgacagtacg
      901 gacgcttctt ggtgactccc tagagcttac atcttatctc ggggtcaggc cttcgattgc
      961 caatgtgggc tactatgtaa tgtacctgtc atatgctaag gcgctcccgg caaaatttac
     1021 ccacaacgat gtggactatg tgaggtttgc catatacctt cacaccgaaa ttgatgcgca
     1081 gcaaactcct gacaacaact tcgtctttac gttcgccaca cccgggacat ttgaaacccc
     1141 agacctcatg agtagttttg tgattggaac cgatgatgga caaaatagca ccttgaattt
     1201 caccactgtt ggcggagaaa aacaccgcta caacaacgtg gagctcagct cagaaatgaa
     1261 gggcaattgt gtgtatcact cggctattat ccggaacgtc gccagctcgc aatcttgggt
     1321 gtcgtttgac ttctccctat attggcgacg taccaaaatt gcatatagtt acgtgcagta
     1381 ttatttcccg aaaccactcc attcacctga acccataggt cctgcctggc agagaatcta
     1441 ccattacttt agcctcctga cgcagcttcg ctacggaaca gtagcgatca ccgtggtgct
     1501 cattttcctt ataggtgcac ttctaaaacg cagtcgtgaa gctcatatgg tgagcaaggg
     1561 ggaggagctt tttactggcg ttgttcccat cctggttgag ttggatgggg atgtcaacgg
     1621 ccacaagttc agcgtttctg gagaaggaga aggcgacgca acctatggga agctcacttt
     1681 gaaattcata tgcaccaccg gtaaactccc agtcccctgg cctaccctgg tcactacctt
     1741 gacatatgga gttcagtgct tttctcgata ccccgaccac atgaagcagc acgatttttt
     1801 caaatctgca atgcccgagg gctatgtaca agagaggacc attttcttca aagacgacgg
     1861 caattacaag accagggccg aggtcaaatt tgaaggggac actcttgtga atcggatcga
     1921 acttaaaggg atcgatttca aagaggacgg aaacatactg gggcataagc tggaatacaa
     1981 ctacaatagc cataatgttt acattatggc agacaagcag aaaaacggta ttaaggtcaa
     2041 cttcaagata cggcataaca ttgaagatgg gtcagtccag cttgctgacc attatcagca
     2101 gaatacaccc attggcgatg gtcccgtgct tttgcctgac aaccactacc tttctaccca
     2161 aagcgcattg tcaaaagatc ctaacgagaa gagagatcat atggtccttt tggagttcgt
     2221 aaccgcagct ggtataactc tgggtatgga cgagctgtat aagtaaacgc gttaagatct
     2281 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
     2341 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
     2401 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
     2461 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
     2521 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
     2581 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
     2641 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
     2701 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
     2761 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
     2821 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
     2881 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
     2941 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
     3001 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
     3061 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
     3121 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
     3181 tggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
     3241 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     3301 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     3361 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     3421 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     3481 tttttgttag acggatccat aacttcgtat aatgtatgct atacgaagtt atgccaccat
     3541 gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg
     3601 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
     3661 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga
     3721 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
     3781 caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
     3841 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
     3901 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
     3961 actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc
     4021 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg
     4081 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
     4141 aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc
     4201 agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt
     4261 tgaccttgac atgctccccg ggtaaataac ttcgtatagc atacattata cgaagtatgg
     4321 cgcgccaatt ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     4381 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     4441 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     4501 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     4561 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     4621 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     4681 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     4741 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     4801 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     4861 ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat ggccaccgag
     4921 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc
     4981 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc
     5041 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5101 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     5161 gcgggggcgg tgttcgccga gatcggctcg cgcatggccg agttgagcgg ttcccggctg
     5221 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     5281 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     5341 gtgctccccg gagtggaggc ggccgagcgc gctggggtgc ccgccttcct ggagacctcc
     5401 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     5461 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg attaattaag
     5521 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5581 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5641 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5701 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5761 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5821 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5881 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     5941 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6001 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6061 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6121 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata
     6181 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     6241 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     6301 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     6361 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6421 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6481 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6541 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     6601 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgtttaaac ccgctgatca
     6661 gcctcgactg tgccttctag ttgccagcca tctgttgttt gccccttccc cgtgccttcc
     6721 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6781 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6841 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6901 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6961 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7021 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7081 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7141 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7201 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7261 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7321 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7381 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7441 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7501 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7561 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7621 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7681 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7741 gatctgatca gcacgtgatg aaaaagcctg aactcaccgc gacgtgaccg caaggaatcg
     7801 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     7861 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     7921 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     7981 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     8041 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     8101 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     8161 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     8221 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     8281 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     8341 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     8401 agcacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8461 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8521 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8581 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactaa
     8641 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     8701 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8761 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8821 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8881 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8941 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9001 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9061 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9361 actaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9541 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9781 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9841 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9901 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9961 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10021 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10081 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10141 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10201 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10261 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10321 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10381 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10441 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10501 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10561 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10621 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10681 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10741 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10801 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    10861 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10921 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10981 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    11041 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    11101 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    11161 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
    11221 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
    11281 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
    11341 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
    11401 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
    11461 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
    11521 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
    11581 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
    11641 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    11701 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    11761 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
    11821 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
    11881 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    11941 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    12001 aattagatcg cgatgggara aaawkmkgkk wrsgmsagcg tcagtattaa gcgggggaga
    12061 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    12121 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    12181 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    12241 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    12301 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    12361 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
    12421 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
    12481 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
    12541 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
    12601 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
    12661 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
    12721 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
    12781 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
    12841 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
    12901 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
    12961 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
    13021 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
    13081 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
    13141 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
    13201 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
    13261 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
    13321 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
    13381 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
    13441 gatccagttt ggttagtacc gggcccgctc tagtccggaa tcagtcctgc tcctcggcca
    13501 cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct
    13561 cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg
    13621 accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg
    13681 gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg
    13741 cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag aa
//
