LOCUS       sequence-355539-        8821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-4::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural 
            expression of TWK-18-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH2108
FEATURES             Location/Qualifiers
     source          1..8821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(124..129,130..147,148..154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     167..189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     186..202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6592..6614)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6714..6733
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6811..6829)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6897..7001
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7002..7070,7071..7862)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7220..7239)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8033..8621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8522..8541
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8775..8792
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      181 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
      241 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      301 gatggatacg ctaacaactt ggaaatgaaa taagcttggg ctgcagcatg cggccgcttc
      361 cccaaattgg aacagtgaaa tactcaatca atcactctgg cggttttctc cacaaagaac
      421 acgaaattgg aaaagggaaa agattgattt gtttgaaggc gtgaggaaat cgaaaaaaaa
      481 atgtttcaaa acaaatgaaa tattttaatg aataaaccca ggaatgccac cttttggtgc
      541 ccgcctacaa aaaagtcaac tgttcttaaa ctcctacata gttgccctct ctaacgaatg
      601 ttgctttttt taaattttca ttgaatttga ttatcgtctg tatgcgccta ctctatcagt
      661 ggaaaacatg cccatttccc acaaatttac tcgtttggag ctcaaaatgt taaatggggc
      721 atcatggaat tggcatctca cagaaagtgg cggatgagtt atatgtaata ataatggtgg
      781 ctgaagtgga aaccgcaaaa aaaggatgtg tatttgagat tttgtaacgg aatttgaatc
      841 agatacacaa aatgagagaa atttatagat tctttaaaat ccacctgaag tcggcaaatt
      901 tttgaaaggt ctgttcgttc agtttttcat aagttttatg aattttgatt tactactgaa
      961 gttcgagaaa agccctttaa ccataccaat aagaaattat gttaacttta caagaatatt
     1021 aacaaaaaat tattaaaaaa ctgtgttccc ctggcattat gatatttcca ccgtcagttc
     1081 tctattaaaa cttcaactct acacagtttc aactctcaaa tgagctgtcc atcttttgcc
     1141 ttcaggaaat tatgctaatc tgctgatttc gcgcactttc accacactat ttccaaatag
     1201 aaggaggggt ggtgcaacac acgagacact gcaaaaaggt tcagtgaaga tttatctact
     1261 tgtgagatga gagcaggagg ggtgggagaa gtgtgatgat tgacgggatg acaagaacag
     1321 tgatgtggtg tcgaaatgga agatgcgcta atttgtgggg ttttatgaaa tttgaataat
     1381 tacatttata acatctacta gttaggcgtc tgtgtaggta ttgcctgcct gcctacaagg
     1441 catatgtacg aggcaggtta aacctcctag aaagctacgg tactttcaaa ccacataaca
     1501 tttatactgg tgtcttttcg cgtcttcttc ttgaacatgt agaccctcaa aaagctaagt
     1561 tttacactgt gtagtttgct ttgaaatcta caaaattgga ggcacgcctt ctggactttg
     1621 ccgacacgca tactttctac ctaccaactg cctacttcca cagtctagcg tgaactccaa
     1681 taccatttta aaaattgttt tatctcaaat tttcaatttt taaatccaat ttcccagttc
     1741 taaaaaaaaa attttttttt taatttaaaa actctcatcc tttttaatgc tcgttcccat
     1801 tcggcaagac gtgtaccaaa cacaaaacaa attgactggt gatccatctc gaatttctcg
     1861 tttcaaatta gtcgttgcta tatatattgt cttatgtgtg gaaaacgaat aagaaaaatt
     1921 agagaaagat gagagagcag gggcagaatg aaaatttgca tggaaattga agaagatcac
     1981 tgctttttcc gatgaatgat gactaaatag aatgagttga gaggaaaata atcataattc
     2041 taattttaat tagaaatatg atatggaaag taagcaaaac cacaaggctt ttgctctgaa
     2101 cttatttccg aaaaaaaaaa gtgaaactga aagttagaat tttcaaaata atattgcgag
     2161 agtcaacctt cagaaaaatt ggcgagaaat caaatttgaa ttctctgaaa atttagttaa
     2221 aatacatatg ttatccttct ttgttttttg gccttagcct gccttcaagg cgacctgagc
     2281 ctatgcgggt gcctaatacc gaaacacaca taattttttt ttttaatctt taaatactgt
     2341 aacgagtcgc gagatccagc gagacccata gaatatgttg agcgctgcaa acaatcgtat
     2401 aactcttgtt caaatttttc cactctgtat ttttgcaagc agttcaacaa atggtcatca
     2461 tttttataac ccatatctat attcgaatct gacataacaa ataatactaa gcacatcctc
     2521 ccacatctct tttcattcaa gttgcgcaat tcccctaata actatggatg gaggggggac
     2581 cgagcccaaa gattattcaa aacgataacg actttttcat gcggccaacg aaaaataaaa
     2641 cttttctcat attctcctat ttcccatttc aagaattaca tccccatggg acgttgaaat
     2701 cacatcatat tagtatatgt taatagttct tttcactgga atatcgccac gccccccttc
     2761 cggtggctca ttggtttcga gaagaaggcg gagccgaagt gggttcttct ttcttttttt
     2821 gtggtagaag tctagcagga ttaagctact attccattag aggatcttct tcttccaaaa
     2881 agtgaaaatc ccgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata
     2941 acatagaaca ttttcaggag gacccttggc tagcgtcgac ggatccaaat ggcgattgtt
     3001 gcgcaaggag tttctacgat tttgacgacg tttcagaaaa cgttcaaagg attactccca
     3061 ttgataattc ttgtggctta cactctattg ggagcatgga tcttctggat gatcgaagga
     3121 gaaaacgaac gagagatgct cattgagcaa caaaaagaaa gggatgaact catccggcga
     3181 actgtctata aaattaatca attgcaaatc aaaagacaga gaagattaat gacggccgag
     3241 gaagaataca acagaactgc aaaagtgttg actacctttc aagaaacttt gggaattgta
     3301 ccagctgata tggacaagga tattcattgg acattcttgg gatcaatctt ctattgcatg
     3361 acagtctata caactattgg ttacggaaac attgttcctg gaactggatg gggtcgtttt
     3421 gctactatcc tttacgcatt cattggaatt ccactcacag tgctcagtct ttactgtctt
     3481 ggtagtcttt ttgccaaggg atgcaaaatg ttgtggagat tttttttgaa gtccaccagg
     3541 gttgtgagca aagatttgtc aaataagatt tctgaagctg ccgataatat tgaagaagga
     3601 acaacggcaa ttactccatc agccgagaaa acagaaaata acgatgatga cttgttgtct
     3661 ttccccatct ccggcctctt gcttattacc gtcatctggg tcattttctg tgcggtcttg
     3721 ttcaccttcc tagaagaatg ggactttgga acatctctct acttcacatt gatttctttc
     3781 acaaccatcg gttttggaga cattcttcca tcggattacg atttcatacc aattgttgga
     3841 gttcttcttc tcattggtct ttctttggtc tctacagtga tgacactcat tcaacagcaa
     3901 attgaagcat tagcatcggg aatgaaagat aacattgacc aagaatatgc tcgtgcattg
     3961 aacgaggctc gtgaagacgg agaggttgac gagcacgtag atccagaaga agatccggag
     4021 aacaataaaa agtcctttga cgctgttatc tccagaatga actggtccaa gagagggttg
     4081 tattatcttc ttcctgatag ccagaagaaa gaactcgcaa agcagtctga gaagaaaatg
     4141 ggaagaaaaa gtattaaaat tcagactgat aacgatttgt tggaaacact cattcgggag
     4201 gaaattttga aggcggagct caacaatgag atgcacaaat atacagcacc acgatcttca
     4261 caccagccaa aacttgtcta ttctgatgtt cgcgagaagg aggttccaat tgaagttgtc
     4321 cgtgttgagc atttcaatca cggaaatgaa gactatctag agcatgacat ctagtggatc
     4381 cccgagatct tcagcgctca gcattcgtag aattgtaagt ttaaactttc tcatactaac
     4441 taacgcgctc tatttaaatt ttcagaattc gctgtctcat cctactttca cctagttaac
     4501 tgcttgtctt aaaatctatg cttctcttta gtatctaaaa ttttcctaga agcttacaag
     4561 tatataaatg gtctcttctc aataaaggtt gtatatttat tcatcttatt gaatctgcca
     4621 tttcctcgtt tttgcgagtt tatatacctt ccaattttct ttctattgta ttttcaactt
     4681 ctaattttaa ttcagggaaa ctgcttcaac gcatcggtac cctatggtct caaagggtga
     4741 agaagataac atggcaatta ttaaagagtt tatgcgtttc aaggtgcata tggagggatc
     4801 tgtcaatggg catgagtttg aaattgaagg tgaaggagaa ggccgaccat atgagggaac
     4861 acaaaccgca aaactaaagg taagtttaaa catatatata ctaactaacc ctgattattt
     4921 aaattttcag gtaactaaag gcggaccatt accattcgcc tgggacatcc tctctccaca
     4981 gttcatgtat ggaagtaaag cttatgttaa acatccggca gatataccag attatttgaa
     5041 actttcattc ccggagggtt ttaagtggga acgcgtaatg aattttgaag acggaggagt
     5101 tgttacagtg acgcaagact caaggtaagt ttaaacagtt cggtactaac taaccataca
     5161 tatttaaatt ttcagcctcc aagatggaga atttatttat aaagtcaaac ttcgaggaac
     5221 gaatttcccc tcggatggat ctgttatgca gaagaagact atgggatggg aagcttcaag
     5281 tgaaagaatg taccctgaag acggtgctct taagggagag attaaacaac gtcttaaatt
     5341 gaaagatgga ggacattacg atgctgaggt aagtttaaac atgattttac taactaacta
     5401 atctgattta aattttcagg tgaagacaac ttacaaagcc aaaaaaccag ttcagctgcc
     5461 aggagcgtac aatgttaata ttaaactgga tatcacctcc cacaacgagg attacactat
     5521 cgttgagcaa tatgaaagag ctgaagggcg gcactcgaca ggtggcatgg atgaattgta
     5581 taagtaggag ctcccgagat cttcagcgct cagcattcgt agaattgtaa gtttaaactt
     5641 tctcatacta actaacgcgc tctatttaaa ttttcagaat tccaactgag cgccggtcgc
     5701 taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt
     5761 taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc gcgcccgtgc
     5821 ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc
     5881 ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc
     5941 ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
     6001 aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
     6061 cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc
     6121 atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa
     6181 tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa
     6241 gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg
     6301 cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt
     6361 gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt
     6421 gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg
     6481 tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt ttcctactag
     6541 tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     6601 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     6661 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     6721 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     6781 gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt ttataggtta
     6841 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6901 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6961 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7021 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7081 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7141 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7201 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7261 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7321 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7381 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7441 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7501 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7561 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7621 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7681 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7741 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7801 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7861 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7921 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7981 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8041 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8101 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8161 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8221 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8281 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8341 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8401 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8461 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8521 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8581 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8641 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8701 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8761 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8821 c
//
