LOCUS       sequence-355858-       10187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmXRN1-P1339AP1342A-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, 
            Weichenrieder O, Coles M, Izaurralde E
  TITLE     A direct interaction between DCP1 and XRN1 couples mRNA decapping to
            5' exonucleolytic degradation.
  JOURNAL   Nat Struct Mol Biol. 2012 Dec;19(12):1324-31. doi: 
            10.1038/nsmb.2413. Epub 2012 Nov 11.
  PUBMED    23142987
REFERENCE   2  (bases 1 to 10187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2413";
            journalName: "Nat Struct Mol Biol"; date: "2012-12"; volume: "19"; 
            issue: "12"; pages: "1324-31"
COMMENT     Sequence Label: pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P
FEATURES             Location/Qualifiers
     source          1..10187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             7493..7534
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7544..7561
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7584..7601)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7634..7768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7658..7677)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7712..7731
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8369..8957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8449..8468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9128..9919,9920..9988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9751..9770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9989..10093)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10161..10179
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcgcat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 ctactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc cgcccaacat
     2641 gggtgtaccg aaatttttcc gatatatatc ggaacgctac ccctgtctgt cggagctggc
     2701 ccgtgaacat tgtattccgg aatttgataa cctgtatctg gatatgaatg gaatcgtgca
     2761 taattgtagc caccccgatg ataataatat tcatttccat cttgaagaag aacaaatctt
     2821 tcaagagata tttaattatg ttgataaact tttttatcta attaaacccc aacggctgtt
     2881 ttttctgagc gtagatggcg tcgccccgcg cgcgaaaatg aatcaacaac gatcgcgccg
     2941 ttttcgcacc gcccgggaag ccgaacaaca agaagcgaaa gccgcgcaac gcggggaact
     3001 gcgcgaacat gaacggtttg atagtaattg tatcaccccc gggacggagt ttatggtccg
     3061 cctccaagaa ggcctccgag cgttcctgaa gaccaaaatc agcaccgacc cactctggca
     3121 acgctgtacg gtcattctga gcggacaaga agccccggga gaaggagaac ataaaattat
     3181 ggattatatc cgctatatga aaacccaacc cgattacgac ccgaacacgc ggcattgtct
     3241 ctatggattg gatgcggact tgataatcct cggactgtgt acgcatgaac tccattttgt
     3301 cgtcctccga gaagaagtga aatttggccg aaatgtgaaa cggacgtcag tcgaagaaac
     3361 ccgatttttt ctcctccatc tcggcctcct gcgcgagtac ctcgagctgg agttcgacgc
     3421 cctgagaaca gacgagcaca aattggacat tgcgcagctt atcgacgact gggtgctgat
     3481 gggctttttg gtcggcaacg acttcattcc ccacctgccg tgcctgcaca tttcatcaaa
     3541 tgcactgccg ctgctctacc gcacctacat cggcatttac ccgaccctcg gcggcaacat
     3601 caacgagaac ggcaagctca acctgcgccg cttgcagatt ttcatatcgg ccctcaccga
     3661 ggtggagctg gatcacttca aggagcacgc cgacgacttg aagtacatga acaacaaatc
     3721 agaggcgttc gacatggacg tcggcgagat caccgagtct caaaacctcg actccgatct
     3781 cggagccctc atcaacaaga gcatgctgct ctacgatgac gacagcgagg aggactgctc
     3841 cgacgagaac gcggttcttc tgaaggagtt ccagaactac aagcgaaact tctaccgcaa
     3901 caagttcaag cgtgatccga atgatgaact gatcgaggaa ctgtgccacc actacgtcaa
     3961 tgcgctgcag tgggtgctcg actactacta ccgtggcgtg cagtcctggg actggtacta
     4021 tcccttccac tacactccat tcatcagcga cctcaagaac atcgagcagg tggagatcgc
     4081 cttccacatg ggcacgccct ttctgccatt ccagcagctt ctggcggtac tgccggcggc
     4141 cagtgcaaag cttctccctg tcgcctacca cgacctcatg ctgctgccga ccagcccgct
     4201 ggccgagttc tatccactgg agttcgagag cgacctcaat ggcaagaaac acgattggga
     4261 ggcggttgtg ctgataccct tcatcgatga gggtcggctg ctggccgcta tgctgccatg
     4321 cgaggctcag ctgtcgctgg aggagcgcga acgcaaccga cacggtccca tgtatgtgta
     4381 caaatacagt acggtggcgc agggtcccat gccagcgtat ccaccgctga gggctcttcc
     4441 cgtgctctac tgcacggagg tggccaagtg gtcgcacgaa atcgcagtca atctgccgta
     4501 cagcgtgtgc atcgagctac caaatgcggc gagaaccgtt ttcttcccgg gatttcccac
     4561 catgcagcat ctgcccttcg atttcgagct gcgcaacgac cgcgtcaagg tgttcgaaca
     4621 ggtcagtcgc aaccagaaca ttgtgctaaa accacgcaag cgccagctgg aggacacact
     4681 gacggcggtg gcctcccagt acctcgggaa ggtgatccac gtcggctggc cgcatctcgt
     4741 caaggcgata gtggtgcgcg tggccacgcg agatcagcgc gtcgacagcg agggaatcac
     4801 gctaaacgat tcgcggcgtt tcgactcgga atgcaaggca ctgcaggaac actttatcaa
     4861 tcgtatggga atacagtttg ccaactatga tgtgctcgtc tatgtgcgca cctttgccgg
     4921 caattcgacg gagttccgcg acaagggggc attgatggtg cgcgactcgt ggagcagctc
     4981 cgtcaccggc tatccagccc agggcgtggt ggccgatctg actgtgtggg aaagaatgcg
     5041 caagaatttc ctcaacgtgg agcactactt ccccgttggc agcacgatct tcttgatcac
     5101 ggatccgtat tacggcagcg agggaactgt gcaggaccca cggctggcct acaccaacgg
     5161 ccggattcaa gtcagcatca tggtgcgacc ggaaccgaaa gtaaatgcgg cacgtcagct
     5221 gcaggaagag cgggaccggg actatctgag caccttccag gtctgcaact tgctccgaat
     5281 cagtggccgc accctgggac gcctaagtgg caccgtgtgg gtggtgctcg gtccgcggcg
     5341 tcagaagatg gaaaacgtga ccaagcacaa cattggcctg cagctcaagt atccgcggca
     5401 gaacgaggag cgtgccggat actgcttccg cacgaacaac cagtggtact actccagcct
     5461 ggccgtggat ctgatgcgta actattgcca gcgttaccca gacgttatcg acttctttgg
     5521 cgactccaac gatcgcgccg agttcgtatt cgaacaggac gtgttcccca atgcggttgg
     5581 ccatcgccgc gtcgaggaac tggccaactg ggtgcgccag cagccgcaca tgaaagtgga
     5641 gcgcatctct tgcggctcca agacggtctg ccgcgagacc atcgaactgc tgatagccgc
     5701 cgtcgacgac ctgcgctcac tgcccgtcaa gcacgttaaa ctgcaggtga agccgcactt
     5761 gctcatcaag cccaatgtca cactgccaga tgtctaccga tcaaagcgcc cggtgcgtct
     5821 cttcgatcgc gtcgtaatcg tacgcaccat ttacatggtg cccgtgggca ctaagggcac
     5881 cgtgattggc attcatcccg ttacggaccc caacccggtc cgcctggagt gcgtccatgc
     5941 tgtcgacacg ttctgcaagg tgctgttcga cagccccgtt ccgaactgca acaacatcca
     6001 tggcatcgcc gaagaccgag tttacaaggt gcccgaaatt gccctggtga tcatcaagac
     6061 ggatgaggaa ggaaagaagc agaacgactg cgaactgccg gtgagggatc ctcagccgaa
     6121 ccaggcacag gacgagccag tccgcgcgac cagcagtcgc tatgtgaccg ccgcagggtc
     6181 cacctcggtc ccgatcacga tgaagaccca aattagcgac gaattcgtca agacgcgtag
     6241 cgatccgata gcccgtacgg attcgtacaa gccatcgtcg gagccaaaac cggtgccggt
     6301 gccggagcag attaccaact ggcgggaacg cgtcagtacg ccaacaaaca aaccgcagcc
     6361 tgcgccaaat aattggcgca taaatcggtc ttcatcacgt caacagggcg gtagtatttt
     6421 tgttgctccg cccacaaaaa ctcctgatgc agctgcaagc actgcttcca ctgcattcac
     6481 cgcagcctcg tccgcaacgc tgactcctct ggatcagacc ttggcgctca tgtcggtttt
     6541 gggcgtgggt gaggaccaga gctcgccgcc actccaggag gcggtgcagc agcaacgtcc
     6601 gccactactt caacagcaaa gagctccgtt tcccggacaa atgcccaatc tagccaagcc
     6661 agcgctcttc tggcagcaag aggcgcagaa gcaggaggcg ctgcagcagg aggcgcagca
     6721 gcaggaggcg cagaagaagc agcagcaggc gcatgcgcaa atggagcctg aacgtatcaa
     6781 tagccagcat ttctacagaa gcggccagac aggagcggcg ttgaatcagc cgccattggg
     6841 tgctccatca aaaaggcagt ggcatgagtg ggtgcacccg cgcatgcagc acgccaacgc
     6901 attccacgct ggcgtcaaca atggatatca gatgcgtccc aaaaagaata tagctgcgca
     6961 gagcaccttt aacaacaacg tgcacatgca cctccagcaa ccatattacc cgaatcagca
     7021 gcagcagcag cagcagcagc agccactcca gctgacggag atcaacaatg cgccaccgcg
     7081 atactcaacg atccaagatt ttgtgcccat acaggcgtac cgtcccaaga aattaaatcg
     7141 cgtccaacca gccggtcgtc aggacgtcga tgccaccaag aatcctagtc gttcgcctgt
     7201 gttgcagcaa ccgacgaacg agactataga cacaaaggcc agttctagtc ttccggtaca
     7261 atcagcaggc gaacaggtga ttggactgat gcaaacgctt gaaattaagc cggctgcgtc
     7321 gcagtccgaa tctgatgggg tctcaacagg cagtgcgaac gcgcccacag caacgacatc
     7381 atcccaggcg gtcaaccgaa ggaagcatcg agtgcctcgc atcggagcca agttcgactt
     7441 ggaatacata ctgcccgact ccccgcatcc aacttctaga gggcccttcg aaggtaagcc
     7501 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     7561 ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc
     7621 agcctcgatc gaggatccag acatgataag atacattgat gagtttggac aaaccacaac
     7681 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     7741 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     7801 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     7861 ggctgattat gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     7921 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     7981 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
     8041 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
     8101 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
     8161 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
     8221 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     8281 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggccagcaa aaggccagga
     8341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     8821 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9061 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9121 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9181 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9241 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9301 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9361 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9421 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9481 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9541 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9601 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9661 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9721 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     9781 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9841 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9901 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9961 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10021 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10081 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    10141 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgt
//
