LOCUS       sequence-35527-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pDONR223-BRAF V600E
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagttggc aagtggacag
      121 gaaacgcacc atatccccct gcctggatgg gtgtttttgg agaagcacaa gcatatagac
      181 taaaatcctc tgtttggaaa ccagcccgat tcaaggaggg ttctgatgca ctgcggtgaa
      241 tttttggcaa tgagcgggcc agcagctcaa tagaggcgag aatttgggga aagagtggtc
      301 tctcatctct tttctttttg aggcactctg ccattaatct cttcatggct tttggacagt
      361 tactccgtac cttactgaga tctggagaca ggtatcctcg tcccaccata aaaattatct
      421 ggtccctgtt gttgatgttt gaataaggta actgtccagt catcaattca tacagaacaa
      481 ttccaaatgc atatacatct gactgaaagc tgtatggatt tttatcttgc attctgatga
      541 cttctggtgc catccacaaa atggatccng acaactgttc aaactgatgg gacccactcc
      601 atcgagattt ctctgtagct agaccaaaat cacctatttt tactgtgagg tcttcatgaa
      661 gaaatatatt attactcttg aggtctctgt ggatgattga cttggcgtgt aagtaatcca
      721 tgccctgtgc agtctgtcgt gcaatatcta taagtttgat catctcaaat ttggtctcaa
      781 tgatatggag atggtgatac aagctggagc cctcacacca ctgggtaaca atagccagtt
      841 gtggctttgt ggaatagccc
//
