LOCUS       sequence-355541-        7986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-4::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A-MN and 
            other neurals expression of miniSOG-SL2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 7986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH2843
FEATURES             Location/Qualifiers
     source          1..7986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..22
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11..33
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     30..46
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     30..46
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3064..3384
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(5601..5623)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5723..5742
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5820..5838)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5906..6010
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6011..6079,6080..6871)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6229..6248)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7042..7630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7531..7550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7784..7801
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7918..7939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7954..7959,7960..7977,7978..7984)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
       61 gctgtaagtt taaacatgat cttactaact aactattctc atttaaattt tcagagctta
      121 aaaatggctg aaatcactca caacgatgga tacgctaaca acttggaaat gaaataagct
      181 tgggctgcag catgcggccg cttccccaaa ttggaacagt gaaatactca atcaatcact
      241 ctggcggttt tctccacaaa gaacacgaaa ttggaaaagg gaaaagattg atttgtttga
      301 aggcgtgagg aaatcgaaaa aaaaatgttt caaaacaaat gaaatatttt aatgaataaa
      361 cccaggaatg ccaccttttg gtgcccgcct acaaaaaagt caactgttct taaactccta
      421 catagttgcc ctctctaacg aatgttgctt tttttaaatt ttcattgaat ttgattatcg
      481 tctgtatgcg cctactctat cagtggaaaa catgcccatt tcccacaaat ttactcgttt
      541 ggagctcaaa atgttaaatg gggcatcatg gaattggcat ctcacagaaa gtggcggatg
      601 agttatatgt aataataatg gtggctgaag tggaaaccgc aaaaaaagga tgtgtatttg
      661 agattttgta acggaatttg aatcagatac acaaaatgag agaaatttat agattcttta
      721 aaatccacct gaagtcggca aatttttgaa aggtctgttc gttcagtttt tcataagttt
      781 tatgaatttt gatttactac tgaagttcga gaaaagccct ttaaccatac caataagaaa
      841 ttatgttaac tttacaagaa tattaacaaa aaattattaa aaaactgtgt tcccctggca
      901 ttatgatatt tccaccgtca gttctctatt aaaacttcaa ctctacacag tttcaactct
      961 caaatgagct gtccatcttt tgccttcagg aaattatgct aatctgctga tttcgcgcac
     1021 tttcaccaca ctatttccaa atagaaggag gggtggtgca acacacgaga cactgcaaaa
     1081 aggttcagtg aagatttatc tacttgtgag atgagagcag gaggggtggg agaagtgtga
     1141 tgattgacgg gatgacaaga acagtgatgt ggtgtcgaaa tggaagatgc gctaatttgt
     1201 ggggttttat gaaatttgaa taattacatt tataacatct actagttagg cgtctgtgta
     1261 ggtattgcct gcctgcctac aaggcatatg tacgaggcag gttaaacctc ctagaaagct
     1321 acggtacttt caaaccacat aacatttata ctggtgtctt ttcgcgtctt cttcttgaac
     1381 atgtagaccc tcaaaaagct aagttttaca ctgtgtagtt tgctttgaaa tctacaaaat
     1441 tggaggcacg ccttctggac tttgccgaca cgcatacttt ctacctacca actgcctact
     1501 tccacagtct agcgtgaact ccaataccat tttaaaaatt gttttatctc aaattttcaa
     1561 tttttaaatc caatttccca gttctaaaaa aaaaattttt tttttaattt aaaaactctc
     1621 atccttttta atgctcgttc ccattcggca agacgtgtac caaacacaaa acaaattgac
     1681 tggtgatcca tctcgaattt ctcgtttcaa attagtcgtt gctatatata ttgtcttatg
     1741 tgtggaaaac gaataagaaa aattagagaa agatgagaga gcaggggcag aatgaaaatt
     1801 tgcatggaaa ttgaagaaga tcactgcttt ttccgatgaa tgatgactaa atagaatgag
     1861 ttgagaggaa aataatcata attctaattt taattagaaa tatgatatgg aaagtaagca
     1921 aaaccacaag gcttttgctc tgaacttatt tccgaaaaaa aaaagtgaaa ctgaaagtta
     1981 gaattttcaa aataatattg cgagagtcaa ccttcagaaa aattggcgag aaatcaaatt
     2041 tgaattctct gaaaatttag ttaaaataca tatgttatcc ttctttgttt tttggcctta
     2101 gcctgccttc aaggcgacct gagcctatgc gggtgcctaa taccgaaaca cacataattt
     2161 ttttttttaa tctttaaata ctgtaacgag tcgcgagatc cagcgagacc catagaatat
     2221 gttgagcgct gcaaacaatc gtataactct tgttcaaatt tttccactct gtatttttgc
     2281 aagcagttca acaaatggtc atcattttta taacccatat ctatattcga atctgacata
     2341 acaaataata ctaagcacat cctcccacat ctcttttcat tcaagttgcg caattcccct
     2401 aataactatg gatggagggg ggaccgagcc caaagattat tcaaaacgat aacgactttt
     2461 tcatgcggcc aacgaaaaat aaaacttttc tcatattctc ctatttccca tttcaagaat
     2521 tacatcccca tgggacgttg aaatcacatc atattagtat atgttaatag ttcttttcac
     2581 tggaatatcg ccacgccccc cttccggtgg ctcattggtt tcgagaagaa ggcggagccg
     2641 aagtgggttc ttctttcttt ttttgtggta gaagtctagc aggattaagc tactattcca
     2701 ttagaggatc ttcttcttcc aaaaagtgaa aatcccggga ttggccaaag gacccaaagg
     2761 tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct tggctagcgt
     2821 cgacggatcc cgatgtcgga cacaattctt ggtttcaaca aatcaaacgt cgttttggct
     2881 gctggaattg ctggagccgc tttcctcggc tactgcattt acttcgatca taagagaatc
     2941 aacgctccag actacaagga caagattagg caaagtcagt gttttaacaa catatttcct
     3001 tcggattttt atctaaaaac aacttatttt ctttcagaga gacgtgccca ggctggggat
     3061 cctatggaga aaagtttcgt gataactgat ccacggctgc cagacaatcc catcatcttc
     3121 gcatccgatg gcttcctgga gctgaccgag tattccagag aggagatcct gggccgcaat
     3181 ggccgctttc tgcagggacc agagacagac caggccacag tgcagaagat tcgcgatgcc
     3241 attagagatc agcgcgagat taccgtgcag ctgataaact acacaaaaag cgggaagaaa
     3301 ttctggaacc tcctgcacct ccagcccatg agggaccaga agggtgagct ccagtatttc
     3361 atcggagtgc agctggatgg ataaagatcc ccgagatctt cagcgctcag cattcgtaga
     3421 attgtaagtt taaactttct catactaact aacgcgctct atttaaattt tcagaattcg
     3481 ctgtctcatc ctactttcac ctagttaact gcttgtctta aaatctatgc ttctctttag
     3541 tatctaaaat tttcctagaa gcttacaagt atataaatgg tctcttctca ataaaggttg
     3601 tatatttatt catcttattg aatctgccat ttcctcgttt ttgcgagttt atataccttc
     3661 caattttctt tctattgtat tttcaacttc taattttaat tcagggaaac tgcttcaacg
     3721 catcggtacc ctatggtctc aaagggtgaa gaagataaca tggcaattat taaagagttt
     3781 atgcgtttca aggtgcatat ggagggatct gtcaatgggc atgagtttga aattgaaggt
     3841 gaaggagaag gccgaccata tgagggaaca caaaccgcaa aactaaaggt aagtttaaac
     3901 atatatatac taactaaccc tgattattta aattttcagg taactaaagg cggaccatta
     3961 ccattcgcct gggacatcct ctctccacag ttcatgtatg gaagtaaagc ttatgttaaa
     4021 catccggcag atataccaga ttatttgaaa ctttcattcc cggagggttt taagtgggaa
     4081 cgcgtaatga attttgaaga cggaggagtt gttacagtga cgcaagactc aaggtaagtt
     4141 taaacagttc ggtactaact aaccatacat atttaaattt tcagcctcca agatggagaa
     4201 tttatttata aagtcaaact tcgaggaacg aatttcccct cggatggatc tgttatgcag
     4261 aagaagacta tgggatggga agcttcaagt gaaagaatgt accctgaaga cggtgctctt
     4321 aagggagaga ttaaacaacg tcttaaattg aaagatggag gacattacga tgctgaggta
     4381 agtttaaaca tgattttact aactaactaa tctgatttaa attttcaggt gaagacaact
     4441 tacaaagcca aaaaaccagt tcagctgcca ggagcgtaca atgttaatat taaactggat
     4501 atcacctccc acaacgagga ttacactatc gttgagcaat atgaaagagc tgaagggcgg
     4561 cactcgacag gtggcatgga tgaattgtat aagtaggagc tcccgagatc ttcagcgctc
     4621 agcattcgta gaattgtaag tttaaacttt ctcatactaa ctaacgcgct ctatttaaat
     4681 tttcagaatt ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat
     4741 aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata
     4801 tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac
     4861 atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac
     4921 ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt
     4981 agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc
     5041 cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt
     5101 ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc
     5161 atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca
     5221 tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca
     5281 agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt
     5341 gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg
     5401 caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat
     5461 aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca
     5521 atataccaaa cataactgtt tcctactagt cggccgtacg ggccctttcg tctcgcgcgt
     5581 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     5641 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     5701 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     5761 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg
     5821 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     5881 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5941 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6001 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6061 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6121 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6181 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6241 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6301 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6361 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6421 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6481 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6541 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6601 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6661 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6721 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6781 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6841 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6901 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6961 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7021 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7081 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7141 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7201 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7261 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7321 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7381 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7441 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7501 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7561 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7621 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7681 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7741 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7801 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7861 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     7921 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     7981 gttgtg
//
