LOCUS       sequence-355656-        4089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ddRFP sensor of Hras.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Kim J , Lee S, Jung K, Oh WC, Kim N, Son S, Jo Y, Kwon HB,  Heo WD
  TITLE     Intensiometric biosensors visualize the activity of multiple small 
            GTPases in vivo.
  JOURNAL   Nat Commun. 2019 Jan 14;10(1):211. doi: 10.1038/s41467-018-08217-3.
  PUBMED    30643148
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-14"; volume: "10(1):211. doi"; pages: " 
            10.1038/s41467-018-08217-3"
COMMENT     Sequence Label: pAAV-CAG-R-Hras
FEATURES             Location/Qualifiers
     source          1..4089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     366..388
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     450..469
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1520..1576
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2321..2890
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=H-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     misc_feature    2899..3487
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2952..2972)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3370..3381)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3530..3651
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3541..3560)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3595..3614
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3813..3942
                     /label=AAV2 ITR
ORIGIN
        1 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
       61 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
      121 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
      181 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
      241 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
      301 ggggggagcg gctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
      361 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
      421 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
      481 ttttggcaaa gaattgctcg aggtcaccat ggccaccatc aaagagttca tgcgcttcaa
      541 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      601 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
      661 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccgaggcgt acgtgaggca
      721 ccccgccgac atccccgatt acaagaagct gcccttcccc gagggcttca agtgggagcg
      781 cgtgatgaac ttcgaagacg gcggtctggt gaccgttacc caggactcct ccctgcagga
      841 cggcacgctg atctgcaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
      901 aatgcagaag aagaccatgg gctgggaggc ctccaccgag atgctgtacc ccgaagacgg
      961 cgtgctgaag ggccatagct atcaggccct gaagctgaag gacggcggcc actacctggt
     1021 ggagttcgag accatctaca tggccaagaa gcccgtgcaa ctgcccggcg attactgtgt
     1081 ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacgg
     1141 gcgctccgag ggccgccacc gtctgggcat ggacgagcgg tacaagttag ggcatggtac
     1201 aggtagcaca ggcagcggta gctccggtac cctgtacaag tccggactca gatctcgagc
     1261 caccatgcct tctaagacaa gcaacactat ccgtgttttc ttgccgaaca agcaaagaac
     1321 agtggtcaat gtgcgaaatg gaatgagctt gcatgactgc cttatgaaag cactcaaggt
     1381 gaggggcctg caaccagagt gctgtgcagt gttcagactt ctccacgaac acaaaggtaa
     1441 aaaagcacgc ttagattgga atactgatgc tgcgtctttg attggagaag aacttcaagt
     1501 agatttcctg ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     1561 agagaatcct ggaccgatgg tgagcaagag cgaggaggtc atcaaagagt tcatgcgctt
     1621 caaggtgcgc ttggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
     1681 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     1741 gcccttcgcc tgggacatcc tgtcccccca gatcatgtac ggctccaagg cgtacgtgaa
     1801 gcaccccgcc gacgtccccg attacatgaa gctgtccttc cccgagggct tcaagtggga
     1861 gcgcgtgatg cacttcgagg acggcggtct ggtgaccgtg actcaggaca cctccctgca
     1921 ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc
     1981 cgtaatgcag aagaagacct tgggctggga ttatgccacc gagcgcctgt accccgaaga
     2041 cggcgtgctg aagggcgagc ttctggggcg cctgaagctg aaggacggcg gcctcaacct
     2101 ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg gctactactt
     2161 cgtggacacc aagctgggca tcacctccca caacgaggac tacaccatcg tggaacagta
     2221 cgagcgctcc gagggccgcc accacctggg gcatggcacc ggcagcaccg gcagcggcag
     2281 ctccggtacc gcctcctccg aggacaacaa cctgtacaag atgacggaat ataagctggt
     2341 ggtggtgggc gccggcggtg tgggcaagag tgcgctgacc atccagctga tccagaacca
     2401 ttttgtggac gaatacgacc ccactataga ggattcctac cggaagcagg tggtcattga
     2461 tggggagacg tgcctgttgg acatcctgga taccgccggc caggaggagt acagcgccat
     2521 gcgggaccag tacatgcgca ccggggaggg cttcctgtgt gtgtttgcca tcaacaacac
     2581 caagtctttt gaggacatcc accagtacag ggagcagatc aaacgggtga aggactcgga
     2641 tgacgtgccc atggtgctgg tggggaacaa gtgtgacctg gctgcacgca ctgtggaatc
     2701 tcggcaggct caggacctcg cccgaagcta cggcatcccc tacatcgaga cctcggccaa
     2761 gacccggcag ggagtggagg atgccttcta cacgttggtg cgtgagatcc ggcagcacaa
     2821 gctgcggaag ctgaaccctc ctgatgagag tggccccggc tgcatgagct gcaagtgtgt
     2881 gctctcctga atctccggaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2941 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3001 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3061 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3121 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3181 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3241 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3301 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3361 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3421 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3481 tccccgcatc gataccgtcg acccgggcgg ccgcttcgag cagacatgat aagatacatt
     3541 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3601 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3661 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag
     3721 taaaacctct acaaatgtgg taaaatcgat aaggatcttc ctagagcatg gctacgtaga
     3781 taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac
     3841 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     3901 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcatta atgaatcggc
     3961 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4021 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4081 cggttatcc
//
