LOCUS       sequence-355563-        6057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for Opto-cytTrkB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Hong J , Heo WD
  TITLE     Optogenetic Modulation of TrkB Signaling in the Mouse Brain.
  JOURNAL   J Mol Biol. 2020 Feb 14;432(4):815-827. doi: 
            10.1016/j.jmb.2020.01.010. Epub 2020 Jan 18.
  PUBMED    31962123
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2020.01.010"; journalName: "J Mol Biol"; date: 
            "2020-02-14- 14"; volume: "432"; issue: "4"; pages: "815-827"
COMMENT     Sequence Label: pAAV-Camk2a(0.4)-Lyn-PHR(E281A)-HA
FEATURES             Location/Qualifiers
     source          1..6057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      533..542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2132..2158
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2186..2774
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2239..2259)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2657..2668)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2777..2793)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2778..2794)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2804..3280
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3003..3022)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3320..3460
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3320..3449
                     /label=AAV2 ITR
     rep_origin      3535..3990
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3622..3641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3832..3853
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4007..4026)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4126..4148
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4186..4204)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4272..4376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4377..4445,4446..5237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4595..4614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5408..5996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5897..5916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacttg tggactaagt ttgttcacat ccccttctcc
      181 aaccccctca gtacatcacc ctgggggaac agggtccact tgctcctggg cccacacagt
      241 cctgcagtat tgtgtatata aggccagggc aaagaggagc aggttttaaa gtgaaaggca
      301 ggcaggtgtt ggggaggcag ttaccggggc aacgggaaca gggcgtttcg gaggtggttg
      361 ccatggggac ctggatgctg acgaaggctc gcgaggctgt gagcagccac agtgccctgc
      421 tcagaagccc caagctcgtc agtcaagccg gttctccgtt tgcactcagg agcacgggca
      481 ggcgagtggc ccctagttct gggggcagct ctagagccga tccccgggta ccgccaccat
      541 gggatgtata aaatcaaaag ggaaagacag cgcgggagca gatagtgctg gtagtgctgg
      601 tagtgctggg aattccatga agatggacaa aaagaccatc gtctggtttc ggagagattt
      661 gagaatagaa gataatcccg cgctcgccgc cgcggcccac gagggttccg tcttccccgt
      721 tttcatttgg tgtcctgaag aagaaggcca gttttatccc ggaagggcct ctaggtggtg
      781 gatgaagcaa agtctggccc atcttagcca gtcactgaaa gcactgggca gtgatcttac
      841 cctgatcaag acacacaata ccatctctgc catcctcgac tgcatcaggg tgaccggcgc
      901 aacgaaagtc gtgtttaacc acctgtacga tccagttagt ctggtgcgcg accacactgt
      961 gaaggagaag ctggtggaac gggggatcag tgtgcagagc tacaacgggg accttctgta
     1021 cgagccatgg gagatctatt gcgagaaagg gaaaccgttc acctccttca acagttactg
     1081 gaagaaatgt ttggatatgt caatagagtc cgttatgttg ccccctccct ggagactgat
     1141 gccgattact gctgctgcag aggccatctg ggcctgctcc atcgaggaac tcggtctgga
     1201 aaatgaagca gaaaagccaa gcaatgcact tctcactaga gcctggagcc ccggctggtc
     1261 taatgccgac aagctgctta acgagttcat cgaaaaacaa ctgattgact acgcgaagaa
     1321 ctccaagaaa gtggtaggta actcaactag cttgctctct ccatatctcc attttggcga
     1381 gatttctgtc cgccatgtat ttcagtgcgc tcggatgaaa cagattatct gggctcgcga
     1441 taaaaacagc gaaggcgccg aaagcgccga tctgttcctg cgagggatcg gacttcggga
     1501 atactcccgg tatatatgtt tcaactttcc attcacacac gagcagagtc tgttgtccca
     1561 cctcaggttc ttcccctggg acgccgatgt cgacaaattc aaggcatgga gacagggaag
     1621 gacagggtac ccactcgtgg atgctggcat gagagagctc tgggctacag gctggatgca
     1681 caaccgcatc cgggtaatcg tgtcctcatt tgctgtcaag tttctgctcc tgccttggaa
     1741 atggggaatg aagtactttt gggataccct tctcgacgcc gacttggagt gtgacattct
     1801 gggatggcaa tatattagcg ggtcaattcc tgacggccat gagttggaca ggttggacaa
     1861 tccggccttg cagggagcta agtatgatcc cgaaggagag tatattcgac agtggctccc
     1921 cgagctggcc cgacttccta cggagtggat tcaccatcct tgggacgcac cactgacagt
     1981 gctcaaggca agcggggtgg agctgggcac caattacgct aagcctatag ttgatataga
     2041 tacagcacgc gagctgctgg ctaaagcgat ctctcgcact cgggaggcgc agattatgat
     2101 cggtgctgcc ggcagcggca gcccaccggt atatccatat gatgttccag attatgctta
     2161 agaattggat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     2221 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2281 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2341 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2401 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2461 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2521 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2581 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     2641 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     2701 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     2761 ggccgcctcc ccgcatcgat accgtcgacc tcgagagatc tacgggtggc atccctgtga
     2821 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
     2881 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
     2941 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
     3001 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
     3061 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
     3121 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
     3181 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
     3241 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac
     3301 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     3361 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     3421 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     3481 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     3541 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3601 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3661 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3721 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     3781 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3841 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     3901 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3961 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     4021 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4081 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4141 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     4201 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4261 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4921 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5281 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5461 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5521 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5581 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5641 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5701 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5761 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5821 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5881 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5941 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6001 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
