LOCUS       sequence-35548-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL3
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(22..27,28..45,46..52)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    60..76
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     65..87
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     84..100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     84..100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             96..503
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     119..139
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        121..139
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(186..205)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(187..205)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(215..232)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(215..231)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(224..246)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
       61 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      121 aattaaccct cactaaaggg aacaaaagct ggaggacctc gagggggggc ccggtaccca
      181 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
      241 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      301 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      361 atggcgaatg ggacgcgccc tgtagcggcg cccgtctcca ggggggcacc cgtcagtgca
      421 ttgcgcgatg catgcactgc ctccattgcg gtcacgccgc cacgttttgc aatcttcaca
      481 agttggcctg tgtccaactg taacggcgga cctctcaact cccccgcatc cgtgagcaag
      541 gcctccaggg cgcgtgcgcc ggaccactgt ttgccgacgc caacgatgtc ttcgtgtgtc
      601 gcctctggca acgccgtgat tatgtgctga tacgtgacag cgacggtccc taacgctgcc
      661 gggtgttggc tgagcgcaac gatgtgcgcg tgtgtaaacc catggcccac cagtgcctcg
      721 tggtgctgc
//
