LOCUS       sequence-355809-       10614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of NgCAM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Nabb AT, Bentley M
  TITLE     NgCAM and VAMP2 Reveal that Direct Delivery and Dendritic 
            Degradation Maintain Axonal Polarity.
  JOURNAL   Mol Biol Cell. 2021 Nov 3:mbcE21080425. doi: 
            10.1091/mbc.E21-08-0425.
  PUBMED    34731031
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2021 Nov 3:mbcE21080425. doi: 10.1091/mbc.E21-08-0425."
COMMENT     Sequence Label: pBa.NgCAM-mCherry
FEATURES             Location/Qualifiers
     source          1..10614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             5196..5900
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5338..5356)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5606..5625
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6033..6167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6070..6089)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6124..6143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6198..6214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6198..6214)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6211..6233)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6222..6238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6246..6252,6253..6270,6271..6276))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6291..6312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6429..6446)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6600..7188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6680..6699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7359..8150,8151..8219))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7982..8001
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8220..8324)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8392..8410
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8448..8470)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8570..8589
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8783..8805
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8797..8814
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8798..8814
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 cgcgcgccag gcggggcggg gsggggcgrg gggsggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
      901 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagctt ccgccatggc tctgccccat gggctcggcc
     1321 tcctcctgct cctgttgctg ggggggcccg gagccgccat caccattccc ccggagtatg
     1381 gtgcacacga tttcctgcag ccccccgagc tgacggagga acccccggaa caactcgtgg
     1441 tcttccccag tgatgacatc gtcctcaaat gcgtggccac cgggaacccc cccgtccagt
     1501 accgatggag ccgtgaggat cagcccttcg tccccgagga gcacgggggg gtctcggtgg
     1561 tccccggatc ggggactttg gtcatcaacg ccacgttggc cgcgcggctc caggggcgct
     1621 tccgctgctt cgccaccaac gcgttgggca ccgctgtgtc tcccgaggcc aacgtcatcg
     1681 ccgagaacac tccgcagtgg ccgaaggaga aggtgacccc ggtggaggtg gaggaggggg
     1741 accccgtggt gctgccctgt gacccccccg agagcgctgt gccccctaaa atctattggc
     1801 tcaacagcga catcgttcac atcgctcagg acgagagggt atcgatgggg caggatggga
     1861 acctctactt ctccaacgcc atggtgggcg acagccaccc cgactacatc tgccacgctc
     1921 acttcctcgg cccccgcacc atcatccaga aggagcccct cgacctccgc gtggccccca
     1981 gtaatgcggt tcggtcccgc cgcccccgcc tgctgctgcc ccgcgacccc caaacgacca
     2041 ccatcgccct ccgggggggc agcgtcgtgt tggagtgcat cgctgagggg ctccccactc
     2101 catgggtccg atggcggcgg ctgaatggcc ccctccccac ccgggcggcg ttggagaact
     2161 tcaacaaaac gctgcggctg tggggggtga cggagagcga cgacggggag tacgaatgtg
     2221 tggctgagaa cgggaggggg acggccaggg gcacccacag cgtcaccgtg gaggccgccc
     2281 catattgggt gcggcggcca cagagtgggg tcttcgggcc gggggagacg gcgaggctgg
     2341 actgcgaggt gggggggaaa ccccgacccc aaatccaatg gagcatcaat ggggtcccca
     2401 tcgacgcgct gccgggcgcg gagcggcggt ggctgcgggg cggcgctttg gtgcttccgg
     2461 agctgcggcc gaacgacagc gcggtgctgc agtgcgaggc gaggaaccgc cacggccgcc
     2521 tattggccaa cgccttcctg cacgtcgtgg agctgccggt ccgaatgctg acggcggatg
     2581 agcagcgcta cgaagtggtg gaaaaccaaa cggtgtttct gcactgcaga accttcgggg
     2641 cccccgcgcc aaacgtcgag tggctgaccc ccactttgga gccggctctg caggacgatc
     2701 gatccttcgt gttcaccaat gggagccttc ggctgagtgc ggtgcggggg ggggacgggg
     2761 gggtctacac ctgcatggcc caaaacgccc acagcaacgg cagcctcacg gcgctcctgg
     2821 aggtcagagc ccccacccga atttcggccc ccccccgaag cgccaccgcc aaaaaagggg
     2881 agacggtgac ctttcactgc ggggcgacct ttgaccccgc cgtgaccccc ggggagctgc
     2941 gatggctgcg gggggggcag ccgctgcccg acgacccccg gtattcggtg gcggcggaga
     3001 cgctgacggt gtccaacgtg gactatgggg acgaggggac cattcagtgc cgcgcctcca
     3061 cccctctcga ctccgcggag gccgaagcgc agctcagagt cgtgggccgc cccggcccca
     3121 tccgggacct ccaagtgatg gaggtggacg aacaccgcgt gcgcctcagc tggaccccgg
     3181 gggacgacca taacagcccc atagagaagt tcgtggtgga ggaggaggag ggcatcttca
     3241 gcgcggggcg cttcgcggag cggctgacgg ttccgggggg gcagccgtgg acgccccccc
     3301 tcccgctgtc cccatacggg cggttccgct tccgggtggt ggccgttaac gcctacggga
     3361 ggggggagca cagcgccccc agcgccccca tcgagacccc ccccgcggct ccggagcgca
     3421 acccgggggg ggtccatggg gagggcaatg agaccggcaa cctcgtcatc acctgggagc
     3481 ccctcccccc ccaggcctgg aacgccccct gggttcggta ccgcgtgcag tggcggccat
     3541 tggaggagcc cggcgggggg ggcccttcgg gggggttccc gtgggccgaa agcaccgtgg
     3601 acgccccccc cgtggtggtg ggggggctcc ccccgttcag ccccttccag atccgcgtcc
     3661 aggccgtgaa cggagccggg aagggaccgg aagcgacccc cggcgtgggg cacagcgggg
     3721 aggacctgcc gttggtttac cctgagaatg tgggggtgga actgctgaac agcagcaccg
     3781 tgcgcgtgag atggactttg gggggggggc ccaaagagct gcgggggcgt ctgaggggct
     3841 tccgggtgct gtattggcgt ttgggatggg tgggggagcg cagtcgtcga caagcccccc
     3901 ccgacccccc ccaaatcccc caaagcccgg ctgaagaccc ccccccattt ccccccgtgg
     3961 ctctgacagt ggggggggac gcgcgggggg cgctgctggg ggggctgcgg ccctggagcc
     4021 gttatcagct gcgggtgttg gtcttcaacg ggagggggga cggccccccc agcgaaccca
     4081 tcgccttcga gacccccgag ggagttcccg gcccccccga ggagctccgc gtggagcggt
     4141 tggacgacac cgccctctcc gtccattgga aacggccgcg gcatttaaac ggagtcctca
     4201 cgggatatgt actgagatac cagcaggtgg agccggcgct cggcctcccc caggaggctc
     4261 cgtacccccc cgggaccctc aatgcgaccc taagggggct gaatgcgcgc tcccgatacc
     4321 ggctggcgct gcgcgcgagc acgcgcgtcg ggagcggccc cgccctgcag acggtgggga
     4381 gcacgaaacc ggaaccggcc ctccccgctt tggagcgcgt tttggtgtcg gaggtcgggg
     4441 aggatttcac ggtgctgctg tggagtttgg tgcagcccca ggaggacgac gtggagttcg
     4501 aagtccaatt catgaataaa agcacggatg agccgtggcg cacttcgggc cgcgccaact
     4561 cctctttaag gcggtaccgt ctggaggggc tgcggcccgg caccgcctac cgagtccaat
     4621 tcgtgggccg gaaccgctct ggggaaaacg tggccttctg ggagagcgaa gtgcaaacca
     4681 acggcaccgt ggtgcgcagc ctggtggggg ggtttgccac caaggggtgg ttcatcggct
     4741 tcgtcagctc cgtggtgctc cttctcctca tcctcctcat cctctgcttc atcaaacgca
     4801 gcaagggggg caagtattcg gtgaaggaca aggaggacac gcaggtggac tctgaggcgc
     4861 ggcccatgaa ggatgagacc tttggggagt acaggtcgtt ggagagcgaa gcggaggaga
     4921 agggttcggc ttcgggttcc ggcgccggtt ccggtgtggg ttctccgggt cggggtccgt
     4981 gcgcggcggg cagcgaagac agcctggcgg ggtacggagg cagcggggat gtgcagttca
     5041 atgaggatgg atccttcatc gggcagtacc gcggacccgg agccggaccc ggcagctccg
     5101 gccctgccag cccctgtgct gggccccccc tggatttggt aagtaagctt cgaattctgc
     5161 agtcgacggt accgcgggcc cgggatccac cggtcgtgag caagggcgag gaggataaca
     5221 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     5281 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     5341 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     5401 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     5461 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     5521 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     5581 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     5641 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     5701 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     5761 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     5821 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     5881 gcatggacga gctgtacaag cggaccgtct aatctagata agtaatgatc ataatcagcc
     5941 atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     6001 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     6061 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6121 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcccc gggtaccgag
     6181 ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6241 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6301 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6361 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6421 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6481 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6541 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6601 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6661 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6721 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6781 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6841 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6901 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6961 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7021 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7081 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7141 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7201 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7261 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7321 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7381 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7441 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7501 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7561 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7621 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7681 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7741 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7801 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7861 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7921 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7981 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8041 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8101 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8161 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8221 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8281 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8341 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     8401 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     8461 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     8521 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     8581 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     8641 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     8701 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     8761 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
     8821 ttgggatctt tgcattggcc cacggctctc aggatgggga tgctcccctt cagcacccgg
     8881 ttccccttgg aaactgatgg tcctggctct gtggcatggc agtggcactg tgaggagccc
     8941 cctaccagca gcacacagtg gggttggcac tgccacgctc cggatgccgc gctctgatcc
     9001 aaccccataa tcaagggaac ccgaattgcc ccatcattgc ccccaccacc cccatcctgc
     9061 cgggccctca caccccacgc tgccttctgg tgacattccc cagcccaaac ccacggcttc
     9121 atggctaccg cggggcattt cccattgccg ccccattatc agctctgcac acctcccgct
     9181 gtacccatgc ctcgtggctg cccttctttg acgtataatc ttctaattaa tacccggcct
     9241 tgtcaaagtg gagcacaaac gttaattaat tccccagcag gcaggtaatt aacagtgtga
     9301 ctcccttttt gctgcgagtg gggctgatac agagagatgt ggcactatgg agcccacggg
     9361 gtcctggcac tgggtgccca cggaggtccc catgtgctgc agtgtcaccg cctccgagga
     9421 gacagtattg tccctgcggt gtccctgcag ctcagctctg tccacagggc cacctccagt
     9481 ttggagggga cacaatgcag ccccgatgca acccatcctc gcagcatccc agggacaaag
     9541 accccactgc aagaccgcac acagggctgg gtcccgctcc cctaatatct acagtgcttc
     9601 ttgcatggcc ccttaatcaa tgcagttaat cagcatgcgc tcatgcaccg ctctggagct
     9661 gcaaagcccc tcgcagcgct gctcaccaac accgcgcacc gccccggccc agcctgcagc
     9721 acgcgctgca aacaggaaag aaacaaaata ttgcccaaat gtaggcaaag gcattcggct
     9781 gccttgacct ccgccgggcc gggccctgcc tgactcagct ccttactcag cgctcgcttc
     9841 ctccctccgg ctgccaccgc cgcagcgcac accctgacaa agagtggccc ttaacgggct
     9901 ctgaggtgca cccagcagtg cactcagcag tccaagggcc ggcctggagg tttgcaccgc
     9961 tacgtgctga cattagcatt gaacttggcc ctgggtagtg ctgcaggccg ggcggggtgg
    10021 gtgtagagag tgcagcgcgc gttgcacccg gtgccccttc ccctcccttg catcccagca
    10081 ggctgcaccc cagcaccagg cccgtgcatg catgctcctg gtgttattgc agcctggtgc
    10141 atgcatgcgt cttgatggtg cagcgctgtg catgcatcct ccttggtgtg tagcagtcta
    10201 gtgcatgcat acccctcggt gttattgctg ctctgtgcac gcacgctcat tgtatcactt
    10261 catcccagtg catgcactca cactggagcg attgctgctc ggtgcacgca cactcattgt
    10321 atcactgcag ctcagtggct gcacgcacac cggtgttatt gctgctcggt gcgtgcatgc
    10381 acatcagtgt cgctgcagct cagtgcatgc acgctcattg cccatcgcta tccctgcctc
    10441 tcctgctggc gctccccggg aggtgacttc aaggggaccg caggaccacc tcgggggtgg
    10501 ggggagggct gcacacgcgg accccgctcc ccctccccaa caaagcactg tggaatcaaa
    10561 aaggggggag gggggatgga ggggcgcgtc acacccccgc cccacaccct cacc
//
