LOCUS       sequence-355457-        9004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-4::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression
            of UNC-7-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 9004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH2165
FEATURES             Location/Qualifiers
     source          1..9004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(3866..3877)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     primer_bind     complement(6529..6551)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6651..6670
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6748..6766)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6834..6938
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6939..7007,7008..7799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7157..7176)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7970..8558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8459..8478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8712..8729
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8846..8867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8882..8887,8888..8905,8906..8912)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8920..8936
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8925..8947
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8944..8960
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8944..8960
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca caacgatgga
       61 tacgctaaca acttggaaat gaaataagct tgggctgcag catgcggccg cttccccaaa
      121 ttggaacagt gaaatactca atcaatcact ctggcggttt tctccacaaa gaacacgaaa
      181 ttggaaaagg gaaaagattg atttgtttga aggcgtgagg aaatcgaaaa aaaaatgttt
      241 caaaacaaat gaaatatttt aatgaataaa cccaggaatg ccaccttttg gtgcccgcct
      301 acaaaaaagt caactgttct taaactccta catagttgcc ctctctaacg aatgttgctt
      361 tttttaaatt ttcattgaat ttgattatcg tctgtatgcg cctactctat cagtggaaaa
      421 catgcccatt tcccacaaat ttactcgttt ggagctcaaa atgttaaatg gggcatcatg
      481 gaattggcat ctcacagaaa gtggcggatg agttatatgt aataataatg gtggctgaag
      541 tggaaaccgc aaaaaaagga tgtgtatttg agattttgta acggaatttg aatcagatac
      601 acaaaatgag agaaatttat agattcttta aaatccacct gaagtcggca aatttttgaa
      661 aggtctgttc gttcagtttt tcataagttt tatgaatttt gatttactac tgaagttcga
      721 gaaaagccct ttaaccatac caataagaaa ttatgttaac tttacaagaa tattaacaaa
      781 aaattattaa aaaactgtgt tcccctggca ttatgatatt tccaccgtca gttctctatt
      841 aaaacttcaa ctctacacag tttcaactct caaatgagct gtccatcttt tgccttcagg
      901 aaattatgct aatctgctga tttcgcgcac tttcaccaca ctatttccaa atagaaggag
      961 gggtggtgca acacacgaga cactgcaaaa aggttcagtg aagatttatc tacttgtgag
     1021 atgagagcag gaggggtggg agaagtgtga tgattgacgg gatgacaaga acagtgatgt
     1081 ggtgtcgaaa tggaagatgc gctaatttgt ggggttttat gaaatttgaa taattacatt
     1141 tataacatct actagttagg cgtctgtgta ggtattgcct gcctgcctac aaggcatatg
     1201 tacgaggcag gttaaacctc ctagaaagct acggtacttt caaaccacat aacatttata
     1261 ctggtgtctt ttcgcgtctt cttcttgaac atgtagaccc tcaaaaagct aagttttaca
     1321 ctgtgtagtt tgctttgaaa tctacaaaat tggaggcacg ccttctggac tttgccgaca
     1381 cgcatacttt ctacctacca actgcctact tccacagtct agcgtgaact ccaataccat
     1441 tttaaaaatt gttttatctc aaattttcaa tttttaaatc caatttccca gttctaaaaa
     1501 aaaaattttt tttttaattt aaaaactctc atccttttta atgctcgttc ccattcggca
     1561 agacgtgtac caaacacaaa acaaattgac tggtgatcca tctcgaattt ctcgtttcaa
     1621 attagtcgtt gctatatata ttgtcttatg tgtggaaaac gaataagaaa aattagagaa
     1681 agatgagaga gcaggggcag aatgaaaatt tgcatggaaa ttgaagaaga tcactgcttt
     1741 ttccgatgaa tgatgactaa atagaatgag ttgagaggaa aataatcata attctaattt
     1801 taattagaaa tatgatatgg aaagtaagca aaaccacaag gcttttgctc tgaacttatt
     1861 tccgaaaaaa aaaagtgaaa ctgaaagtta gaattttcaa aataatattg cgagagtcaa
     1921 ccttcagaaa aattggcgag aaatcaaatt tgaattctct gaaaatttag ttaaaataca
     1981 tatgttatcc ttctttgttt tttggcctta gcctgccttc aaggcgacct gagcctatgc
     2041 gggtgcctaa taccgaaaca cacataattt ttttttttaa tctttaaata ctgtaacgag
     2101 tcgcgagatc cagcgagacc catagaatat gttgagcgct gcaaacaatc gtataactct
     2161 tgttcaaatt tttccactct gtatttttgc aagcagttca acaaatggtc atcattttta
     2221 taacccatat ctatattcga atctgacata acaaataata ctaagcacat cctcccacat
     2281 ctcttttcat tcaagttgcg caattcccct aataactatg gatggagggg ggaccgagcc
     2341 caaagattat tcaaaacgat aacgactttt tcatgcggcc aacgaaaaat aaaacttttc
     2401 tcatattctc ctatttccca tttcaagaat tacatcccca tgggacgttg aaatcacatc
     2461 atattagtat atgttaatag ttcttttcac tggaatatcg ccacgccccc cttccggtgg
     2521 ctcattggtt tcgagaagaa ggcggagccg aagtgggttc ttctttcttt ttttgtggta
     2581 gaagtctagc aggattaagc tactattcca ttagaggatc ttcttcttcc aaaaagtgaa
     2641 aatcccggga ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag
     2701 aacattttca ggaggaccct tggctagcgt cgacggatcc ccatgctcgg ctcctccagc
     2761 aatcctgaac cgccactcct gtcgcggata atcggagtac ctccaccgcc gccgccacga
     2821 gcaccgacca cagctctcgt ccttcgggtg cccaccgtcg acagtccatc gaaaaagaaa
     2881 caacagccag acactaggaa caaatatcag gaaactgcgc tcagggataa aaaaactcgg
     2941 acaccacttg aaaaagccag acatctcgac aatcttccct cgtatcaggc ccaaaaacta
     3001 ttagacggtt cgcaccagct tcgcatcgac tcccaccacg ttggtagtgc aggtcacggt
     3061 gcaggacaag gtcacggaca caagaaagag tttggacctg caatgattct ctattattta
     3121 gcttctgctt ttcgggcgct ctatccgcgt ctagatgatg attttgtgga caaacttaat
     3181 tattattata ctactacaat attagcttcc tttgcacttc ttgtttccgc caagcagtat
     3241 gtcggttttc ccatccaatg ttgggtgccc gccacattca cagatgccat ggagcagtac
     3301 actgagaact actgctgggt acagaacaca tactgggttc ccatgcaaga ggacattcca
     3361 cgtgaaattt attcgcgtag aaatcgacaa attggctatt accaatgggt gccgtttatt
     3421 cttgctattg aggccttgtt gttctacgtt ccatgcattc tctggagagg attgttgtat
     3481 tggcacagtg gtatcaacct acaagggcta gtccagatgg cgtgtgacgc acgactgatg
     3541 gattcggaaa taaagacgag aacagtctac acaatggccc ggcacatgca agacgaggtt
     3601 cagctcacca atatcgaccg gcaaggacac agtcggagtt gtttctcgaa tttgcaactt
     3661 ggcgcaaact gtgggagaca ttgtggttgc tacgtcacta tgctctacat aggaatcaag
     3721 gttctctact cagcaaatgt gttgctccag tttttcttgc tgaaccatct tcttgggtct
     3781 aacgacttgg cttatggatt ctctttgttg aaagacttga tgcacgcgat tgagtgggaa
     3841 cagactggaa tgttccccag ggtcacactt tgtgattttg aggttcgagt tctgggaaac
     3901 attcatcgac ataccgttca gtgcgtgtta atgatcaata tgtttaatga gaaaattttc
     3961 ttattcctct ggttttggtt cttaacctgt ggaattgtta ccgtatgtaa cactatgtac
     4021 tggattctca ttatgttcat tccgtctcag ggaatgtcat ttgtccgcaa atacctccgt
     4081 gttctacctg accaccctgc gaaaccaata gctgatgacg tcacgctgag gaagttcact
     4141 aataacttcc tccgcaaaga tggagtcttc atgttgcgaa tgatttccac acacgccggc
     4201 gaactgatga gcagtgagct cattcttgct ctctggcagg atttcaacaa cgtcgaccgc
     4261 agtccgacgc aattttggga tgcggaacac ggtcaaggga cgatagactg acggatcccc
     4321 gagatcttca gcgctcagca ttcgtagaat tgtaagttta aactttctca tactaactaa
     4381 cgcgctctat ttaaattttc agaattcgct gtctcatcct actttcacct agttaactgc
     4441 ttgtcttaaa atctatgctt ctctttagta tctaaaattt tcctagaagc ttacaagtat
     4501 ataaatggtc tcttctcaat aaaggttgta tatttattca tcttattgaa tctgccattt
     4561 cctcgttttt gcgagtttat ataccttcca attttctttc tattgtattt tcaacttcta
     4621 attttaattc agggaaactg cttcaacgca tcggtaccct atggtctcaa agggtgaaga
     4681 agataacatg gcaattatta aagagtttat gcgtttcaag gtgcatatgg agggatctgt
     4741 caatgggcat gagtttgaaa ttgaaggtga aggagaaggc cgaccatatg agggaacaca
     4801 aaccgcaaaa ctaaaggtaa gtttaaacat atatatacta actaaccctg attatttaaa
     4861 ttttcaggta actaaaggcg gaccattacc attcgcctgg gacatcctct ctccacagtt
     4921 catgtatgga agtaaagctt atgttaaaca tccggcagat ataccagatt atttgaaact
     4981 ttcattcccg gagggtttta agtgggaacg cgtaatgaat tttgaagacg gaggagttgt
     5041 tacagtgacg caagactcaa ggtaagttta aacagttcgg tactaactaa ccatacatat
     5101 ttaaattttc agcctccaag atggagaatt tatttataaa gtcaaacttc gaggaacgaa
     5161 tttcccctcg gatggatctg ttatgcagaa gaagactatg ggatgggaag cttcaagtga
     5221 aagaatgtac cctgaagacg gtgctcttaa gggagagatt aaacaacgtc ttaaattgaa
     5281 agatggagga cattacgatg ctgaggtaag tttaaacatg attttactaa ctaactaatc
     5341 tgatttaaat tttcaggtga agacaactta caaagccaaa aaaccagttc agctgccagg
     5401 agcgtacaat gttaatatta aactggatat cacctcccac aacgaggatt acactatcgt
     5461 tgagcaatat gaaagagctg aagggcggca ctcgacaggt ggcatggatg aattgtataa
     5521 gtaggagctc ccgagatctt cagcgctcag cattcgtaga attgtaagtt taaactttct
     5581 catactaact aacgcgctct atttaaattt tcagaattcc aactgagcgc cggtcgctac
     5641 cattaccaac ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa
     5701 actgagttct actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc
     5761 tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca
     5821 ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt
     5881 taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa
     5941 agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac
     6001 aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata
     6061 gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt
     6121 ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt
     6181 ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt
     6241 ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct
     6301 aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag
     6361 gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt
     6421 ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg
     6481 gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     6541 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     6601 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     6661 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     6721 aaaataccgc atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg
     6781 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6841 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6901 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6961 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7021 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7081 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7141 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7201 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7261 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7321 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7381 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7441 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7501 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7561 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7621 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7681 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7741 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7801 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7861 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7921 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7981 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8041 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8101 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8161 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8221 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8281 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8341 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8401 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8461 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8521 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8581 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8641 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8701 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8761 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8821 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     8881 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8941 acacaggaaa cagctatgac catgattacg ccaagctgta agtttaaaca tgatcttact
     9001 aact
//
