LOCUS       sequence-35549-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL3
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..379)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCN"
     primer_bind     229..251
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     243..260
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     244..260
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     270..289
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        270..288
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(336..356)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(336..354)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(375..391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(375..391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(388..410)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    399..415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(423..429,430..447,448..453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    468..489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gattgcaaaa cgtggcggcg tgaccgcaat ggaggcagtg catgcatcgc gcaatgcact
       61 gacgggtgcc cccctggaga cgggcgccgc tacagggcgc gtcccattcg ccattcaggc
      121 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      181 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      241 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      301 ccgggccccc cctcgaggtc ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
      361 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      421 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      481 ctcacattaa ttgcgttgcg ctcactgccc gctttccacc ggtcgtctcc aacgaccacc
      541 tcgtcgcctt ggcctgcctc ggcggacgtc ctgccatgga tgcagtgaaa aagggattgc
      601 cgcacgcgcc ggaattgatc anaagagtca atcgccgtat tggcgaacgc acgtcccatc
      661 gcgttgccga ctacgcgcaa gtggttcgcg tgctggagtt tttccagtgc cactcccacc
      721 cagcgtacgc atttgatgag gccatgacgc agttcgggat gagcaggaac gggttggtac
      781 agctctttcg cagagtgggc gtcaccgaac tcgaagcccg cggtggaacg ctcc
//
