LOCUS       sequence-355283-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CG9
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     529..545
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     529..545
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     550..569
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        550..568
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(743..1933)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1562..1581
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1981..1986,1987..2003,2004..2009))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2136..2600,1..124))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2216..2235)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
       61 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
      181 cacgttaagg gattttggtc atgggtggct cgaccagata tggtctcgtt cccatgacgg
      241 tggcaaacag gctcttgaaa ccgtccaacg ccttctacca gttctctgtc aagcccacgg
      301 actaacccca gcgcaagttg tagcgattgc tagtaacaat ggtggcaaac aggctcttga
      361 aaccgtacag cgcctactgc cagttctctg tcaagcccac ggtctgactc cggagcaagt
      421 tgtagcgatt gcctcttgag accagctttg gtctagagga ggcatgatga tatattttta
      481 tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgcca ggaaacagct
      541 atgaccatgt aatacgactc actatagggg atattttatt ttctcccaat caggcttgat
      601 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatcaagg gttggtttgc
      661 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta
      721 gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca
      781 acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg
      841 tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccaatgatc gaagttaggc
      901 tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg
      961 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg
     1021 ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg
     1081 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1141 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1201 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1261 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1321 accggaagga gctgactggg ttgaaggctc tcaagggcat gggtcggcgc tctcccttat
     1381 gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg
     1441 caaggaatgg tgcatgtaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     1501 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     1561 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     1621 cgatgcgtcc ggcgtagaga atccacagga cgggtgtggt cgccatgatc gcgtagtcga
     1681 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg
     1741 ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat
     1801 agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca
     1861 taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat
     1921 tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc
     1981 attaaagcta atcgatgata agctgtcaaa catgagaatt aattcatgtt ctttcctgcg
     2041 ttatcccctg attctgtgga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2101 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2161 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2221 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2281 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2341 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2401 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2461 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2521 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2581 gtatttggta tctgcgctct
//
