LOCUS       sequence-355969-        9382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6Biso1_1-1256.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6Biso1_1-1256_H
FEATURES             Location/Qualifiers
     source          1..9382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    5086..5207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5097..5116)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5151..5170
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5393..5848
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5480..5499)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5690..5711
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5965..6322
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5965..6156
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5988..6008)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6173..6308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6373..7167
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6427..6446)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7037..7056
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7231..7279
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7320..7339)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7439..7461
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7499..7517)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7585..7689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7690..7758,7759..8550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7908..7927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8721..9309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9210..9229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc acgcgtggta
     1261 cctctagagt cgacatgaga gagaaggagc aagaaaggga agaacagtta atggaagaca
     1321 agaaaaggaa gaaagaggat aaaaagaaaa aagaagccac tcagaaggtc acggaacaaa
     1381 aaaccaaagt gcccgaagtg acgaaaccaa gtttaagcca accaacggcc gccagcccaa
     1441 ttggcagctc tccatcgcca ccagtcaatg gtggcaacaa tgccaaaagg gtggcagtgc
     1501 cgaacggaca accgccaagc gccgcccgct acatgcctcg ggaggtgccg ccgcgattcc
     1561 gttgccagca ggaccacaaa gtgttactaa aacgtgggca gccccctcca ccgtcctgca
     1621 tgctccttgg gggtggggca gggcctcctc cctgcacagc acctggagca aacccaaaca
     1681 acgcacaagt gacaggagcg ctgctgcaga gtgagagtgg gactgcgcca gactcaaccc
     1741 ttggaggtgc tgctgcttca aattatgcaa attccacttg gggctcggga gcctcctcca
     1801 acaacggcac ctcccccaac ccaattcaca tctgggacaa ggtgattgta gacgggtctg
     1861 acatggaaga gtggccttgt attgccagca aagacactga atcttcttcc gaaaacacca
     1921 ccgataacaa cagtgcctcg aaccctggct ctgagaagag cactctgcca ggaagcacca
     1981 ctagtaacaa aggaaaaggg agccagtgcc agtctgcaag ttctgggaac gaatgtaatc
     2041 ttggggtctg gaaatctgac cctaaggcta aatctgttca atcttccaac tctactacag
     2101 agaacaacaa tggactagga aattggagga atgtgagtgg tcaggataga attggacctg
     2161 gctctggctt cagcaacttt aacccaaata gcaacccatc tgcctggcca gcactggtcc
     2221 aagaaggaac ttctaggaaa ggggcattgg aaacagataa tagtaattcc agtgcacagg
     2281 ttagcacagt aggtcagaca tccagggaac agcagtcaaa gatggaaaat gcgggtgtta
     2341 attttgttgt ctctggcaga gaacaggctc aaattcataa cactgatgga ccaaaaaatg
     2401 gaaacactaa ctccttgaac ttaagttcac caaaccccat ggagaataag ggaatgccct
     2461 ttggaatggg cttggggaac acctccagga gcactgatgc cccttcacaa agcactggag
     2521 atcgaaagac tgggagtgtt ggatcttggg gtgcagctag ggggccttct ggaactgaca
     2581 cagtctctgg acaaagcaat tctggaaaca atgggaacaa tggaaaagag agagaggact
     2641 cctggaaagg agcttctgtt cagaaatcaa ctgggtcaaa aaatgactct tgggacaaca
     2701 ataacaggtc tacgggtggg tcctggaact ttggccccca ggactctaat gacaacaaat
     2761 ggggtgaagg gaacaaaatg acatctgggg tctctcaggg agaatggaaa cagccgactg
     2821 ggtctgatga gttgaaaatt ggagaatgga gtggtccaaa ccaaccaaat tctagcactg
     2881 gagcatggga caatcaaaag ggccaccccc tccctgaaaa ccaaggcaat gcccaggctc
     2941 cctgttgggg aagatcttcc agctccacag gaagtgaagt tggaggtcaa agcactggaa
     3001 gcaaccacaa agcaggaagt agtgacagtc ataactctgg ccgtcggtcg tacaggccca
     3061 cacatcctga ttgtcaggct gtcttgcaga ctcttttgag ccgaactgat ttggacccca
     3121 gggtgctctc aaacactggc tggggccaaa ctcaaattaa gcaggacaca gtgtgggaca
     3181 ttgaagaggt gccaaggcct gaggggaaat ctgacaaagg aactgagggg tgggagagcg
     3241 ctgccacaca gaccaagaac tcagggggct ggggagatgc acccagccaa agcaatcaaa
     3301 tgaagtctgg atggggggag ctctcagcct ctacagagtg gaaagacccc aagaacacag
     3361 gaggctggaa tgactacaag aacaacaact cttccaactg gggaggagga cgacctgatg
     3421 aaaagacacc ttcctcttgg aatgagaatc ccagcaagga tcaggggtgg ggaggtggac
     3481 gccagcccaa tcaaggatgg tcttctggaa agaatggttg gggggaggaa gtcgatcaga
     3541 caaaaaacag caattgggaa agttctgcaa gtaaacctgt gtctgggtgg ggtgaaggag
     3601 ggcagaatga aatcgggact tggggtaatg gtggcaatgc aagcctagct tcaaaaggtg
     3661 ggtgggagga ttgcaaaaga tccccagcat ggaatgagac gggccgacag cccaattcct
     3721 ggaataaaca acaccaacag cagcagcccc cacagcagcc gccgccacca caaccagagg
     3781 cttctggttc gtggggaggc ccacccccac cacctccagg caacgttcga ccttccaatt
     3841 ccagctggag cagcgggcca cagcctgcaa cacctaagga tgaggaaccc agtggttggg
     3901 aagagccatc cccacagtca attagtcgga aaatggacat tgatgatggc acttcagcat
     3961 ggggagaccc taacagttat aactacaaga atgtgaatct gtgggataag aattcccaag
     4021 ggggcccagc acctcgagaa ccaaacctgc ccaccccaat gaccagtaaa tcggcatcag
     4081 attccaaatc tatgcaagac ggctgggggg agagtgacgg gccagtcaca ggagctcgcc
     4141 accccagctg ggaagaggag gaggatggag gagtctggaa caccactggc tctcagggca
     4201 gtgcttcctc ccacaactca gcaagctggg gacaaggagg aaagaaacaa atgaagtgct
     4261 cactcaaagg aggaaacaat gattcatgga tgaatcctct tgccaaacag ttttcaaata
     4321 tgggattgct gagtcagact gaagataatc caagcagcaa aatggatttg tctgtaggaa
     4381 gcctttcaga taaaaaattt gatgtggaca agcgagcgat gaatctcggg gattttaatg
     4441 atatcatgag gaaggatcga tctgggttcc gtccacctaa ttccaaagac atgggaacca
     4501 cagatagtgg gccttatttt gagaagggcg gtagtcatgg tttgtttgga aacagcacag
     4561 cacaatcgag aggtctgcac acacccgtgc agccactaaa ttcttctccc agtctccggg
     4621 cgcaagtgcc tccccagttt atttcccccc aggtttctgc ctcaatgctc aagcagtttc
     4681 ccaacagtgg cctgagtcca ggtcttttca atgtggggcc ccagttatct cctcaacaaa
     4741 ttgccatgct gagccagctt ccacaaattc cccagtttca gttggcatgt cagcttctct
     4801 tgcagcagca gcaacagcag cagttgttac agaaccagag aaagatttct caagctgtac
     4861 gccaacagca agagcagcag ctggctcgaa tggtgagtgc actgcagcag cagcagcagc
     4921 agcagaggca gccaggcatg aagcactcgc cctctcatcc tgttgggccc aagccgcatc
     4981 tggacaacat ggtacccaac gcattgaatg tggggctccc agaccttcaa accaaagggg
     5041 cggccgcttc cctttagtga gggttaatgc ttcgagcaga catgataaga tacattgatg
     5101 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     5161 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     5221 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa
     5281 acctctacaa atgtggtaaa atccgataag gatcgatccg ggctggcgta atagcgaaga
     5341 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     5401 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5461 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5521 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5581 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5641 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5701 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5761 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5821 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     5881 gcggtatttc acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga
     5941 aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca
     6001 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6061 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6121 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6181 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6241 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6301 tggaggccta ggcttttgca aaaagcttga ttcttctgac acaacagtct cgaacttaag
     6361 gctagagcca ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     6421 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     6481 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     6541 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     6601 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     6661 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     6721 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     6781 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     6841 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     6901 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     6961 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     7021 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     7081 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     7141 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     7201 cgacgcccaa cctgccatca cgatggccgc aataaaatat ctttattttc attacatctg
     7261 tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc gcgtatggtg cactctcagt
     7321 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     7381 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7441 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     7501 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     7561 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7621 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7681 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7741 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7801 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7861 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7921 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7981 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8041 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8101 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8161 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8221 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8281 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8341 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8401 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8461 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8521 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8581 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8641 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8701 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8761 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8821 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8881 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8941 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9001 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9061 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9121 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9181 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9241 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9301 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9361 gctcacatgg ctcgacagat ct
//
