LOCUS       sequence-355091-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CG3
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     354..370
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     354..370
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     375..394
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        375..393
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(568..1758)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1387..1406
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1806..1811,1812..1828,1829..1834))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1961..2549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2041..2060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actcacgtta agggattttg gtcatgggtg gctcgaccag atatggtctc actacaactt
       61 gctccggagt cagaccgtgg gcttgacaga gaactggcag taggcgctgt acggtttcaa
      121 gagcctgttt gccaccattg ttactagcaa tcgctacaac ttgcgctggg gttagtccgt
      181 gggcttgaca gagaactggt agaaggcgtt ggacggtttc aagagcctgt ttgccaccgt
      241 catgactagc aatcgctacc acgagaccag ctttggtcta gaggaggcat gatgatatat
      301 ttttatcttg tgcaatgtaa catcagagat tttgagacac gggccagagc tgccaggaaa
      361 cagctatgac catgtaatac gactcactat aggggatatt ttattttctc ccaatcaggc
      421 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat caagggttgg
      481 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
      541 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
      601 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
      661 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt
      721 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
      781 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
      841 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
      901 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
      961 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     1021 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     1081 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     1141 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatgggtc ggcgctctcc
     1201 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     1261 cgccgcaagg aatggtgcat gtaaggagat ggcgcccaac agtcccccgg ccacggggcc
     1321 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     1381 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     1441 ggccacgatg cgtccggcgt agagaatcca caggacgggt gtggtcgcca tgatcgcgta
     1501 gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga
     1561 cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac
     1621 gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac
     1681 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag
     1741 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact
     1801 accgcattaa agctaatcga tgataagctg tcaaacatga gaattaattc atgttctttc
     1861 ctgcgttatc ccctgattct gtggataacg caggaaagaa catgtgagca aaaggccagc
     1921 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1981 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2041 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2101 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2161 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2221 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2281 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2341 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2401 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2461 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2521 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2581 acgctcagtg gaacgacgcg ta
//
