LOCUS       sequence-355314-        2608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CT1
FEATURES             Location/Qualifiers
     source          1..2608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     363..382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(589..594,595..611,612..617)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             665..1855
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1017..1036)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2029..2048)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2030..2048)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2053..2069)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2053..2069)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2482..2608,1..462)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
       61 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      121 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      181 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      241 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      301 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      361 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      421 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      481 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      541 acagaatcag gggataacgc aggaaagaac atgaattaat tctcatgttt gacagcttat
      601 catcgattag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc
      661 gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt
      721 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga
      781 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg
      841 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc
      901 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggattct
      961 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     1021 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     1081 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     1141 acatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     1201 cctaatgcag gagtcgcata agggagagcg ccgacccatg cccttgagag ccttcaaccc
     1261 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     1321 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
     1381 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     1441 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
     1501 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     1561 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     1621 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
     1681 gctcgcggct cttaccagcc taacttcgat cattggaccg ctgatcgtca cggcgattta
     1741 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
     1801 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
     1861 agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
     1921 cggagaactg tgaatgcgca aaccaaccct tgatcgggga agaacagtat gtcgagctat
     1981 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatatccc ctatagtgag
     2041 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     2101 tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccaa agctggtctc
     2161 gccccggagc aggtggtggc catcgctagt catgacggtg gcaaacaggc tcttgaaacc
     2221 gtccaacgcc ttctaccagt tctctgtcaa gcccacggac taaccccagc gcaagttgta
     2281 gcgattgcta gtaatggggg tggcaaacag gctcttgaga ctgttcagcg ccttctacca
     2341 gttctctgtc aagcccacgg cctgacgccc gagcaggtga gaccatatct ggtcgagcca
     2401 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     2461 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2521 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2581 ttttccgaag gtaactggct tcagcaga
//
