LOCUS       sequence-355126-        4410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC7A3_STOP
FEATURES             Location/Qualifiers
     source          1..4410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1967..2066)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2083..2102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2084..2102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2107..2123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2107..2123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2225..2244)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2236..3045
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2303..2322)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3192..3780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3681..3700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3934..3951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4110..4137)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4229..4315)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4364..4386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4378..4395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4379..4395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgcc ttggcaggcc
      121 ttccggagat ttggccagaa gctggtgagg cgccggacac tggagagcgg aatggcagag
      181 accagactgg ccaggtgcct gtccacactg gatctggtgg ccctgggagt gggatctacc
      241 ctgggagcag gcgtgtatgt gctggcagga gaggtggcaa aggacaaggc aggcccctcc
      301 atcgtgatct gctttctggt ggccgccctg agctccgtgc tggcaggact gtgctacgca
      361 gagttcggag caagggtgcc tcggtccgga tctgcctacc tgtattctta cgtgaccgtg
      421 ggcgagctgt gggcctttac cacaggctgg aacctgatcc tgtcttatgt gatcggaaca
      481 gcaagcgtgg caagggcatg gtctagcgcc ttcgataacc tgatcggcaa tcacatcagc
      541 aagaccctgc agggatccat cgccctgcac gtgccacacg tgctggcaga gtaccctgac
      601 ttctttgcac tgggactggt gctgctgctg acaggactgc tggccctggg agcaagcgag
      661 tccgccctgg tgacaaaggt gtttaccggc gtgaatctgc tggtgctggg ctttgtgatg
      721 atctccggct tcgtgaaggg cgacgtgcac aactggaagc tgaccgagga ggattatgag
      781 ctggccatgg ccgagctgaa tgacacatac tctctgggac cactgggaag cggaggattc
      841 gtgcccttcg gctttgaggg aatcctgagg ggagcagcaa cctgcttcta tgcctttgtg
      901 ggcttcgatt gtatcgcaac cacaggagag gaggcacaga acccacagcg gagcatccct
      961 atgggcatcg tgatctctct gagcgtgtgc tttctggcct acttcgccgt gtcctctgcc
     1021 ctgaccctga tgatgcctta ctatcagctg cagccagagt ccccactgcc tgaggccttt
     1081 ctgtatatcg gatgggcacc tgcaagatac gtggtggccg tgggcagcct gtgcgccctg
     1141 tccacatctc tgctgggctc catgtttcca atgcccagag tgatctacgc catggccgag
     1201 gatggactgc tgttccgcgt gctggcaagg atccacaccg gaacaaggac cccaatcatc
     1261 gccaccgtgg tgtctggcat catcgccgcc ttcatggcct tcctgtttaa gctgaccgac
     1321 ctggtggatc tgatgagcat cggcacactg ctggcctata gcctggtgtc catctgcgtg
     1381 ctgatcctgc ggtaccagcc agaccaggag acaaagaccg gagaggaggt ggagctgcag
     1441 gaggaggcca tcaccacaga gagcgagaag ctgaccctgt ggggcctgtt ctttcccctg
     1501 aattccatcc ctacaccact gtctggccag atcgtgtacg tgtgcagctc cctgctggcc
     1561 gtgctgctga ccgccctgtg cctggtgctg gcacagtggt ccgtgccact gctgtctggc
     1621 gacctgctgt ggacagccgt ggtggtgctg ctgctgctgc tgatcatcgg catcatcgtg
     1681 gtcatctgga gacagccaca gtctagcacc cccctgcact ttaaggtgcc cgccctgcct
     1741 ctgctgccac tgatgagcat cttcgtgaac atctatctga tgatgcagat gacagcagga
     1801 acctgggcaa ggttcggcgt gtggatgctg atcggctttg ccatctattt cggctacggc
     1861 atccagcaca gcctggagga gatcaagtcc aatcagcctt ctagaaagag cagggccaag
     1921 acagtggacc tggatccagg caccctgtac gtgcactccg tgtaagaccc agctttcttg
     1981 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     2041 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     2101 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     2161 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2221 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     2281 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     2341 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     2401 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     2461 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2521 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2581 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2641 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     2701 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2761 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2821 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2881 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2941 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     3001 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     3061 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     3121 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     3181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3301 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3541 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3661 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3721 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3781 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3841 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3901 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3961 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4021 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     4081 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     4141 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     4201 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     4261 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     4321 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     4381 aaaacgacgg ccagtcttaa gctcgggccc
//
