LOCUS       sequence-355967-        9573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Zekri L, Huntzinger E, Heimstadt S, Izaurralde E
  TITLE     The silencing domain of GW182 interacts with PABPC1 to promote 
            translational repression and degradation of microRNA targets and is 
            required for target release.
  JOURNAL   Mol Cell Biol. 2009 Dec;29(23):6220-31. doi: 10.1128/MCB.01081-09. 
            Epub 2009 Sep 21.
  PUBMED    19797087
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01081-09"; journalName: "Mol Cell Biol"; date: 
            "2009-12"; volume: "29"; issue: "23"; pages: "6220-31"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-V5His6_H
FEATURES             Location/Qualifiers
     source          1..9573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2586..2651
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2679..2705
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6879..6920
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6930..6947
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6970..6987)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7020..7154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7044..7063)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7098..7117
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7755..8343)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7835..7854)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8514..9305,9306..9374))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9137..9156
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9375..9479)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9547..9565
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 ccaacatgga cgcacaaaca cgacgacgtg agcgtcgcgc tgagaaacaa gctcaatgga
     2641 aagctgcaaa cggtggatct cctccaagct tgatggctta cccatacgat gttccagatt
     2701 acgctatgcg tgaagccctt ttttcccaag atggctgggg ctgtcagcat gttaaccagg
     2761 atactaattg ggaagttccc agttcgccag aaccagccaa taaggatgca cccggtccac
     2821 caatgtggaa gccaagcatt aacaatggta ctgatctttg ggagtccaat ttgagaaacg
     2881 gaggtcagcc ggccgcacag caagttccaa agccgtcgtg gggtcataca ccatcctcta
     2941 acttaggtgg aacatggggt gaggacgacg atggcgccga tagtagtagt gtgtggactg
     3001 gaggagctgt tagcaacgcg ggatccggag ctgcagtggg agtaaaccaa gccggagtta
     3061 atgtcggtcc aggcggtgtt gtttcgtctg gcggacctca gtggggacaa ggtgtcgttg
     3121 gcgtcggact tggatcaact ggcggaaatg gttccagtaa cattacaggc tcttcgggcg
     3181 ttgccacggg aagcagtggc aattcaagta atgccggaaa tggatggggc gatccacgcg
     3241 agattcgtcc actgggcgtc ggaggatcga tggacatccg taacgtcgag caccgtggag
     3301 gaaatggatc aggcgccaca tccagtgacc cgcgtgatat ccgtatgatt gacccacgcg
     3361 atccaatccg gggcgaccca cgcggcattt caggacgcct caacggtacg tcagagatgt
     3421 ggggacacca cccgcagatg tcgcacaatc aactgcaggg aattaataag atggtcggac
     3481 agagcgttgc gacagctagt acgagcgttg gcacgtcagg atccggaatt ggaccaggcg
     3541 gaccgggacc aagcacggtt tcgggaaaca ttccgacgca atggggtcca gcccagcccg
     3601 ttagtgtcgg agttagcgga ccgaaggata tgtcgaagca aattagcggc tgggaagagc
     3661 cgtcgcctcc cccacaacgc cgtagcatcc caaattatga cgacggaacg tccctctggg
     3721 gacaacagac acgcgtcccg gccgctagtg gacattggaa ggatatgaca gactccattg
     3781 gacgcagcag ccacctgatg cgcggacaga gtcagaccgg cggaattggc attgctggag
     3841 ttggaaatag caatgttcca gtgggagcca atccaagtaa tcctataagc agtgtagttg
     3901 gacctcaagc ccggattcca tctgtgggcg gcgtacaaca caaaccagac ggcggcgcta
     3961 tgtgggtgca ttccggcaat gtaggtggca gaaataatgt tgctgctgtt actacttggg
     4021 gagatgacac tcatagcgtt aatgtcggcg ctcccagcag tggcagtgta tccagcaaca
     4081 attgggttga tgacaagtcc aactcaacct tggcacaaaa ctcttggagc gacccggccc
     4141 ctgttggagt tagttggggc aataagcaaa gcaaaccgcc aagcaatagt gcttcatcag
     4201 gttggagcac tgctgcgggc gtggtggatg gggttgatct aggatctgag tggaacacgc
     4261 acggggggat tattggaaaa tctcagcaac aacaaaaact agcgggactt aacgtgggaa
     4321 tggtgaacgt aattaacgcg gagatcatta agcaaagcaa gcaatacagg atccttgtcg
     4381 agaacggctt taaaaaggaa gatgtagagc gggcattagt gattgctaat atgaacatcg
     4441 aagaggcagc cgatatgctc cgtgccaact catccctatc aatggatggt tggcgtcgac
     4501 atgatgagtc ccttggatct tatgccgacc acaatagttc aacaagcagc ggtggatttg
     4561 ctggtcgtta cccggtcaac agtggacaac cttcaatgtc ctttcctcat aataacctta
     4621 tgaataacat gggaggtacc gctgttactg gaggtaacaa caatacaaac atgacagctt
     4681 tacaggtgca aaagtattta aatcaagggc aacatggtgt cgctgttgga ccgcaagccg
     4741 ttggtaattc ttcagcagta tctgtcggat ttggtcagaa cacgtctaac gcagcagtgg
     4801 caggagcagc ctctgcaaat atagcagcaa atacaaacaa ccaaccgtct ggtcagcaaa
     4861 ttcgcatgct aggccagcaa attcagttgg ccattcatag tggtttcata tctagtcaga
     4921 tattgactca accgctaact caaacaaccc ttaacctttt aaaccaactt cttagcaata
     4981 ttaagcatct ccaggctgca cagcaatccc ttacccgcgg gggaaatgtc aatccaatgg
     5041 cagtgaatgt ggctatatct aaatacaagc agcaaatcca gaatttacag aaccagataa
     5101 gtgcacaaca ggctgtgtat gtaaaacagc aaaatatgca accaacttca caacaacaac
     5161 agccccaaca acagcaactt ccttctgttc atctaagtaa ctcaggcaac gactatttaa
     5221 gaggtcacga tgcaataaat aatttgcaaa gcaacttttc tgagctcaat attaataagc
     5281 caagtggata tcaaggagcg tccaatcaac aatcccgatt aaatcagtgg aagcttccag
     5341 tattagataa ggagatcaac tctgacagta cggaattttc tcgtgcccca ggtgcaacga
     5401 aacaaaattt gacggccaac acaagcaaca taaactcttt gggtcttcaa aacgatagta
     5461 catggtcaac tggacgcagt attggtgacg gttggcctga tccctcatct gataacgaga
     5521 ataaagactg gtctgttgct cagccaactt cagcagcaac tgcttacact gatctggtcc
     5581 aagagtttga gccaggcaag ccatggaagg gttcacagat caaaagcata gaagatgatc
     5641 ccagcattac accaggaagc gttgctagat ctccattgtc tattaattcg acgccaaaag
     5701 atgctgacat atttgccaat accggtaaaa attcaccgac tgatttaccg ccactaagtt
     5761 tatcgtcgtc tacatggagt tttaatccaa accaaaatta tccgagtcac agttggtctg
     5821 acaatagtca acaatgtacc gccacttcgg agctttggac aagcccgcta aataaatcat
     5881 cgtctcgagg tcccccgcca ggattgactg ccaattcaaa taaatctgca aatagtaatg
     5941 cgtcaacgcc aacaactatt accggaggtg cgaatggatg gttacagcct cgaagtggcg
     6001 gtgttcaaac cacaaacact aattggacag gtggtaacac cacttggggc tccagttggt
     6061 tgcttttgaa aaatctaaca gcacagattg atggtcctac tttgcgtaca ctgtgtatgc
     6121 agcatgggcc ccttgtcagc tttcacccgt atttgaacca aggaattgcc ttatgtaaat
     6181 atactactcg tgaggaggcg aacaaggcgc aaatggcgtt aaacaactgt gtcctcgcca
     6241 acaccacaat atttgctgaa tctcccagcg agaacgaggt gcaaagcatt atgcagcact
     6301 taccacaaac tccttcctct acaagctcta gtggaactag tggtggcaac gtcggaggcg
     6361 tcggcacttc agccaataat gcaaacagtg gttctgcagc ttgtctgtcc ggaaacaata
     6421 gcggcaacgg aaacggcagc gcgagcggcg ccggcagcgg caacaatggc aacagtagct
     6481 gcaacaacag tgccgccggg gggggcagca gcagcaacaa cacgattacc actgtagcaa
     6541 attcgaatct tgttggttct agtggctctg tctcaaattc ctctggcgtt actgctaact
     6601 ctagtactgt ttctgtagtt agttgtacag cgagtgggaa ttccataaat ggggcaggta
     6661 ctgcaaacag ttctggttca aagagtagtg caaacaattt agctagcggc cagtctagcg
     6721 cttctaactt aactaatagc accaattcaa catggcgaca aactagccaa aaccaagctc
     6781 ttcaaagtca aagcaggcca tcaggcagag aagctgactt tgattatata tctctcgttt
     6841 attccattgt tgatgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     6901 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     6961 gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg
     7021 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     7081 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     7141 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     7201 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     7261 agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     7321 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     7381 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     7441 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     7501 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     7561 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     7621 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     7681 gatgacgacc atcagggaca gcttcaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7741 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7801 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7861 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7921 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7981 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8041 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8101 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8161 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     8221 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8281 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8341 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8401 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8461 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8521 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8581 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8641 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8701 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8761 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8821 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8881 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8941 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9001 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9061 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9121 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9181 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9241 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9301 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9361 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9421 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9481 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9541 tataaaaata ggcgtatcac gaggcccttt cgt
//
