LOCUS       sequence-355676-        8462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of CeALG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Kuzuoglu-Ozturk D, Huntzinger E, Schmidt S, Izaurralde E
  TITLE     The Caenorhabditis elegans GW182 protein AIN-1 interacts with PAB-1 
            and subunits of the PAN2-PAN3 and CCR4-NOT deadenylase complexes.
  JOURNAL   Nucleic Acids Res. 2012 Jul;40(12):5651-65. doi: 10.1093/nar/gks218.
            Epub 2012 Mar 7.
  PUBMED    22402495
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks218"; journalName: "Nucleic Acids Res"; date: 
            "2012-07"; volume: "40"; issue: "12"; pages: "5651-65"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-CeALG1_O
FEATURES             Location/Qualifiers
     source          1..8462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5768..5809
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5819..5836
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5859..5876)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5909..6043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5933..5952)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5987..6006
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6644..7232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6724..6743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7403..8194,8195..8263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8026..8045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8264..8368)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8436..8454
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttgaattctg cagatatcca gcacagtggc ggccgcatgt ccggcgggcc
     2761 gcaatatttg ccaggagtca tgaattctac cattcaacag caaccacaga gcgcaacatc
     2821 gtcgtttctg ccaagtggac cgattagttc gacgtccacc tcctcgcaag ttgttccaac
     2881 atctggagcc acccagcagc cgcctttccc gtcggcacaa gcggctgcgt caacagcgct
     2941 ccaaaatgat ctagaagaaa tatttaattc tccgcctact cagccacaaa catttagcga
     3001 tgttcctcaa agacaagccg gaagcctggc tccaggagtt cccattggta atacaagtgt
     3061 ctcaattgga gaaccagcaa atacacttgg aggcggttta ccaggcggag caccgggaca
     3121 attacctggc ggcaaccaga gtggtattca attccaatgt cctcgacgcc caaaccacgg
     3181 agttgagggt cgctcaattc ttcttcgcgc caaccacttt gcagtgagaa ttcctggagg
     3241 aactattcaa cactaccagg tcgatgttac tccagacaag tgcccaagaa gagttaatcg
     3301 cgagattatc agctgcttga taagcgcatt ctccaagtac ttcactaata ttcgccctgt
     3361 ttacgatgga aagcgcaata tgtacacccg cgagcccctt ccaattggaa gagaacgtat
     3421 ggatttcgat gtcacacttc ctggagactc cgccgttgag cgtcaatttt cggtttcttt
     3481 gaaatgggtc ggacaagttt ctctctccac tctggaggat gccatggaag gaagagttcg
     3541 ccaggttcca ttcgaagccg ttcaagctat ggatgtcatt cttcgtcatc ttccaagctt
     3601 gaaatacact cctgtcggac gttcattctt ctcgccacca gttcctaatg cttctggagt
     3661 catggcagga tcatgccctc cccaggcttc tggagctgtc gcaggaggtg cccactccgc
     3721 tggacaatat cacgccgaga gcaaactcgg gggtggccgt gaagtctggt ttggattcca
     3781 tcagtcggtt cgcccatctc agtggaaaat gatgcttaac attgatgtct ctgcgactgc
     3841 tttctatcgt tccatgccag tcattgagtt catcgccgaa gttttggagc ttccagttca
     3901 agctcttgct gagcgtcgtg ctctatctga tgctcaacgt gtcaagttca ccaaggaaat
     3961 tcgtggtctc aaaattgaaa tcacccactg tggacaaatg cgcagaaagt atcgtgtctg
     4021 caacgtcact cgtcgtccag ctcaaactca aactttccca cttcaattgg agactggtca
     4081 aactattgag tgcactgtcg ccaagtattt ctatgacaag tacagaattc aactcaaata
     4141 cccacacttg ccatgcctcc aagttggaca ggaacaaaag cacacctacc tgccaccaga
     4201 ggtgtgcaac attgttccag gacaacgttg catcaagaag cttacggatg tgcagacatc
     4261 gacaatgatt aaggctacag ctcgttctgc tccagaacgt gagcgtgaga tttccaactt
     4321 ggtccgcaag gccgagttct cagctgatcc atttgctcac gaattcggaa tcaccatcaa
     4381 cccagcaatg actgaagtca agggacgcgt tctttctgct ccaaaacttc tctacggtgg
     4441 acgcactcga gccaccgctc tcccgaatca aggagtttgg gatatgcgtg gaaaacagtt
     4501 ccacactgga atcgatgttc gtgtctgggc gatcgcttgt tttgctcaac aacagcatgt
     4561 caaggagaac gatctccgca tgttcaccaa ccagcttcag cggatttcca acgacgctgg
     4621 tatgccaatc gtcggaaatc catgcttctg caagtacgct gttggagtcg agcaggttga
     4681 gccaatgttc aagtacttga aacaaaacta ctctggaatt caacttgtag tggttgttct
     4741 ccctggaaaa actccagttt atgctgaagt taaacgtgtt ggagacacag ttttgggtat
     4801 cgccacccag tgcgtgcaag ccaagaacgc aatccgaaca actccacaaa ctctttctaa
     4861 tttatgcttg aagatgaacg taaagcttgg aggagtcaac agtattctgc ttccaaacgt
     4921 acgcccaaga atcttcaacg aaccagtcat tttctttgga tgtgacatca ctcatccacc
     4981 agcaggagac tctcgcaaac catccatcgc agcagttgtc ggctcaatgg atgctcatcc
     5041 tagcagatat gctgcaaccg tccgtgtcca acaacatcgt caagagatca tctcagattt
     5101 aacctacatg gtccgcgagc ttcttgttca attctaccgc aacactcgct tcaagccagc
     5161 cagaatcgtt gtctaccgtg atggagtttc tgaggggcaa ttcttcaacg ttcttcaata
     5221 tgagcttcgc gcgattcgcg aggcttgcat gatgcttgaa agaggatatc aaccaggaat
     5281 cactttcatc gccgtccaaa agagacatca cactcgtctc tttgctgttg ataagaagga
     5341 tcaagtcgga aaagcataca atattccgcc aggaactact gtcgatgtag gaattaccca
     5401 cccaaccgag tttgacttct acttgtgctc tcatgctggt attcaaggaa catctcgtcc
     5461 atcccattac catgttcttt gggatgacaa caatctgaca gccgacgaac ttcaacagct
     5521 cacatatcaa atgtgccata cctacgtcag atgcacacga tccgtttcaa ttccagcgcc
     5581 agcatattat gctcatttgg tagcgttccg cgctcgttat catcttgtcg atcgcgagca
     5641 tgactctgga gaaggttctc agccatctgg aacttccgag gataccacct tgtcgaacat
     5701 ggctagagct gtgcaggttc atccggatgc caacaacgtc atgtactttg cttaaccgcg
     5761 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
     5821 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc
     5881 ctgagctcgc tgatcagcct cgatcgagga tccagacatg ataagataca ttgatgagtt
     5941 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     6001 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     6061 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     6121 ctacaaatgt ggtatggctg attatgatca gtcgaccgat gcccttgaga gccttcaacc
     6181 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     6241 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     6301 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     6361 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     6421 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     6481 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     6541 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggcc
     6601 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6661 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6721 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6781 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6841 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6901 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6961 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7021 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7081 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7141 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7201 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7261 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7321 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7381 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7441 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7501 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7561 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7621 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7681 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     7741 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7801 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7861 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7921 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7981 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     8041 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8101 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8161 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8221 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8281 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8341 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8401 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8461 gt
//
