LOCUS       sequence-355813-       10807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of NgCAM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Nabb AT, Bentley M
  TITLE     NgCAM and VAMP2 Reveal that Direct Delivery and Dendritic 
            Degradation Maintain Axonal Polarity.
  JOURNAL   Mol Biol Cell. 2021 Nov 3:mbcE21080425. doi: 
            10.1091/mbc.E21-08-0425.
  PUBMED    34731031
REFERENCE   2  (bases 1 to 10807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2021 Nov 3:mbcE21080425. doi: 10.1091/mbc.E21-08-0425."
COMMENT     Sequence Label: pBa.SBP-NgCAM-GFP
FEATURES             Location/Qualifiers
     source          1..10807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1300..1309
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1453..1563
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             join(5377..5379,5380..5382,5383..6093)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5422..5443)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5683..5702)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6030..6051
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6226..6360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6263..6282)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6317..6336
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6391..6407)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6391..6407)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6404..6426)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6415..6431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6439..6445,6446..6463,6464..6469))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6484..6505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6622..6639)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6793..7381)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6873..6892)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7552..8343,8344..8412))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8175..8194
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8413..8517)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8585..8603
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8641..8663)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8763..8782
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8976..8998
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8990..9007
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8991..9007
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggn
      121 cgcgcgccag gcggggcggg gcggggcgrg gggsggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggrgg
      901 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagctt ggcgcgccag ccaccatggc tctgccccat
     1321 gggctcggcc tcctcctgct cctgttgctg ggggggcccg gagccgccat caccattccc
     1381 ccggagtatg gtgcacacga tttcctggcg tacggagatc taggaggatc tggaggcggc
     1441 tctggcggag gggacgagaa aaccaccggc tggagaggcg gccacgtggt ggagggcctg
     1501 gccggcgagc tggagcagct gagagccaga ctggagcacc accctcaggg ccagagagag
     1561 cctggaggag gttctggagg aggttctgga ggaggtgcta gcctgcagcc ccccgagctg
     1621 acggaggaac ccccggaaca actcgtggtc ttccccagtg atgacatcgt cctcaaatgc
     1681 gtggccaccg ggaacccccc cgtccagtac cgatggagcc gtgaggatca gcccttcgtc
     1741 cccgaggagc acgggggggt ctcggtggtc cccggatcgg ggactttggt catcaacgcc
     1801 acgttggccg cgcggctcca ggggcgcttc cgctgcttcg ccaccaacgc gttgggcacc
     1861 gctgtgtctc ccgaggccaa cgtcatcgcc gagaacactc cgcagtggcc gaaggagaag
     1921 gtgaccccgg tggaggtgga ggagggggac cccgtggtgc tgccctgtga cccccccgag
     1981 agcgctgtgc cccctaaaat ctattggctc aacagcgaca tcgttcacat cgctcaggac
     2041 gagagggtat cgatggggca ggatgggaac ctctacttct ccaacgccat ggtgggcgac
     2101 agccaccccg actacatctg ccacgctcac ttcctcggcc cccgcaccat catccagaag
     2161 gagcccctcg acctccgcgt ggcccccagt aatgcggttc ggtcccgccg cccccgcctg
     2221 ctgctgcccc gcgaccccca aacgaccacc atcgccctcc gggggggcag cgtcgtgttg
     2281 gagtgcatcg ctgaggggct ccccactcca tgggtccgat ggcggcggct gaatggcccc
     2341 ctccccaccc gggcggcgtt ggagaacttc aacaaaacgc tgcggctgtg gggggtgacg
     2401 gagagcgacg acggggagta cgaatgtgtg gctgagaacg ggagggggac ggccaggggc
     2461 acccacagcg tcaccgtgga ggccgcccca tattgggtgc ggcggccaca gagtggggtc
     2521 ttcgggccgg gggagacggc gaggctggac tgcgaggtgg gggggaaacc ccgaccccaa
     2581 atccaatgga gcatcaatgg ggtccccatc gacgcgctgc cgggcgcgga gcggcggtgg
     2641 ctgcggggcg gcgctttggt gcttccggag ctgcggccga acgacagcgc ggtgctgcag
     2701 tgcgaggcga ggaaccgcca cggccgccta ttggccaacg ccttcctgca cgtcgtggag
     2761 ctgccggtcc gaatgctgac ggcggatgag cagcgctacg aagtggtgga aaaccaaacg
     2821 gtgtttctgc actgcagaac cttcggggcc cccgcgccaa acgtcgagtg gctgaccccc
     2881 actttggagc cggctctgca ggacgatcga tccttcgtgt tcaccaatgg gagccttcgg
     2941 ctgagtgcgg tgcggggggg ggacgggggg gtctacacct gcatggccca aaacgcccac
     3001 agcaacggca gcctcacggc gctcctggag gtcagagccc ccacccgaat ttcggccccc
     3061 ccccgaagca ccaccgccaa aaaaggggag acggtgacct ttcactgcgg ggcgaccttt
     3121 gaccccgccg tgacccccgg ggagctgcga tggctgcggg gggggcagcc gctgcccgac
     3181 gacccccggt attcggtggc ggcggagacg ctgacggtgt ccaacgtgga ctatggggac
     3241 gaggggacca ttcagtgccg cgcctccacc cctctcgact ccgcggaggc cgaagcgcag
     3301 ctcagagtcg tgggccgccc cggccccatc cgggacctcc aagtgatgga ggtggacgaa
     3361 caccgcgtgc gcctcagctg gaccccgggg gacgaccata acagccccat agagaagttc
     3421 gtggtggagg aggaggaggg catcttcagc gcggggcgct tcgcggagcg gctgacggtt
     3481 ccgggggggc agccgtggac gccccccctc ccgctgtccc catacgggcg gttccgcttc
     3541 cgggtggtgg ccgttaacgc ctacgggagg ggggagcaca gcgcccccag cgcccccatc
     3601 gagacccccc ccgcggctcc ggagcgcaac ccgggggggg tccatgggga gggcaatgag
     3661 accggcaacc tcgtcatcac ctgggagccc ctcccccccc aggcctggaa cgccccctgg
     3721 gttcggtacc gcgtgcagtg gcggccattg gaggagcccg gcgggggggg cccttcgggg
     3781 gggttcccgt gggccgaaag caccgtggac gccccccccg tggtggtggg ggggctcccc
     3841 ccgttcagcc ccttccagat ccgcgtccag gccgtgaacg gagccgggaa gggaccggaa
     3901 gcgacccccg gcgtggggca cagcggggag gacctgccgt tggtttaccc tgagaatgtg
     3961 ggggtggaac tgctgaacag cagcaccgtg cgcgtgagat ggactttggg gggggggccc
     4021 aaagagctgc gggggcgtct gaggggcttc cgggtgctgt attggcgttt gggatgggtg
     4081 ggggagcgca gtcgtcgaca agcccccccc gacccccccc aaatccccca aagcccggct
     4141 gaagaccccc ccccatttcc ccccgtggct ctgacagtgg ggggggacgc gcggggggcg
     4201 ctgctggggg ggctgcggcc ctggagccgt tatcagctgc gggtgttggt cttcaacggg
     4261 aggggggacg gcccccccag cgaacccatc gccttcgaga cccccgaggg agttcccggc
     4321 ccccccgagg agctccgcgt ggagcggttg gacgacaccg ccctctccgt ccattggaaa
     4381 cggccgcggc atttaaacgg agtcctcacg ggatatgtac tgagatacca gcaggtggag
     4441 ccggcgctcg gcctccccca ggaggctccg tacccccccg ggaccctcaa tgcgacccta
     4501 agggggctga atgcgcgctc ccgataccgg ctggcgctgc gcgcgagcac gcgcgtcggg
     4561 agcggccccg ccctgcagac ggtggggagc acgaaaccgg aaccggccct ccccgctttg
     4621 gagcgcgttt tggtgtcgga ggtcggggag gatttcacgg tgctgctgtg gagtttggtg
     4681 cagccccagg aggacgacgt ggagttcgaa gtccaattca tgaataaaag cacggatgag
     4741 ccgtggcgca cttcgggccg cgccaactcc tctttaaggc ggtaccgtct ggaggggctg
     4801 cggcccggca ccgcctaccg agtccaattc gtgggccgga accgctctgg ggaaaacgtg
     4861 gccttctggg agagcgaagt gcaaaccaac ggcaccgtgg tgcgcagcct ggtggggggg
     4921 tttgccacca aggggtggtt catcggcttc gtcagctccg tggtgctcct tctcctcatc
     4981 ctcctcatcc tctgcttcat caaacgcagc aaggggggca agtattcggt gaaggacaag
     5041 gaggacacgc aggtggactc tgaggcgcgg cccatgaagg atgagacctt tggggagtac
     5101 aggtcgttgg agagcgaagc ggaggagaag ggttcggctt cgggttccgg cgccggttcc
     5161 ggtgtgggtt ctccgggtcg gggtccgtgc gcggcgggca gcgaagacag cctggcgggg
     5221 tacggaggca gcggggatgt gcagttcaat gaggatggat ccttcatcgg gcagtaccgc
     5281 ggacccggag ccggacccgg cagctccggc cctgccagcc cctgtgctgg gccccccctg
     5341 gatttggtaa gtaagcttgg cgcgcctagg cctaccatgg tgagcaaggg cggggagctg
     5401 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     5461 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     5521 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     5581 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5641 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     5701 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5761 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     5821 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     5881 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     5941 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     6001 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     6061 gggatcactc tcggcatgga cgagctgtac aagcggaccg tctaatctag ataagtaatg
     6121 atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac
     6181 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     6241 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6301 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     6361 cccgggtacc gagctcgaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     6421 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6481 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6541 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6601 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6661 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6721 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6781 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6841 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6901 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6961 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7021 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7081 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccattggc
     7141 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7201 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7261 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7321 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7381 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7441 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7501 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7561 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7621 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7681 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7741 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7801 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7861 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7921 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7981 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8041 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8101 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8161 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8221 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8281 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8341 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8401 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8461 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8521 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     8581 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     8641 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     8701 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     8761 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     8821 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     8881 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     8941 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     9001 ggccagtgcc aagttgggat ctttgcattg gcccacggct ctcaggatgg ggatgctccc
     9061 cttcagcacc cggttcccct tggaaactga tggtcctggc tctgtggcat ggcagtggca
     9121 ctgtgaggag ccccctacca gcagcacaca gtggggttgg cactgccacg ctccggatgc
     9181 cgcgctctga tccaacccca taatcaaggg aacccgaatt gccccatcat tgcccccacc
     9241 acccccatcc tgccgggccc tcacacccca cgctgccttc tggtgacatt ccccagccca
     9301 aacccacggc ttcatggcta ccgcggggca tttcccattg ccgccccatt atcagctctg
     9361 cacacctccc gctgtaccca tgcctcgtgg ctgcccttct ttgacgtata atcttctaat
     9421 taatacccgg ccttgtcaaa gtggagcaca aacgttaatt aattccccag caggcaggta
     9481 attaacagtg tgactccctt tttgctgcga gtggggctga tacagagaga tgtggcacta
     9541 tggagcccac ggggtcctgg cactgggtgc ccacggaggt ccccatgtgc tgcagtgtca
     9601 ccgcctccga ggagacagta ttgtccctgc ggtgtccctg cagctcagct ctgtccacag
     9661 ggccacctcc agtttggagg ggacacaatg cagccccgat gcaacccatc ctcgcagcat
     9721 cccagggaca aagaccccac tgcaagaccg cacacagggc tgggtcccgc tcccctaata
     9781 tctacagtgc ttcttgcatg gccccttaat caatgcagtt aatcagcatg cgctcatgca
     9841 ccgctctgga gctgcaaagc ccctcgcagc gctgctcacc aacaccgcgc accgccccgg
     9901 cccagcctgc agcacgcgct gcaaacagga aagaaacaaa atattgccca aatgtaggca
     9961 aaggcattcg gctgccttga cctccgccgg gccgggccct gcctgactca gctccttact
    10021 cagcgctcgc ttcctccctc cggctgccac cgccgcagcg cacaccctga caaagagtgg
    10081 cccttaacgg gctctgaggt gcacccagca gtgcactcag cagtccaagg gccggcctgg
    10141 aggtttgcac cgctacgtgc tgacattagc attgaacttg gccctgggta gtgctgcagg
    10201 ccgggcgggg tgggtgtaga gagtgcagcg cgcgttgcac ccggtgcccc ttcccctccc
    10261 ttgcatccca gcaggctgca ccccagcacc aggcccgtgc atgcatgctc ctggtgttat
    10321 tgcagcctgg tgcatgcatg cgtcttgatg gtgcagcgct gtgcatgcat cctccttggt
    10381 gtgtagcagt ctagtgcatg catacccctc ggtgttattg ctgctctgtg cacgcacgct
    10441 cattgtatca cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac
    10501 gcacactcat tgtatcactg cagctcagtg gctgcacgca caccggtgtt attgctgctc
    10561 ggtgcgtgca tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg
    10621 ctatccctgc ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc
    10681 acctcggggg tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca
    10741 ctgtggaatc aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac
    10801 cctcacc
//
