LOCUS       sequence-35547-e         629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL3
FEATURES             Location/Qualifiers
     source          1..629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             119..139
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             140..166
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt agatctaact
       61 cgagaaagat attgtatata tcgtaacaat aggaggttca acaatggctt cctcccctcc
      121 aaagaaaaag agaaaggtta gttggaagga cgcaagtggt tggtctagaa tgcatgcgga
      181 tcccattcgt ccgcgcaggc caagtcctgc ccgcgagctt ctgcccggac cccaaccgga
      241 tagggttcag ccgactgcag atcgtggggt gtctgcgcct gctggcagcc ctctggatgg
      301 cttgcccgct cggcggacgg tgtcccggac ccggctgcca tctccccctg cgccctcacc
      361 tgcgttctcg gcgggcagct tcagcgatct gctccgtccg ttcgatccgt cgcttcttga
      421 tacatcgctt cttgattcga tgcctgccgt cggcacgccg catacagcgg ctgccccagc
      481 agagtgggat gaggcgcaat cggctctgcg tgcagccgat gacccgccac ccaccgtgcg
      541 tgtcgctgtc actgccgcgc ggccgccgcg cgccaagccg gccccgcgac ggcgtgctgc
      601 gcaaccctcc gacgcttcgc cggccgcgc
//
